EFFICIENCY OF THE FORMATION OF ANTITUMOR IMMUNE RESPONSES IN THE SYSTEM OF PREVENTIVE VACCINATION OF MICE WITH TESTICULAR ANTIGENS

Аppearance of a malignant tumor is associated with impaired mechanisms of proliferation, differentiation, apoptosis ability. However, these changes are not enough for immune system to recognize and destroy mutated cells. Weak immunogenicity of tumor-associated antigens (TAA) and the insufficiency of...

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Autores principales:	A. B. Dorzhieva, T. S. Khabalova, Yu. E. Androsova, E. A. Kaschenko, I. P. Ivanova, G. V. Seledtsova
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2021
Materias:	cancer vaccines lewis lung carcinoma (llc) tumor-specific antigens testis antigens immunotherapy immune response Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/60aec8e12b0247809952f78a60b6773d
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spelling	oai:doaj.org-article:60aec8e12b0247809952f78a60b6773d2021-11-18T08:03:51ZEFFICIENCY OF THE FORMATION OF ANTITUMOR IMMUNE RESPONSES IN THE SYSTEM OF PREVENTIVE VACCINATION OF MICE WITH TESTICULAR ANTIGENS1563-06252313-741X10.15789/1563-0625-EOT-2299https://doaj.org/article/60aec8e12b0247809952f78a60b6773d2021-10-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/2299https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XАppearance of a malignant tumor is associated with impaired mechanisms of proliferation, differentiation, apoptosis ability. However, these changes are not enough for immune system to recognize and destroy mutated cells. Weak immunogenicity of tumor-associated antigens (TAA) and the insufficiency of co-stimulating molecules on the surface of tumor cells is a reason for this phenomenon Since biochemical processes of tumor cells and healthy tissue cells are identical, therefore creation of effective chemotherapeutic drugs is limited not by selectivity of their action. So antitumor vaccination is the most effective specific method for both preventing recurrence of a disease and a therapeutic treatment tool in oncology. Xenogeneic proteins are highly immunogenic and effective in breaking immune tolerance to human analogs. In our work, we used sheep testicular AG as a source xenogenic TAAs. Sheep testicles contain a large set of TAAs. Experimental mice were immunized with type liposomal testicular vaccine from sheep, one month after vaccination, to induce tumor growth, cells of carcinoma LLC were implanted in mouse. The life expectancy of the experimental group of mice was 2 times higher compared to the syngenetic control and 20% of them did not develop the tumor at all. In the spleen of mice who did not have tumors after pre-vaccination sheep liposomal testicular AG, T-regulatory cells and T-memory cells were measured. We found a credible decrease in both naive Treg (CD4+CD25+), activated (CD4+CD25+FoxP3+) and both T-memory (CD4+CD44+) and central memory (CD4+CD44+CD62L+) in spleen pre-vaccination mice with compared to the contral intact spleen. Content of IFNg and IL-10 in supernatants of mouse slenocytes derived from vaccinated mice with no tumors was investigated and showed a reliable decrease in the amount of IL-10, but not IFNg. We believe that immunization with xenogenic tumor AGs can lead to the formation of a protective antitumor response.A. B. DorzhievaT. S. KhabalovaYu. E. AndrosovaE. A. KaschenkoI. P. IvanovaG. V. SeledtsovaSPb RAACIarticlecancer vaccineslewis lung carcinoma (llc)tumor-specific antigenstestis antigensimmunotherapyimmune responseImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 23, Iss 4, Pp 665-670 (2021)
institution	DOAJ
collection	DOAJ
language	RU
topic	cancer vaccines lewis lung carcinoma (llc) tumor-specific antigens testis antigens immunotherapy immune response Immunologic diseases. Allergy RC581-607
spellingShingle	cancer vaccines lewis lung carcinoma (llc) tumor-specific antigens testis antigens immunotherapy immune response Immunologic diseases. Allergy RC581-607 A. B. Dorzhieva T. S. Khabalova Yu. E. Androsova E. A. Kaschenko I. P. Ivanova G. V. Seledtsova EFFICIENCY OF THE FORMATION OF ANTITUMOR IMMUNE RESPONSES IN THE SYSTEM OF PREVENTIVE VACCINATION OF MICE WITH TESTICULAR ANTIGENS
description	Аppearance of a malignant tumor is associated with impaired mechanisms of proliferation, differentiation, apoptosis ability. However, these changes are not enough for immune system to recognize and destroy mutated cells. Weak immunogenicity of tumor-associated antigens (TAA) and the insufficiency of co-stimulating molecules on the surface of tumor cells is a reason for this phenomenon Since biochemical processes of tumor cells and healthy tissue cells are identical, therefore creation of effective chemotherapeutic drugs is limited not by selectivity of their action. So antitumor vaccination is the most effective specific method for both preventing recurrence of a disease and a therapeutic treatment tool in oncology. Xenogeneic proteins are highly immunogenic and effective in breaking immune tolerance to human analogs. In our work, we used sheep testicular AG as a source xenogenic TAAs. Sheep testicles contain a large set of TAAs. Experimental mice were immunized with type liposomal testicular vaccine from sheep, one month after vaccination, to induce tumor growth, cells of carcinoma LLC were implanted in mouse. The life expectancy of the experimental group of mice was 2 times higher compared to the syngenetic control and 20% of them did not develop the tumor at all. In the spleen of mice who did not have tumors after pre-vaccination sheep liposomal testicular AG, T-regulatory cells and T-memory cells were measured. We found a credible decrease in both naive Treg (CD4+CD25+), activated (CD4+CD25+FoxP3+) and both T-memory (CD4+CD44+) and central memory (CD4+CD44+CD62L+) in spleen pre-vaccination mice with compared to the contral intact spleen. Content of IFNg and IL-10 in supernatants of mouse slenocytes derived from vaccinated mice with no tumors was investigated and showed a reliable decrease in the amount of IL-10, but not IFNg. We believe that immunization with xenogenic tumor AGs can lead to the formation of a protective antitumor response.
format	article
author	A. B. Dorzhieva T. S. Khabalova Yu. E. Androsova E. A. Kaschenko I. P. Ivanova G. V. Seledtsova
author_facet	A. B. Dorzhieva T. S. Khabalova Yu. E. Androsova E. A. Kaschenko I. P. Ivanova G. V. Seledtsova
author_sort	A. B. Dorzhieva
title	EFFICIENCY OF THE FORMATION OF ANTITUMOR IMMUNE RESPONSES IN THE SYSTEM OF PREVENTIVE VACCINATION OF MICE WITH TESTICULAR ANTIGENS
title_short	EFFICIENCY OF THE FORMATION OF ANTITUMOR IMMUNE RESPONSES IN THE SYSTEM OF PREVENTIVE VACCINATION OF MICE WITH TESTICULAR ANTIGENS
title_full	EFFICIENCY OF THE FORMATION OF ANTITUMOR IMMUNE RESPONSES IN THE SYSTEM OF PREVENTIVE VACCINATION OF MICE WITH TESTICULAR ANTIGENS
title_fullStr	EFFICIENCY OF THE FORMATION OF ANTITUMOR IMMUNE RESPONSES IN THE SYSTEM OF PREVENTIVE VACCINATION OF MICE WITH TESTICULAR ANTIGENS
title_full_unstemmed	EFFICIENCY OF THE FORMATION OF ANTITUMOR IMMUNE RESPONSES IN THE SYSTEM OF PREVENTIVE VACCINATION OF MICE WITH TESTICULAR ANTIGENS
title_sort	efficiency of the formation of antitumor immune responses in the system of preventive vaccination of mice with testicular antigens
publisher	SPb RAACI
publishDate	2021
url	https://doaj.org/article/60aec8e12b0247809952f78a60b6773d
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EFFICIENCY OF THE FORMATION OF ANTITUMOR IMMUNE RESPONSES IN THE SYSTEM OF PREVENTIVE VACCINATION OF MICE WITH TESTICULAR ANTIGENS

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