Electrospun gelatin/PCL and collagen/PLCL scaffolds for vascular tissue engineering

Wei Fu,1,2,* Zhenling Liu,1,* Bei Feng,1,2 Renjie Hu,1 Xiaomin He,1 Hao Wang,1 Meng Yin,1 Huimin Huang,1 Haibo Zhang,1 Wei Wang11Department of Pediatric Cardiothoracic Surgery, 2Institute of Pediatric Translational Medicine, Shanghai Children's Medical Center, Shanghai Jiao Tong University...

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Autores principales:	Fu W, Liu Z, Feng B, Hu R, He X, Wang H, Yin M, Huang H, Zhang H, Wang W
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/609c48edf4144661bc6d49c97cac4549
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spelling	oai:doaj.org-article:609c48edf4144661bc6d49c97cac45492021-12-02T02:45:50ZElectrospun gelatin/PCL and collagen/PLCL scaffolds for vascular tissue engineering1178-2013https://doaj.org/article/609c48edf4144661bc6d49c97cac45492014-05-01T00:00:00Zhttp://www.dovepress.com/electrospun-gelatinpcl-and-collagenplcl-scaffolds-for-vascular-tissue--a16810https://doaj.org/toc/1178-2013 Wei Fu,1,2,* Zhenling Liu,1,* Bei Feng,1,2 Renjie Hu,1 Xiaomin He,1 Hao Wang,1 Meng Yin,1 Huimin Huang,1 Haibo Zhang,1 Wei Wang11Department of Pediatric Cardiothoracic Surgery, 2Institute of Pediatric Translational Medicine, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China*These authors contributed equally to this workAbstract: Electrospun hybrid nanofibers prepared using combinations of natural and synthetic polymers have been widely investigated in cardiovascular tissue engineering. In this study, electrospun gelatin/polycaprolactone (PCL) and collagen/poly(l-lactic acid-co-ε-caprolactone) (PLCL) scaffolds were successfully produced. Scanning electron micrographs showed that fibers of both membranes were smooth and homogeneous. Water contact angle measurements further demonstrated that both scaffolds were hydrophilic. To determine cell attachment and migration on the scaffolds, both hybrid scaffolds were seeded with human umbilical arterial smooth muscle cells. Scanning electron micrographs and MTT assays showed that the cells grew and proliferated well on both hybrid scaffolds. Gross observation of the transplanted scaffolds revealed that the engineered collagen/PLCL scaffolds were smoother and brighter than the gelatin/PCL scaffolds. Hematoxylin and eosin staining showed that the engineered blood vessels constructed by collagen/PLCL electrospun membranes formed relatively homogenous vessel-like tissues. Interestingly, Young's modulus for the engineered collagen/PLCL scaffolds was greater than for the gelatin/PCL scaffolds. Together, these results indicate that nanofibrous collagen/PLCL membranes with favorable mechanical and biological properties may be a desirable scaffold for vascular tissue engineering.Keywords: electrospinning, gelatin, collagen, polycaprolactone, poly(l-lactic acid-co-ε-caprolactone)Fu WLiu ZFeng BHu RHe XWang HYin MHuang HZhang HWang WDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 2335-2344 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Fu W Liu Z Feng B Hu R He X Wang H Yin M Huang H Zhang H Wang W Electrospun gelatin/PCL and collagen/PLCL scaffolds for vascular tissue engineering
description	Wei Fu,1,2,* Zhenling Liu,1,* Bei Feng,1,2 Renjie Hu,1 Xiaomin He,1 Hao Wang,1 Meng Yin,1 Huimin Huang,1 Haibo Zhang,1 Wei Wang11Department of Pediatric Cardiothoracic Surgery, 2Institute of Pediatric Translational Medicine, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China*These authors contributed equally to this workAbstract: Electrospun hybrid nanofibers prepared using combinations of natural and synthetic polymers have been widely investigated in cardiovascular tissue engineering. In this study, electrospun gelatin/polycaprolactone (PCL) and collagen/poly(l-lactic acid-co-ε-caprolactone) (PLCL) scaffolds were successfully produced. Scanning electron micrographs showed that fibers of both membranes were smooth and homogeneous. Water contact angle measurements further demonstrated that both scaffolds were hydrophilic. To determine cell attachment and migration on the scaffolds, both hybrid scaffolds were seeded with human umbilical arterial smooth muscle cells. Scanning electron micrographs and MTT assays showed that the cells grew and proliferated well on both hybrid scaffolds. Gross observation of the transplanted scaffolds revealed that the engineered collagen/PLCL scaffolds were smoother and brighter than the gelatin/PCL scaffolds. Hematoxylin and eosin staining showed that the engineered blood vessels constructed by collagen/PLCL electrospun membranes formed relatively homogenous vessel-like tissues. Interestingly, Young's modulus for the engineered collagen/PLCL scaffolds was greater than for the gelatin/PCL scaffolds. Together, these results indicate that nanofibrous collagen/PLCL membranes with favorable mechanical and biological properties may be a desirable scaffold for vascular tissue engineering.Keywords: electrospinning, gelatin, collagen, polycaprolactone, poly(l-lactic acid-co-ε-caprolactone)
format	article
author	Fu W Liu Z Feng B Hu R He X Wang H Yin M Huang H Zhang H Wang W
author_facet	Fu W Liu Z Feng B Hu R He X Wang H Yin M Huang H Zhang H Wang W
author_sort	Fu W
title	Electrospun gelatin/PCL and collagen/PLCL scaffolds for vascular tissue engineering
title_short	Electrospun gelatin/PCL and collagen/PLCL scaffolds for vascular tissue engineering
title_full	Electrospun gelatin/PCL and collagen/PLCL scaffolds for vascular tissue engineering
title_fullStr	Electrospun gelatin/PCL and collagen/PLCL scaffolds for vascular tissue engineering
title_full_unstemmed	Electrospun gelatin/PCL and collagen/PLCL scaffolds for vascular tissue engineering
title_sort	electrospun gelatin/pcl and collagen/plcl scaffolds for vascular tissue engineering
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/609c48edf4144661bc6d49c97cac4549
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Electrospun gelatin/PCL and collagen/PLCL scaffolds for vascular tissue engineering

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