A genetically encoded probe for imaging nascent and mature HA-tagged proteins in vivo

A genetically encoded probe for imaging nascent and mature HA-tagged proteins in vivo

Expression of genetically encoded antibodies for cell labelling is often limited by folding issues. Here, the authors engineer an anti-HA scFv antibody that works in the cellular environment and use it to track mRNA translation dynamics in living cells and to label proteins in live zebrafish embryos...

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Autores principales: Ning Zhao, Kouta Kamijo, Philip D. Fox, Haruka Oda, Tatsuya Morisaki, Yuko Sato, Hiroshi Kimura, Timothy J. Stasevich
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2019
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Acceso en línea:https://doaj.org/article/609e6cb7c33d4624985b65a876ea31c0
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spelling oai:doaj.org-article:609e6cb7c33d4624985b65a876ea31c02021-12-02T14:40:00ZA genetically encoded probe for imaging nascent and mature HA-tagged proteins in vivo10.1038/s41467-019-10846-12041-1723https://doaj.org/article/609e6cb7c33d4624985b65a876ea31c02019-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-10846-1https://doaj.org/toc/2041-1723Expression of genetically encoded antibodies for cell labelling is often limited by folding issues. Here, the authors engineer an anti-HA scFv antibody that works in the cellular environment and use it to track mRNA translation dynamics in living cells and to label proteins in live zebrafish embryos.Ning ZhaoKouta KamijoPhilip D. FoxHaruka OdaTatsuya MorisakiYuko SatoHiroshi KimuraTimothy J. StasevichNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-16 (2019)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Ning Zhao
Kouta Kamijo
Philip D. Fox
Haruka Oda
Tatsuya Morisaki
Yuko Sato
Hiroshi Kimura
Timothy J. Stasevich
A genetically encoded probe for imaging nascent and mature HA-tagged proteins in vivo
description Expression of genetically encoded antibodies for cell labelling is often limited by folding issues. Here, the authors engineer an anti-HA scFv antibody that works in the cellular environment and use it to track mRNA translation dynamics in living cells and to label proteins in live zebrafish embryos.
format article
author Ning Zhao
Kouta Kamijo
Philip D. Fox
Haruka Oda
Tatsuya Morisaki
Yuko Sato
Hiroshi Kimura
Timothy J. Stasevich
author_facet Ning Zhao
Kouta Kamijo
Philip D. Fox
Haruka Oda
Tatsuya Morisaki
Yuko Sato
Hiroshi Kimura
Timothy J. Stasevich
author_sort Ning Zhao
title A genetically encoded probe for imaging nascent and mature HA-tagged proteins in vivo
title_short A genetically encoded probe for imaging nascent and mature HA-tagged proteins in vivo
title_full A genetically encoded probe for imaging nascent and mature HA-tagged proteins in vivo
title_fullStr A genetically encoded probe for imaging nascent and mature HA-tagged proteins in vivo
title_full_unstemmed A genetically encoded probe for imaging nascent and mature HA-tagged proteins in vivo
title_sort genetically encoded probe for imaging nascent and mature ha-tagged proteins in vivo
publisher Nature Portfolio
publishDate 2019
url https://doaj.org/article/609e6cb7c33d4624985b65a876ea31c0
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