Electrospun tube reduces adhesion in rabbit Achilles tendon 12 weeks post-surgery without PAR-2 overexpression

Abstract One great challenge in surgical tendon repair is the minimization of peritendinous adhesions. An electrospun tube can serve as a physical barrier around a conventionally sutured tendon. Six New Zealand White rabbits had one Achilles tendon fully transsected and sutured by a 4-strand suture....

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Gabriella Meier Bürgisser, Olivera Evrova, Dorothea M. Heuberger, Petra Wolint, Julia Rieber, Iris Miescher, Reto A. Schüpbach, Pietro Giovanoli, Maurizio Calcagni, Johanna Buschmann
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/60a46f78b7f14be59a3484a156a97577
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:60a46f78b7f14be59a3484a156a97577
record_format	dspace
spelling	oai:doaj.org-article:60a46f78b7f14be59a3484a156a975772021-12-05T12:14:10ZElectrospun tube reduces adhesion in rabbit Achilles tendon 12 weeks post-surgery without PAR-2 overexpression10.1038/s41598-021-02780-42045-2322https://doaj.org/article/60a46f78b7f14be59a3484a156a975772021-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02780-4https://doaj.org/toc/2045-2322Abstract One great challenge in surgical tendon repair is the minimization of peritendinous adhesions. An electrospun tube can serve as a physical barrier around a conventionally sutured tendon. Six New Zealand White rabbits had one Achilles tendon fully transsected and sutured by a 4-strand suture. Another six rabbits had the same treatment, but with the additional electrospun DegraPol tube set around the sutured tendon. The adhesion formation to the surrounding tissue was investigated 12 weeks post-operation. Moreover, inflammation-related protease-activated receptor-2 (PAR-2) protein expression was assessed. Finally, rabbit Achilles tenocyte cultures were exposed to platelet-derived growth factor-BB (PDGF-BB), which mimicks the tendon healing environment, where PAR-2 gene expression was assessed as well as immunofluorescent staining intensity for F-actin and α-tubulin, respectively. At 12 weeks post-operation, the partially degraded DegraPol tube exhibited significantly lower adhesion formation (− 20%). PAR-2 protein expression was similar for time points 3 and 6 weeks, but increased at 12 weeks post-operation. In vitro cell culture experiments showed a significantly higher PAR-2 gene expression on day 3 after exposure to PDGF-BB, but not on day 7. The cytoskeleton of the tenocytes changed upon PDGF-BB stimulation, with signs of reorganization, and significantly decreased F-actin intensity. An electrospun DegraPol tube significantly reduces adhesion up to twelve weeks post-operation. At this time point, the tube is partially degraded, and a slight PAR-2 increase was detected in the DP treated tendons, which might however arise from particles of degrading DegraPol that were stained dark brown. PAR-2 gene expression in rabbit tenocytes reveals sensitivity at around day 10 after injury.Gabriella Meier BürgisserOlivera EvrovaDorothea M. HeubergerPetra WolintJulia RieberIris MiescherReto A. SchüpbachPietro GiovanoliMaurizio CalcagniJohanna BuschmannNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gabriella Meier Bürgisser Olivera Evrova Dorothea M. Heuberger Petra Wolint Julia Rieber Iris Miescher Reto A. Schüpbach Pietro Giovanoli Maurizio Calcagni Johanna Buschmann Electrospun tube reduces adhesion in rabbit Achilles tendon 12 weeks post-surgery without PAR-2 overexpression
description	Abstract One great challenge in surgical tendon repair is the minimization of peritendinous adhesions. An electrospun tube can serve as a physical barrier around a conventionally sutured tendon. Six New Zealand White rabbits had one Achilles tendon fully transsected and sutured by a 4-strand suture. Another six rabbits had the same treatment, but with the additional electrospun DegraPol tube set around the sutured tendon. The adhesion formation to the surrounding tissue was investigated 12 weeks post-operation. Moreover, inflammation-related protease-activated receptor-2 (PAR-2) protein expression was assessed. Finally, rabbit Achilles tenocyte cultures were exposed to platelet-derived growth factor-BB (PDGF-BB), which mimicks the tendon healing environment, where PAR-2 gene expression was assessed as well as immunofluorescent staining intensity for F-actin and α-tubulin, respectively. At 12 weeks post-operation, the partially degraded DegraPol tube exhibited significantly lower adhesion formation (− 20%). PAR-2 protein expression was similar for time points 3 and 6 weeks, but increased at 12 weeks post-operation. In vitro cell culture experiments showed a significantly higher PAR-2 gene expression on day 3 after exposure to PDGF-BB, but not on day 7. The cytoskeleton of the tenocytes changed upon PDGF-BB stimulation, with signs of reorganization, and significantly decreased F-actin intensity. An electrospun DegraPol tube significantly reduces adhesion up to twelve weeks post-operation. At this time point, the tube is partially degraded, and a slight PAR-2 increase was detected in the DP treated tendons, which might however arise from particles of degrading DegraPol that were stained dark brown. PAR-2 gene expression in rabbit tenocytes reveals sensitivity at around day 10 after injury.
format	article
author	Gabriella Meier Bürgisser Olivera Evrova Dorothea M. Heuberger Petra Wolint Julia Rieber Iris Miescher Reto A. Schüpbach Pietro Giovanoli Maurizio Calcagni Johanna Buschmann
author_facet	Gabriella Meier Bürgisser Olivera Evrova Dorothea M. Heuberger Petra Wolint Julia Rieber Iris Miescher Reto A. Schüpbach Pietro Giovanoli Maurizio Calcagni Johanna Buschmann
author_sort	Gabriella Meier Bürgisser
title	Electrospun tube reduces adhesion in rabbit Achilles tendon 12 weeks post-surgery without PAR-2 overexpression
title_short	Electrospun tube reduces adhesion in rabbit Achilles tendon 12 weeks post-surgery without PAR-2 overexpression
title_full	Electrospun tube reduces adhesion in rabbit Achilles tendon 12 weeks post-surgery without PAR-2 overexpression
title_fullStr	Electrospun tube reduces adhesion in rabbit Achilles tendon 12 weeks post-surgery without PAR-2 overexpression
title_full_unstemmed	Electrospun tube reduces adhesion in rabbit Achilles tendon 12 weeks post-surgery without PAR-2 overexpression
title_sort	electrospun tube reduces adhesion in rabbit achilles tendon 12 weeks post-surgery without par-2 overexpression
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/60a46f78b7f14be59a3484a156a97577
work_keys_str_mv	AT gabriellameierburgisser electrospuntubereducesadhesioninrabbitachillestendon12weekspostsurgerywithoutpar2overexpression AT oliveraevrova electrospuntubereducesadhesioninrabbitachillestendon12weekspostsurgerywithoutpar2overexpression AT dorotheamheuberger electrospuntubereducesadhesioninrabbitachillestendon12weekspostsurgerywithoutpar2overexpression AT petrawolint electrospuntubereducesadhesioninrabbitachillestendon12weekspostsurgerywithoutpar2overexpression AT juliarieber electrospuntubereducesadhesioninrabbitachillestendon12weekspostsurgerywithoutpar2overexpression AT irismiescher electrospuntubereducesadhesioninrabbitachillestendon12weekspostsurgerywithoutpar2overexpression AT retoaschupbach electrospuntubereducesadhesioninrabbitachillestendon12weekspostsurgerywithoutpar2overexpression AT pietrogiovanoli electrospuntubereducesadhesioninrabbitachillestendon12weekspostsurgerywithoutpar2overexpression AT mauriziocalcagni electrospuntubereducesadhesioninrabbitachillestendon12weekspostsurgerywithoutpar2overexpression AT johannabuschmann electrospuntubereducesadhesioninrabbitachillestendon12weekspostsurgerywithoutpar2overexpression
_version_	1718372135720714240

Electrospun tube reduces adhesion in rabbit Achilles tendon 12 weeks post-surgery without PAR-2 overexpression

Ejemplares similares