Decellularised skeletal muscles allow functional muscle regeneration by promoting host cell migration

Abstract Pathological conditions affecting skeletal muscle function may lead to irreversible volumetric muscle loss (VML). Therapeutic approaches involving acellular matrices represent an emerging and promising strategy to promote regeneration of skeletal muscle following injury. Here we investigate...

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Autores principales:	Anna Urciuolo, Luca Urbani, Silvia Perin, Panagiotis Maghsoudlou, Federico Scottoni, Asllan Gjinovci, Henry Collins-Hooper, Stavros Loukogeorgakis, Athanasios Tyraskis, Silvia Torelli, Elena Germinario, Mario Enrique Alvarez Fallas, Carla Julia-Vilella, Simon Eaton, Bert Blaauw, Ketan Patel, Paolo De Coppi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/60c000f05b17423389276b399e8a2068
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spelling	oai:doaj.org-article:60c000f05b17423389276b399e8a20682021-12-02T15:08:29ZDecellularised skeletal muscles allow functional muscle regeneration by promoting host cell migration10.1038/s41598-018-26371-y2045-2322https://doaj.org/article/60c000f05b17423389276b399e8a20682018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-26371-yhttps://doaj.org/toc/2045-2322Abstract Pathological conditions affecting skeletal muscle function may lead to irreversible volumetric muscle loss (VML). Therapeutic approaches involving acellular matrices represent an emerging and promising strategy to promote regeneration of skeletal muscle following injury. Here we investigated the ability of three different decellularised skeletal muscle scaffolds to support muscle regeneration in a xenogeneic immune-competent model of VML, in which the EDL muscle was surgically resected. All implanted acellular matrices, used to replace the resected muscles, were able to generate functional artificial muscles by promoting host myogenic cell migration and differentiation, as well as nervous fibres, vascular networks, and satellite cell (SC) homing. However, acellular tissue mainly composed of extracellular matrix (ECM) allowed better myofibre three-dimensional (3D) organization and the restoration of SC pool, when compared to scaffolds which also preserved muscular cytoskeletal structures. Finally, we showed that fibroblasts are indispensable to promote efficient migration and myogenesis by muscle stem cells across the scaffolds in vitro. This data strongly support the use of xenogeneic acellular muscles as device to treat VML conditions in absence of donor cell implementation, as well as in vitro model for studying cell interplay during myogenesis.Anna UrciuoloLuca UrbaniSilvia PerinPanagiotis MaghsoudlouFederico ScottoniAsllan GjinovciHenry Collins-HooperStavros LoukogeorgakisAthanasios TyraskisSilvia TorelliElena GerminarioMario Enrique Alvarez FallasCarla Julia-VilellaSimon EatonBert BlaauwKetan PatelPaolo De CoppiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-20 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anna Urciuolo Luca Urbani Silvia Perin Panagiotis Maghsoudlou Federico Scottoni Asllan Gjinovci Henry Collins-Hooper Stavros Loukogeorgakis Athanasios Tyraskis Silvia Torelli Elena Germinario Mario Enrique Alvarez Fallas Carla Julia-Vilella Simon Eaton Bert Blaauw Ketan Patel Paolo De Coppi Decellularised skeletal muscles allow functional muscle regeneration by promoting host cell migration
description	Abstract Pathological conditions affecting skeletal muscle function may lead to irreversible volumetric muscle loss (VML). Therapeutic approaches involving acellular matrices represent an emerging and promising strategy to promote regeneration of skeletal muscle following injury. Here we investigated the ability of three different decellularised skeletal muscle scaffolds to support muscle regeneration in a xenogeneic immune-competent model of VML, in which the EDL muscle was surgically resected. All implanted acellular matrices, used to replace the resected muscles, were able to generate functional artificial muscles by promoting host myogenic cell migration and differentiation, as well as nervous fibres, vascular networks, and satellite cell (SC) homing. However, acellular tissue mainly composed of extracellular matrix (ECM) allowed better myofibre three-dimensional (3D) organization and the restoration of SC pool, when compared to scaffolds which also preserved muscular cytoskeletal structures. Finally, we showed that fibroblasts are indispensable to promote efficient migration and myogenesis by muscle stem cells across the scaffolds in vitro. This data strongly support the use of xenogeneic acellular muscles as device to treat VML conditions in absence of donor cell implementation, as well as in vitro model for studying cell interplay during myogenesis.
format	article
author	Anna Urciuolo Luca Urbani Silvia Perin Panagiotis Maghsoudlou Federico Scottoni Asllan Gjinovci Henry Collins-Hooper Stavros Loukogeorgakis Athanasios Tyraskis Silvia Torelli Elena Germinario Mario Enrique Alvarez Fallas Carla Julia-Vilella Simon Eaton Bert Blaauw Ketan Patel Paolo De Coppi
author_facet	Anna Urciuolo Luca Urbani Silvia Perin Panagiotis Maghsoudlou Federico Scottoni Asllan Gjinovci Henry Collins-Hooper Stavros Loukogeorgakis Athanasios Tyraskis Silvia Torelli Elena Germinario Mario Enrique Alvarez Fallas Carla Julia-Vilella Simon Eaton Bert Blaauw Ketan Patel Paolo De Coppi
author_sort	Anna Urciuolo
title	Decellularised skeletal muscles allow functional muscle regeneration by promoting host cell migration
title_short	Decellularised skeletal muscles allow functional muscle regeneration by promoting host cell migration
title_full	Decellularised skeletal muscles allow functional muscle regeneration by promoting host cell migration
title_fullStr	Decellularised skeletal muscles allow functional muscle regeneration by promoting host cell migration
title_full_unstemmed	Decellularised skeletal muscles allow functional muscle regeneration by promoting host cell migration
title_sort	decellularised skeletal muscles allow functional muscle regeneration by promoting host cell migration
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/60c000f05b17423389276b399e8a2068
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Decellularised skeletal muscles allow functional muscle regeneration by promoting host cell migration

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