Improvement of adenoviral vector-mediated gene transfer to airway epithelia by folate-modified anionic liposomes

Zhirong Zhong1,2, Yu Wan1, Jianfeng Han1, Sanjun Shi1, Zhirong Zhang1, Xun Sun11Key Laboratory of Drug Targeting and Drug Delivery Systems, Ministry of Education, West China School of Pharmacy, Sichuan University, Chengdu, Sichuan, People&#39;s Republic of China; 2Luzhou Medical College, Luz...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zhong Z, Wan Y, Han J, Shi S, Zhang Z, Sun X
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/60a37ea9bf8d4f4490cd7e78d623f598
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:60a37ea9bf8d4f4490cd7e78d623f598
record_format	dspace
spelling	oai:doaj.org-article:60a37ea9bf8d4f4490cd7e78d623f5982021-12-02T04:46:21ZImprovement of adenoviral vector-mediated gene transfer to airway epithelia by folate-modified anionic liposomes1176-91141178-2013https://doaj.org/article/60a37ea9bf8d4f4490cd7e78d623f5982011-05-01T00:00:00Zhttp://www.dovepress.com/improvement-of-adenoviral-vector-mediated-gene-transfer-to-airway-epit-a7529https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Zhirong Zhong1,2, Yu Wan1, Jianfeng Han1, Sanjun Shi1, Zhirong Zhang1, Xun Sun11Key Laboratory of Drug Targeting and Drug Delivery Systems, Ministry of Education, West China School of Pharmacy, Sichuan University, Chengdu, Sichuan, People&#39;s Republic of China; 2Luzhou Medical College, Luzhou, Sichuan, People&#39;s Republic of ChinaAbstract: Despite remarkable progress in the development of both viral and nonviral gene delivery vectors for airway disease treatment, poor gene transfer efficiency to the airway epithelium is a major obstacle in clinical application. To take advantage of the unique features of viral and nonviral vectors, we have developed complexes of adenovirus vector and anionic liposomes (AL-Ad5) by the calcium-induced phase change method. In the current study, based on the fact that there are overexpressed folate receptors on the surface of airway epithelia, we further modified the AL-Ad5 complexes with folate (F-AL-Ad5) to improve the transduction ability of Ad5 in airway epithelia. The transduction efficiencies of the obtained F-AL-Ad5 and AL-Ad5 complexes were assessed in primary-cultured airway epithelia in vitro. Our results indicated that compared with naked adenovirus vector, both AL-Ad5 and F-AL-Ad5 could significantly enhance the gene transduction efficiency of adenovirus vector in primary-cultured airway epithelial cells. Moreover, the enhancement mediated by F-AL-Ad5 was more dramatic than that by AL-Ad5. These results suggested that F-AL-Ad5 may be a useful strategy to deliver therapeutic genes to the airway epithelia and is promising in clinical application.Keywords: gene delivery, adenovirus vector, folate receptor, primary-cultured airway epitheliaZhong ZWan YHan JShi SZhang ZSun XDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 1083-1093 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Zhong Z Wan Y Han J Shi S Zhang Z Sun X Improvement of adenoviral vector-mediated gene transfer to airway epithelia by folate-modified anionic liposomes
description	Zhirong Zhong1,2, Yu Wan1, Jianfeng Han1, Sanjun Shi1, Zhirong Zhang1, Xun Sun11Key Laboratory of Drug Targeting and Drug Delivery Systems, Ministry of Education, West China School of Pharmacy, Sichuan University, Chengdu, Sichuan, People&#39;s Republic of China; 2Luzhou Medical College, Luzhou, Sichuan, People&#39;s Republic of ChinaAbstract: Despite remarkable progress in the development of both viral and nonviral gene delivery vectors for airway disease treatment, poor gene transfer efficiency to the airway epithelium is a major obstacle in clinical application. To take advantage of the unique features of viral and nonviral vectors, we have developed complexes of adenovirus vector and anionic liposomes (AL-Ad5) by the calcium-induced phase change method. In the current study, based on the fact that there are overexpressed folate receptors on the surface of airway epithelia, we further modified the AL-Ad5 complexes with folate (F-AL-Ad5) to improve the transduction ability of Ad5 in airway epithelia. The transduction efficiencies of the obtained F-AL-Ad5 and AL-Ad5 complexes were assessed in primary-cultured airway epithelia in vitro. Our results indicated that compared with naked adenovirus vector, both AL-Ad5 and F-AL-Ad5 could significantly enhance the gene transduction efficiency of adenovirus vector in primary-cultured airway epithelial cells. Moreover, the enhancement mediated by F-AL-Ad5 was more dramatic than that by AL-Ad5. These results suggested that F-AL-Ad5 may be a useful strategy to deliver therapeutic genes to the airway epithelia and is promising in clinical application.Keywords: gene delivery, adenovirus vector, folate receptor, primary-cultured airway epithelia
format	article
author	Zhong Z Wan Y Han J Shi S Zhang Z Sun X
author_facet	Zhong Z Wan Y Han J Shi S Zhang Z Sun X
author_sort	Zhong Z
title	Improvement of adenoviral vector-mediated gene transfer to airway epithelia by folate-modified anionic liposomes
title_short	Improvement of adenoviral vector-mediated gene transfer to airway epithelia by folate-modified anionic liposomes
title_full	Improvement of adenoviral vector-mediated gene transfer to airway epithelia by folate-modified anionic liposomes
title_fullStr	Improvement of adenoviral vector-mediated gene transfer to airway epithelia by folate-modified anionic liposomes
title_full_unstemmed	Improvement of adenoviral vector-mediated gene transfer to airway epithelia by folate-modified anionic liposomes
title_sort	improvement of adenoviral vector-mediated gene transfer to airway epithelia by folate-modified anionic liposomes
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/60a37ea9bf8d4f4490cd7e78d623f598
work_keys_str_mv	AT zhongz improvementofadenoviralvectormediatedgenetransfertoairwayepitheliabyfolatemodifiedanionicliposomes AT wany improvementofadenoviralvectormediatedgenetransfertoairwayepitheliabyfolatemodifiedanionicliposomes AT hanj improvementofadenoviralvectormediatedgenetransfertoairwayepitheliabyfolatemodifiedanionicliposomes AT shis improvementofadenoviralvectormediatedgenetransfertoairwayepitheliabyfolatemodifiedanionicliposomes AT zhangz improvementofadenoviralvectormediatedgenetransfertoairwayepitheliabyfolatemodifiedanionicliposomes AT sunx improvementofadenoviralvectormediatedgenetransfertoairwayepitheliabyfolatemodifiedanionicliposomes
_version_	1718401058014756864

Improvement of adenoviral vector-mediated gene transfer to airway epithelia by folate-modified anionic liposomes

Ejemplares similares