Optimizing and evaluating PCR-based pooled screening during COVID-19 pandemics

Abstract Population screening played a substantial role in safely reopening the economy and avoiding new outbreaks of COVID-19. PCR-based pooled screening makes it possible to test the population with limited resources by pooling multiple individual samples. Our study compared different population-w...

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Autores principales:	Jiali Yu, Yiduo Huang, Zuo-Jun Shen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/60a8813644c74fe1bf851061521bbe84
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spelling	oai:doaj.org-article:60a8813644c74fe1bf851061521bbe842021-11-08T10:55:50ZOptimizing and evaluating PCR-based pooled screening during COVID-19 pandemics10.1038/s41598-021-01065-02045-2322https://doaj.org/article/60a8813644c74fe1bf851061521bbe842021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01065-0https://doaj.org/toc/2045-2322Abstract Population screening played a substantial role in safely reopening the economy and avoiding new outbreaks of COVID-19. PCR-based pooled screening makes it possible to test the population with limited resources by pooling multiple individual samples. Our study compared different population-wide screening methods as transmission-mitigating interventions, including pooled PCR, individual PCR, and antigen screening. Incorporating testing-isolation process and individual-level viral load trajectories into an epidemic model, we further studied the impacts of testing-isolation on test sensitivities. Results show that the testing-isolation process could maintain a stable test sensitivity during the outbreak by removing most infected individuals, especially during the epidemic decline. Moreover, we compared the efficiency, accuracy, and cost of different screening methods during the pandemic. Our results show that PCR-based pooled screening is cost-effective in reversing the pandemic at low prevalence. When the prevalence is high, PCR-based pooled screening may not stop the outbreak. In contrast, antigen screening with sufficient frequency could reverse the epidemic, despite the high cost and the large numbers of false positives in the screening process.Jiali YuYiduo HuangZuo-Jun ShenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jiali Yu Yiduo Huang Zuo-Jun Shen Optimizing and evaluating PCR-based pooled screening during COVID-19 pandemics
description	Abstract Population screening played a substantial role in safely reopening the economy and avoiding new outbreaks of COVID-19. PCR-based pooled screening makes it possible to test the population with limited resources by pooling multiple individual samples. Our study compared different population-wide screening methods as transmission-mitigating interventions, including pooled PCR, individual PCR, and antigen screening. Incorporating testing-isolation process and individual-level viral load trajectories into an epidemic model, we further studied the impacts of testing-isolation on test sensitivities. Results show that the testing-isolation process could maintain a stable test sensitivity during the outbreak by removing most infected individuals, especially during the epidemic decline. Moreover, we compared the efficiency, accuracy, and cost of different screening methods during the pandemic. Our results show that PCR-based pooled screening is cost-effective in reversing the pandemic at low prevalence. When the prevalence is high, PCR-based pooled screening may not stop the outbreak. In contrast, antigen screening with sufficient frequency could reverse the epidemic, despite the high cost and the large numbers of false positives in the screening process.
format	article
author	Jiali Yu Yiduo Huang Zuo-Jun Shen
author_facet	Jiali Yu Yiduo Huang Zuo-Jun Shen
author_sort	Jiali Yu
title	Optimizing and evaluating PCR-based pooled screening during COVID-19 pandemics
title_short	Optimizing and evaluating PCR-based pooled screening during COVID-19 pandemics
title_full	Optimizing and evaluating PCR-based pooled screening during COVID-19 pandemics
title_fullStr	Optimizing and evaluating PCR-based pooled screening during COVID-19 pandemics
title_full_unstemmed	Optimizing and evaluating PCR-based pooled screening during COVID-19 pandemics
title_sort	optimizing and evaluating pcr-based pooled screening during covid-19 pandemics
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/60a8813644c74fe1bf851061521bbe84
work_keys_str_mv	AT jialiyu optimizingandevaluatingpcrbasedpooledscreeningduringcovid19pandemics AT yiduohuang optimizingandevaluatingpcrbasedpooledscreeningduringcovid19pandemics AT zuojunshen optimizingandevaluatingpcrbasedpooledscreeningduringcovid19pandemics
_version_	1718442527057510400

Optimizing and evaluating PCR-based pooled screening during COVID-19 pandemics

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