NaCl: for the safer in vivo use of antibacterial silver based nanoparticles

Mingzhuo Liu,1 Huiqing Zhang,1 Xiangwei Song,2 Chaochao Wei,2 Zhenfang Xiong,3 Fen Yu,2 Chen Li,4 Fanrong Ai,5 Guanghua Guo,1,* Xiaolei Wang2,* 1Department of Burns, The First Affiliated Hospital, Nanchang University, Nanchang, Jiangxi, China; 2Department of Translational Medicine, Nanchang Univers...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Liu MZ, Zhang HQ, Song XW, Wei CC, Xiong ZF, Yu F, Li C, Ai FR, Guo GH, Wang XL
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Ag nanoparticles NaCl Cytotoxcity Antibacterial Metabolism Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/6098b11cfbf8487f9bc94d6611ac9ba6
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6098b11cfbf8487f9bc94d6611ac9ba6
record_format	dspace
spelling	oai:doaj.org-article:6098b11cfbf8487f9bc94d6611ac9ba62021-12-02T00:31:38ZNaCl: for the safer in vivo use of antibacterial silver based nanoparticles1178-2013https://doaj.org/article/6098b11cfbf8487f9bc94d6611ac9ba62018-03-01T00:00:00Zhttps://www.dovepress.com/nacl-for-the-safer-in-vivo-use-of-antibacterial-silver-based-nanoparti-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Mingzhuo Liu,1 Huiqing Zhang,1 Xiangwei Song,2 Chaochao Wei,2 Zhenfang Xiong,3 Fen Yu,2 Chen Li,4 Fanrong Ai,5 Guanghua Guo,1,* Xiaolei Wang2,* 1Department of Burns, The First Affiliated Hospital, Nanchang University, Nanchang, Jiangxi, China; 2Department of Translational Medicine, Nanchang University, Nanchang, Jiangxi, China; 3Department of Pathology, The First Affiliated Hospital, Nanchang University, Nanchang, Jiangxi, China; 4Department of Orthopedic Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China; 5School of Mechanical & Electronic Engineering, Nanchang University, Nanchang, Jiangxi, China *These authors contributed equally to this work Background: As antibiotics progressively cease to be effective, silver based nanoparticles (SBNs), with broad antibacterial spectrum, might be the last line of defense against malicious bacteria. Unfortunately, there are still no proper SBNs-based strategies for in vivo antibacterial therapies. In this article, new carbon membrane packaged Ag nanoparticles (Ag-C) were synthesized. We assessed the effect of Ag-C with NaCl on size, cytotoxicity, antibacterial properties, metabolism and sepsis models.Methods: The size of Ag-C with NaCl was accessed with UV-vis, TEM and SEM. Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa were used to illustrate the antibacterial properties of SBNs affected by NaCl. L929 and 3T3 cell lines were cultured in vitro; CCK-8 assay was used to test cytotoxicity. Then, we explored the metabolism of Ag-C with NaCl in vivo. Finally, the effect of Ag-C with 4× NaCl on sepsis was observed.Results: NaCl could regulate the size of Ag-C. Ag-C exhibited superior antibacterial properties compared to similar sized pure Ag nanoparticles. Furthermore, the addition of NaCl could not only reduce the cytotoxicity of Ag-C, but could also continue to discharge Ag-C from major organs. Based on these factors, this method was used to treat a sepsis model (induced via cecal ligation and puncture), and it achieved satisfactory survival results.Conclusion: This discovery, though still in its infancy, could significantly improve the safety and feasibility of SBNs and could potentially play an important role in modern in vivo antibacterial applications. Thus, a new method to combating the growing threat from drug-resistant bacteria could be possible. NaCl is the key to excretion of SBNs after in vivo antibacterial use. Keywords: Ag nanoparticles, NaCl, cytotoxicity, antibacterial, metabolismLiu MZZhang HQSong XWWei CCXiong ZFYu FLi CAi FRGuo GHWang XLDove Medical PressarticleAg nanoparticlesNaClCytotoxcityAntibacterialMetabolismMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 1737-1748 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ag nanoparticles NaCl Cytotoxcity Antibacterial Metabolism Medicine (General) R5-920
spellingShingle	Ag nanoparticles NaCl Cytotoxcity Antibacterial Metabolism Medicine (General) R5-920 Liu MZ Zhang HQ Song XW Wei CC Xiong ZF Yu F Li C Ai FR Guo GH Wang XL NaCl: for the safer in vivo use of antibacterial silver based nanoparticles
description	Mingzhuo Liu,1 Huiqing Zhang,1 Xiangwei Song,2 Chaochao Wei,2 Zhenfang Xiong,3 Fen Yu,2 Chen Li,4 Fanrong Ai,5 Guanghua Guo,1,* Xiaolei Wang2,* 1Department of Burns, The First Affiliated Hospital, Nanchang University, Nanchang, Jiangxi, China; 2Department of Translational Medicine, Nanchang University, Nanchang, Jiangxi, China; 3Department of Pathology, The First Affiliated Hospital, Nanchang University, Nanchang, Jiangxi, China; 4Department of Orthopedic Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China; 5School of Mechanical & Electronic Engineering, Nanchang University, Nanchang, Jiangxi, China *These authors contributed equally to this work Background: As antibiotics progressively cease to be effective, silver based nanoparticles (SBNs), with broad antibacterial spectrum, might be the last line of defense against malicious bacteria. Unfortunately, there are still no proper SBNs-based strategies for in vivo antibacterial therapies. In this article, new carbon membrane packaged Ag nanoparticles (Ag-C) were synthesized. We assessed the effect of Ag-C with NaCl on size, cytotoxicity, antibacterial properties, metabolism and sepsis models.Methods: The size of Ag-C with NaCl was accessed with UV-vis, TEM and SEM. Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa were used to illustrate the antibacterial properties of SBNs affected by NaCl. L929 and 3T3 cell lines were cultured in vitro; CCK-8 assay was used to test cytotoxicity. Then, we explored the metabolism of Ag-C with NaCl in vivo. Finally, the effect of Ag-C with 4× NaCl on sepsis was observed.Results: NaCl could regulate the size of Ag-C. Ag-C exhibited superior antibacterial properties compared to similar sized pure Ag nanoparticles. Furthermore, the addition of NaCl could not only reduce the cytotoxicity of Ag-C, but could also continue to discharge Ag-C from major organs. Based on these factors, this method was used to treat a sepsis model (induced via cecal ligation and puncture), and it achieved satisfactory survival results.Conclusion: This discovery, though still in its infancy, could significantly improve the safety and feasibility of SBNs and could potentially play an important role in modern in vivo antibacterial applications. Thus, a new method to combating the growing threat from drug-resistant bacteria could be possible. NaCl is the key to excretion of SBNs after in vivo antibacterial use. Keywords: Ag nanoparticles, NaCl, cytotoxicity, antibacterial, metabolism
format	article
author	Liu MZ Zhang HQ Song XW Wei CC Xiong ZF Yu F Li C Ai FR Guo GH Wang XL
author_facet	Liu MZ Zhang HQ Song XW Wei CC Xiong ZF Yu F Li C Ai FR Guo GH Wang XL
author_sort	Liu MZ
title	NaCl: for the safer in vivo use of antibacterial silver based nanoparticles
title_short	NaCl: for the safer in vivo use of antibacterial silver based nanoparticles
title_full	NaCl: for the safer in vivo use of antibacterial silver based nanoparticles
title_fullStr	NaCl: for the safer in vivo use of antibacterial silver based nanoparticles
title_full_unstemmed	NaCl: for the safer in vivo use of antibacterial silver based nanoparticles
title_sort	nacl: for the safer in vivo use of antibacterial silver based nanoparticles
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/6098b11cfbf8487f9bc94d6611ac9ba6
work_keys_str_mv	AT liumz naclforthesaferinvivouseofantibacterialsilverbasednanoparticles AT zhanghq naclforthesaferinvivouseofantibacterialsilverbasednanoparticles AT songxw naclforthesaferinvivouseofantibacterialsilverbasednanoparticles AT weicc naclforthesaferinvivouseofantibacterialsilverbasednanoparticles AT xiongzf naclforthesaferinvivouseofantibacterialsilverbasednanoparticles AT yuf naclforthesaferinvivouseofantibacterialsilverbasednanoparticles AT lic naclforthesaferinvivouseofantibacterialsilverbasednanoparticles AT aifr naclforthesaferinvivouseofantibacterialsilverbasednanoparticles AT guogh naclforthesaferinvivouseofantibacterialsilverbasednanoparticles AT wangxl naclforthesaferinvivouseofantibacterialsilverbasednanoparticles
_version_	1718403678235262976

NaCl: for the safer in vivo use of antibacterial silver based nanoparticles

Ejemplares similares