Molecular characterization of four giant gourami strains from Java and Sumatra

Nuryanto A, Amalia G, Khairani D, Pramono H, Bhagawati D. 2018. Molecular characterization of four giant gourami strains from Java and Sumatra. Biodiversitas 19: 578-584. Giant gourami (Osprhonemus goramy) are widely distibuted across Indonesia, such Java, Sumatra, and Kalimatan which lead to the em...

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Autores principales:	AGUS NURYANTO, GINA AMALIA, DAINTY KHAIRANI, HENDRO PRAMONO, DIAN BHAGAWATI
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2018
Materias:	fixation index gourami java molecular divergence osphronemus goramy sumatra Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/60b5cbe266c543eeb3da650ec3245b3d
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spelling	oai:doaj.org-article:60b5cbe266c543eeb3da650ec3245b3d2021-11-16T14:01:41ZMolecular characterization of four giant gourami strains from Java and Sumatra1412-033X2085-472210.13057/biodiv/d190228https://doaj.org/article/60b5cbe266c543eeb3da650ec3245b3d2018-03-01T00:00:00Zhttps://smujo.id/biodiv/article/view/2540https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Nuryanto A, Amalia G, Khairani D, Pramono H, Bhagawati D. 2018. Molecular characterization of four giant gourami strains from Java and Sumatra. Biodiversitas 19: 578-584. Giant gourami (Osprhonemus goramy) are widely distibuted across Indonesia, such Java, Sumatra, and Kalimatan which lead to the emerge of various gourami strains due to morphological differences. However, no scientific data about the relationship between morphological and genetic differences among strains. This research aimed to obtain information on molecular characteristics of four giant gourami strains from Java and Sumatra based on partial sequences of cytochrome c oxidase 1 gene. This information is vital to strengthen their taxonomic status. Caudal fin clips were sampled from each strain. Nucleotide sequencing was performed using bigdye terminator tehcnique. Pairwise Fst comparison was carried out using arlequin software, whereas sequences of divergence analysis was performed in DnaSP software. Homology of the sequences were checked with previous published data available in Boldsystem data base. Homology test resulted in 98.79 to 100% similarity to the previous published sequences. This means that all strains belonged to single species, i.e. Osphronemus goramy. This placement was supported by low-level of genetic divergences among strains. Although they have low-level genetic divergences, this value is suitable to separate each strain clearly as indicated by pairwise Fst comparison analysis and AMOVA, which showed differences among strains. However, phylogenetic tree shows that all stains formed a monopyletic group with boostratp value of 100. Phylogenetic analysis supports the placement of all strains into a single species that is O. guramy. Those morphological differences are also reflected in their genetic character, except for Tambago and Oranye strains.AGUS NURYANTOGINA AMALIADAINTY KHAIRANIHENDRO PRAMONODIAN BHAGAWATIMBI & UNS Soloarticlefixation indexgouramijavamolecular divergenceosphronemus goramysumatraBiology (General)QH301-705.5ENBiodiversitas, Vol 19, Iss 2, Pp 528-534 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	fixation index gourami java molecular divergence osphronemus goramy sumatra Biology (General) QH301-705.5
spellingShingle	fixation index gourami java molecular divergence osphronemus goramy sumatra Biology (General) QH301-705.5 AGUS NURYANTO GINA AMALIA DAINTY KHAIRANI HENDRO PRAMONO DIAN BHAGAWATI Molecular characterization of four giant gourami strains from Java and Sumatra
description	Nuryanto A, Amalia G, Khairani D, Pramono H, Bhagawati D. 2018. Molecular characterization of four giant gourami strains from Java and Sumatra. Biodiversitas 19: 578-584. Giant gourami (Osprhonemus goramy) are widely distibuted across Indonesia, such Java, Sumatra, and Kalimatan which lead to the emerge of various gourami strains due to morphological differences. However, no scientific data about the relationship between morphological and genetic differences among strains. This research aimed to obtain information on molecular characteristics of four giant gourami strains from Java and Sumatra based on partial sequences of cytochrome c oxidase 1 gene. This information is vital to strengthen their taxonomic status. Caudal fin clips were sampled from each strain. Nucleotide sequencing was performed using bigdye terminator tehcnique. Pairwise Fst comparison was carried out using arlequin software, whereas sequences of divergence analysis was performed in DnaSP software. Homology of the sequences were checked with previous published data available in Boldsystem data base. Homology test resulted in 98.79 to 100% similarity to the previous published sequences. This means that all strains belonged to single species, i.e. Osphronemus goramy. This placement was supported by low-level of genetic divergences among strains. Although they have low-level genetic divergences, this value is suitable to separate each strain clearly as indicated by pairwise Fst comparison analysis and AMOVA, which showed differences among strains. However, phylogenetic tree shows that all stains formed a monopyletic group with boostratp value of 100. Phylogenetic analysis supports the placement of all strains into a single species that is O. guramy. Those morphological differences are also reflected in their genetic character, except for Tambago and Oranye strains.
format	article
author	AGUS NURYANTO GINA AMALIA DAINTY KHAIRANI HENDRO PRAMONO DIAN BHAGAWATI
author_facet	AGUS NURYANTO GINA AMALIA DAINTY KHAIRANI HENDRO PRAMONO DIAN BHAGAWATI
author_sort	AGUS NURYANTO
title	Molecular characterization of four giant gourami strains from Java and Sumatra
title_short	Molecular characterization of four giant gourami strains from Java and Sumatra
title_full	Molecular characterization of four giant gourami strains from Java and Sumatra
title_fullStr	Molecular characterization of four giant gourami strains from Java and Sumatra
title_full_unstemmed	Molecular characterization of four giant gourami strains from Java and Sumatra
title_sort	molecular characterization of four giant gourami strains from java and sumatra
publisher	MBI & UNS Solo
publishDate	2018
url	https://doaj.org/article/60b5cbe266c543eeb3da650ec3245b3d
work_keys_str_mv	AT agusnuryanto molecularcharacterizationoffourgiantgouramistrainsfromjavaandsumatra AT ginaamalia molecularcharacterizationoffourgiantgouramistrainsfromjavaandsumatra AT daintykhairani molecularcharacterizationoffourgiantgouramistrainsfromjavaandsumatra AT hendropramono molecularcharacterizationoffourgiantgouramistrainsfromjavaandsumatra AT dianbhagawati molecularcharacterizationoffourgiantgouramistrainsfromjavaandsumatra
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Molecular characterization of four giant gourami strains from Java and Sumatra

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