A genetically encoded probe for imaging nascent and mature HA-tagged proteins in vivo
Expression of genetically encoded antibodies for cell labelling is often limited by folding issues. Here, the authors engineer an anti-HA scFv antibody that works in the cellular environment and use it to track mRNA translation dynamics in living cells and to label proteins in live zebrafish embryos...
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Main Authors: | Ning Zhao, Kouta Kamijo, Philip D. Fox, Haruka Oda, Tatsuya Morisaki, Yuko Sato, Hiroshi Kimura, Timothy J. Stasevich |
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Format: | article |
Language: | EN |
Published: |
Nature Portfolio
2019
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Subjects: | |
Online Access: | https://doaj.org/article/609e6cb7c33d4624985b65a876ea31c0 |
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