Development of two enzyme-linked immunosorbent assay formats for thifluzamide residues’ analysis based on distinct polyclonal antibodies

Haptens 2-Methyl-4-(trifluoromethyl)thiazole-5-carboxylic acid and 2,6-Dibromo-4-(trifluoromethoxy)aniline, the two moieties of thifluzamide, were conjugated with carrier proteins for the synthesis of artificial antigens. Two distinct anti-thifluzamide polyclonal antibodies (PAb-1 and PAb-2) were pr...

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Autores principales:	Xiaoxiao Li, Yun Zhao, Shumao Ding, Huihui You, Weiyong Feng, Xu Yang, Junlin Yuan
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2018
Materias:	thifluzamide polyclonal antibody elisa two formats analysis Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/60a96213f992405f9b0be9c5f2ea2910
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spelling	oai:doaj.org-article:60a96213f992405f9b0be9c5f2ea29102021-11-26T11:19:46ZDevelopment of two enzyme-linked immunosorbent assay formats for thifluzamide residues’ analysis based on distinct polyclonal antibodies0954-01051465-344310.1080/09540105.2017.1371115https://doaj.org/article/60a96213f992405f9b0be9c5f2ea29102018-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2017.1371115https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443Haptens 2-Methyl-4-(trifluoromethyl)thiazole-5-carboxylic acid and 2,6-Dibromo-4-(trifluoromethoxy)aniline, the two moieties of thifluzamide, were conjugated with carrier proteins for the synthesis of artificial antigens. Two distinct anti-thifluzamide polyclonal antibodies (PAb-1 and PAb-2) were produced from the immunized female Balb/c mice. The indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) in two formats based on the PAbs was developed for thifluzamide analysis. The concentration of 50% inhibition (IC50) of ELISA-1 was 1.39 mg L−1 and its limit of detection (LOD) was 0.082 mg L−1. Meanwhile, ELISA-2 had a similar IC50 of 1.96 mg L−1 and a LOD of 0.074 mg L−1 as ELISA-1. Both the raised PAbs exhibited high specificity to thifluzamide. The recoveries for spiked samples including water and wheat ranged from 72.0% to 128.4%, and the accuracy of ELISA was confirmed by high-performance liquid chromatography. In summary, the ic-ELISA might be a promising tool for simple, sensitive and rapid detection of thifluzamide residues in real samples.Xiaoxiao LiYun ZhaoShumao DingHuihui YouWeiyong FengXu YangJunlin YuanTaylor & Francis Grouparticlethifluzamidepolyclonal antibodyelisatwo formatsanalysisAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 29, Iss 1, Pp 267-280 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	thifluzamide polyclonal antibody elisa two formats analysis Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
spellingShingle	thifluzamide polyclonal antibody elisa two formats analysis Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 Xiaoxiao Li Yun Zhao Shumao Ding Huihui You Weiyong Feng Xu Yang Junlin Yuan Development of two enzyme-linked immunosorbent assay formats for thifluzamide residues’ analysis based on distinct polyclonal antibodies
description	Haptens 2-Methyl-4-(trifluoromethyl)thiazole-5-carboxylic acid and 2,6-Dibromo-4-(trifluoromethoxy)aniline, the two moieties of thifluzamide, were conjugated with carrier proteins for the synthesis of artificial antigens. Two distinct anti-thifluzamide polyclonal antibodies (PAb-1 and PAb-2) were produced from the immunized female Balb/c mice. The indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) in two formats based on the PAbs was developed for thifluzamide analysis. The concentration of 50% inhibition (IC50) of ELISA-1 was 1.39 mg L−1 and its limit of detection (LOD) was 0.082 mg L−1. Meanwhile, ELISA-2 had a similar IC50 of 1.96 mg L−1 and a LOD of 0.074 mg L−1 as ELISA-1. Both the raised PAbs exhibited high specificity to thifluzamide. The recoveries for spiked samples including water and wheat ranged from 72.0% to 128.4%, and the accuracy of ELISA was confirmed by high-performance liquid chromatography. In summary, the ic-ELISA might be a promising tool for simple, sensitive and rapid detection of thifluzamide residues in real samples.
format	article
author	Xiaoxiao Li Yun Zhao Shumao Ding Huihui You Weiyong Feng Xu Yang Junlin Yuan
author_facet	Xiaoxiao Li Yun Zhao Shumao Ding Huihui You Weiyong Feng Xu Yang Junlin Yuan
author_sort	Xiaoxiao Li
title	Development of two enzyme-linked immunosorbent assay formats for thifluzamide residues’ analysis based on distinct polyclonal antibodies
title_short	Development of two enzyme-linked immunosorbent assay formats for thifluzamide residues’ analysis based on distinct polyclonal antibodies
title_full	Development of two enzyme-linked immunosorbent assay formats for thifluzamide residues’ analysis based on distinct polyclonal antibodies
title_fullStr	Development of two enzyme-linked immunosorbent assay formats for thifluzamide residues’ analysis based on distinct polyclonal antibodies
title_full_unstemmed	Development of two enzyme-linked immunosorbent assay formats for thifluzamide residues’ analysis based on distinct polyclonal antibodies
title_sort	development of two enzyme-linked immunosorbent assay formats for thifluzamide residues’ analysis based on distinct polyclonal antibodies
publisher	Taylor & Francis Group
publishDate	2018
url	https://doaj.org/article/60a96213f992405f9b0be9c5f2ea2910
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_version_	1718409536834895872

Development of two enzyme-linked immunosorbent assay formats for thifluzamide residues’ analysis based on distinct polyclonal antibodies

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