Effect of chromosome tethering on nuclear organization in yeast.

Interphase chromosomes in Saccharomyces cerevisiae are tethered to the nuclear envelope at their telomeres and to the spindle pole body (SPB) at their centromeres. Using a polymer model of yeast chromosomes that includes these interactions, we show theoretically that telomere attachment to the nucle...

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Autores principales:	Barış Avşaroğlu, Gabriel Bronk, Susannah Gordon-Messer, Jungoh Ham, Debra A Bressan, James E Haber, Jane Kondev
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/609d73436daf462dadade1e315f509da
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spelling	oai:doaj.org-article:609d73436daf462dadade1e315f509da2021-11-25T06:08:34ZEffect of chromosome tethering on nuclear organization in yeast.1932-620310.1371/journal.pone.0102474https://doaj.org/article/609d73436daf462dadade1e315f509da2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25020108/?tool=EBIhttps://doaj.org/toc/1932-6203Interphase chromosomes in Saccharomyces cerevisiae are tethered to the nuclear envelope at their telomeres and to the spindle pole body (SPB) at their centromeres. Using a polymer model of yeast chromosomes that includes these interactions, we show theoretically that telomere attachment to the nuclear envelope is a major determinant of gene positioning within the nucleus only for genes within 10 kb of the telomeres. We test this prediction by measuring the distance between the SPB and the silent mating locus (HML) on chromosome III in wild-type and mutant yeast strains that contain altered chromosome-tethering interactions. In wild-type yeast cells we find that disruption of the telomere tether does not dramatically change the position of HML with respect to the SPB, in agreement with theoretical predictions. Alternatively, using a mutant strain with a synthetic tether that localizes an HML-proximal site to the nuclear envelope, we find a significant change in the SPB-HML distance, again as predicted by theory. Our study quantifies the importance of tethering at telomeres on the organization of interphase chromosomes in yeast, which has been shown to play a significant role in determining chromosome function such as gene expression and recombination.Barış AvşaroğluGabriel BronkSusannah Gordon-MesserJungoh HamDebra A BressanJames E HaberJane KondevPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 7, p e102474 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Barış Avşaroğlu Gabriel Bronk Susannah Gordon-Messer Jungoh Ham Debra A Bressan James E Haber Jane Kondev Effect of chromosome tethering on nuclear organization in yeast.
description	Interphase chromosomes in Saccharomyces cerevisiae are tethered to the nuclear envelope at their telomeres and to the spindle pole body (SPB) at their centromeres. Using a polymer model of yeast chromosomes that includes these interactions, we show theoretically that telomere attachment to the nuclear envelope is a major determinant of gene positioning within the nucleus only for genes within 10 kb of the telomeres. We test this prediction by measuring the distance between the SPB and the silent mating locus (HML) on chromosome III in wild-type and mutant yeast strains that contain altered chromosome-tethering interactions. In wild-type yeast cells we find that disruption of the telomere tether does not dramatically change the position of HML with respect to the SPB, in agreement with theoretical predictions. Alternatively, using a mutant strain with a synthetic tether that localizes an HML-proximal site to the nuclear envelope, we find a significant change in the SPB-HML distance, again as predicted by theory. Our study quantifies the importance of tethering at telomeres on the organization of interphase chromosomes in yeast, which has been shown to play a significant role in determining chromosome function such as gene expression and recombination.
format	article
author	Barış Avşaroğlu Gabriel Bronk Susannah Gordon-Messer Jungoh Ham Debra A Bressan James E Haber Jane Kondev
author_facet	Barış Avşaroğlu Gabriel Bronk Susannah Gordon-Messer Jungoh Ham Debra A Bressan James E Haber Jane Kondev
author_sort	Barış Avşaroğlu
title	Effect of chromosome tethering on nuclear organization in yeast.
title_short	Effect of chromosome tethering on nuclear organization in yeast.
title_full	Effect of chromosome tethering on nuclear organization in yeast.
title_fullStr	Effect of chromosome tethering on nuclear organization in yeast.
title_full_unstemmed	Effect of chromosome tethering on nuclear organization in yeast.
title_sort	effect of chromosome tethering on nuclear organization in yeast.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/609d73436daf462dadade1e315f509da
work_keys_str_mv	AT barısavsaroglu effectofchromosometetheringonnuclearorganizationinyeast AT gabrielbronk effectofchromosometetheringonnuclearorganizationinyeast AT susannahgordonmesser effectofchromosometetheringonnuclearorganizationinyeast AT jungohham effectofchromosometetheringonnuclearorganizationinyeast AT debraabressan effectofchromosometetheringonnuclearorganizationinyeast AT jamesehaber effectofchromosometetheringonnuclearorganizationinyeast AT janekondev effectofchromosometetheringonnuclearorganizationinyeast
_version_	1718414104190779392

Effect of chromosome tethering on nuclear organization in yeast.

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