EVALUATION OF MICROVESICLES FORMED BY NATURAL KILLER (NK) CELLS USING FLOW CYTOMETRY

As a result of activation and/or apoptosis, the cells can form microvesicles (MV) from 100 nm up to 1000 nm in size. Nowadays, the attention is being increasingly focused on dynamic detection and evaluation of leukocyte-derived microvesicles by their contents. In this regard, determination of microv...

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Autores principales:	V. A. Mikhailova, K. L. Belyakova, L. P. Vyazmina, A. R. Sheveleva, S. A. Selkov, D. I. Sokolov
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2018
Materias:	nk cells flow cytometry microvesicles microparticles cell culture nk-92 cell surface receptors Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/60a9edc57c0642f099f7cd2875c27de7
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spelling	oai:doaj.org-article:60a9edc57c0642f099f7cd2875c27de72021-11-18T08:03:47ZEVALUATION OF MICROVESICLES FORMED BY NATURAL KILLER (NK) CELLS USING FLOW CYTOMETRY1563-06252313-741X10.15789/1563-0625-2018-2-251-254https://doaj.org/article/60a9edc57c0642f099f7cd2875c27de72018-03-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1482https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAs a result of activation and/or apoptosis, the cells can form microvesicles (MV) from 100 nm up to 1000 nm in size. Nowadays, the attention is being increasingly focused on dynamic detection and evaluation of leukocyte-derived microvesicles by their contents. In this regard, determination of microvesicles formed by NK cells is of utmost interest. The main function of these population is to induce apoptosis of virus-infected and tumor cells. At the present time, there is no direct evidence of the NK cells ability to produce microvesicles. This investigation was performed in order to estimate contents of NK cell-derived microvesicles using highprecision flow cytometric approach. It has been shown that the high-precision flow cytometry allows to detect microvesicles formed by NK cells, ranging from 200 to 1000 nm in size. It was demonstrated that incubation of NK cells in the presence of TNFα did not affect the relative value of microvesicles, however, being associated with increased intensity of CD95 expression on microvesicles. Hence, the high-precision flow cytometry can be used to detect microvesicles and to determine their phenotype.V. A. MikhailovaK. L. BelyakovaL. P. VyazminaA. R. ShevelevaS. A. SelkovD. I. SokolovSPb RAACIarticlenk cellsflow cytometrymicrovesiclesmicroparticlescell culturenk-92cell surface receptorsImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 20, Iss 2, Pp 251-254 (2018)
institution	DOAJ
collection	DOAJ
language	RU
topic	nk cells flow cytometry microvesicles microparticles cell culture nk-92 cell surface receptors Immunologic diseases. Allergy RC581-607
spellingShingle	nk cells flow cytometry microvesicles microparticles cell culture nk-92 cell surface receptors Immunologic diseases. Allergy RC581-607 V. A. Mikhailova K. L. Belyakova L. P. Vyazmina A. R. Sheveleva S. A. Selkov D. I. Sokolov EVALUATION OF MICROVESICLES FORMED BY NATURAL KILLER (NK) CELLS USING FLOW CYTOMETRY
description	As a result of activation and/or apoptosis, the cells can form microvesicles (MV) from 100 nm up to 1000 nm in size. Nowadays, the attention is being increasingly focused on dynamic detection and evaluation of leukocyte-derived microvesicles by their contents. In this regard, determination of microvesicles formed by NK cells is of utmost interest. The main function of these population is to induce apoptosis of virus-infected and tumor cells. At the present time, there is no direct evidence of the NK cells ability to produce microvesicles. This investigation was performed in order to estimate contents of NK cell-derived microvesicles using highprecision flow cytometric approach. It has been shown that the high-precision flow cytometry allows to detect microvesicles formed by NK cells, ranging from 200 to 1000 nm in size. It was demonstrated that incubation of NK cells in the presence of TNFα did not affect the relative value of microvesicles, however, being associated with increased intensity of CD95 expression on microvesicles. Hence, the high-precision flow cytometry can be used to detect microvesicles and to determine their phenotype.
format	article
author	V. A. Mikhailova K. L. Belyakova L. P. Vyazmina A. R. Sheveleva S. A. Selkov D. I. Sokolov
author_facet	V. A. Mikhailova K. L. Belyakova L. P. Vyazmina A. R. Sheveleva S. A. Selkov D. I. Sokolov
author_sort	V. A. Mikhailova
title	EVALUATION OF MICROVESICLES FORMED BY NATURAL KILLER (NK) CELLS USING FLOW CYTOMETRY
title_short	EVALUATION OF MICROVESICLES FORMED BY NATURAL KILLER (NK) CELLS USING FLOW CYTOMETRY
title_full	EVALUATION OF MICROVESICLES FORMED BY NATURAL KILLER (NK) CELLS USING FLOW CYTOMETRY
title_fullStr	EVALUATION OF MICROVESICLES FORMED BY NATURAL KILLER (NK) CELLS USING FLOW CYTOMETRY
title_full_unstemmed	EVALUATION OF MICROVESICLES FORMED BY NATURAL KILLER (NK) CELLS USING FLOW CYTOMETRY
title_sort	evaluation of microvesicles formed by natural killer (nk) cells using flow cytometry
publisher	SPb RAACI
publishDate	2018
url	https://doaj.org/article/60a9edc57c0642f099f7cd2875c27de7
work_keys_str_mv	AT vamikhailova evaluationofmicrovesiclesformedbynaturalkillernkcellsusingflowcytometry AT klbelyakova evaluationofmicrovesiclesformedbynaturalkillernkcellsusingflowcytometry AT lpvyazmina evaluationofmicrovesiclesformedbynaturalkillernkcellsusingflowcytometry AT arsheveleva evaluationofmicrovesiclesformedbynaturalkillernkcellsusingflowcytometry AT saselkov evaluationofmicrovesiclesformedbynaturalkillernkcellsusingflowcytometry AT disokolov evaluationofmicrovesiclesformedbynaturalkillernkcellsusingflowcytometry
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EVALUATION OF MICROVESICLES FORMED BY NATURAL KILLER (NK) CELLS USING FLOW CYTOMETRY

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