Prevalence of transmitted HIV drug resistance in Iran between 2010 and 2011.
<h4>Objective</h4>Drug-resistant (DR) HIV emerges during combined antiretroviral treatment (cART), creating concern about widespread transmission of DR-HIV as cART is expanded in resource-limited countries. The aim of this study was to determine the predominant HIV-1 subtypes and prevale...
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2013
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oai:doaj.org-article:60c3629b7fe94aae8b6a18e002068b592021-11-18T07:48:23ZPrevalence of transmitted HIV drug resistance in Iran between 2010 and 2011.1932-620310.1371/journal.pone.0061864https://doaj.org/article/60c3629b7fe94aae8b6a18e002068b592013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23626742/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Objective</h4>Drug-resistant (DR) HIV emerges during combined antiretroviral treatment (cART), creating concern about widespread transmission of DR-HIV as cART is expanded in resource-limited countries. The aim of this study was to determine the predominant HIV-1 subtypes and prevalence of transmitted DR mutations among antiretroviral-naïve patients in Iran.<h4>Design</h4>To monitor transmission of DR HIV, a threshold surveillance based on the world health organization (WHO) guidelines was implemented in Iran.<h4>Methods</h4>For this HIVDR threshold surveillance study, blood samples were collected from 50 antiretroviral-naïve HIV-1-infected patients. Antiretroviral-resistant mutations were determined by sequencing HIV-1 protease, reverse transcriptase and integrase regions. The HIV-1 subtype was determined by sequencing the p17 and C2-V5 regions of the gag and env genes, respectively.<h4>Results</h4>Phylogenetic analyses of the sequenced regions revealed that 45 (95.7%) of 47 samples that were successfully obtained were CRF35_AD. The remaining two cases were subtype B (2.1%) and CRF01_AE (2.1%). Consistent results were obtained also from Env and Gag sequences. Regarding prevalence of transmitted DR viruses, two cases were found to harbor reverse transcriptase-inhibitor-resistant mutations (4.3%). In addition, although not in the WHO list for surveillance of transmitted mutations, 13 minor protease-inhibitor-resistant mutations listed in the International AIDS Society-USA panel of drug resistance mutations were found. No DR mutations were detected in the integrase region.<h4>Conclusions</h4>Our study clarified that CRF35_AD is the major subtype among HIV-1-infected patients in Iran. According to the WHO categorization method of HIVDR threshold survey, the prevalence of transmitted drug resistant HIV in Iran was estimated as moderate (5-15%).Fatemeh JahanbakhshJunko HattoriMasakazu MatsudaShiro IbeSeyed-Hamid R MonavariArash MemarnejadianMohammad R AghasadeghiEhsan MostafaviMinoo MohrazHossain JabbariKianoush KamaliHossein KeyvaniKayhan AzadmaneshWataru SugiuraPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 4, p e61864 (2013) |
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Medicine R Science Q Fatemeh Jahanbakhsh Junko Hattori Masakazu Matsuda Shiro Ibe Seyed-Hamid R Monavari Arash Memarnejadian Mohammad R Aghasadeghi Ehsan Mostafavi Minoo Mohraz Hossain Jabbari Kianoush Kamali Hossein Keyvani Kayhan Azadmanesh Wataru Sugiura Prevalence of transmitted HIV drug resistance in Iran between 2010 and 2011. |
| description |
<h4>Objective</h4>Drug-resistant (DR) HIV emerges during combined antiretroviral treatment (cART), creating concern about widespread transmission of DR-HIV as cART is expanded in resource-limited countries. The aim of this study was to determine the predominant HIV-1 subtypes and prevalence of transmitted DR mutations among antiretroviral-naïve patients in Iran.<h4>Design</h4>To monitor transmission of DR HIV, a threshold surveillance based on the world health organization (WHO) guidelines was implemented in Iran.<h4>Methods</h4>For this HIVDR threshold surveillance study, blood samples were collected from 50 antiretroviral-naïve HIV-1-infected patients. Antiretroviral-resistant mutations were determined by sequencing HIV-1 protease, reverse transcriptase and integrase regions. The HIV-1 subtype was determined by sequencing the p17 and C2-V5 regions of the gag and env genes, respectively.<h4>Results</h4>Phylogenetic analyses of the sequenced regions revealed that 45 (95.7%) of 47 samples that were successfully obtained were CRF35_AD. The remaining two cases were subtype B (2.1%) and CRF01_AE (2.1%). Consistent results were obtained also from Env and Gag sequences. Regarding prevalence of transmitted DR viruses, two cases were found to harbor reverse transcriptase-inhibitor-resistant mutations (4.3%). In addition, although not in the WHO list for surveillance of transmitted mutations, 13 minor protease-inhibitor-resistant mutations listed in the International AIDS Society-USA panel of drug resistance mutations were found. No DR mutations were detected in the integrase region.<h4>Conclusions</h4>Our study clarified that CRF35_AD is the major subtype among HIV-1-infected patients in Iran. According to the WHO categorization method of HIVDR threshold survey, the prevalence of transmitted drug resistant HIV in Iran was estimated as moderate (5-15%). |
| format |
article |
| author |
Fatemeh Jahanbakhsh Junko Hattori Masakazu Matsuda Shiro Ibe Seyed-Hamid R Monavari Arash Memarnejadian Mohammad R Aghasadeghi Ehsan Mostafavi Minoo Mohraz Hossain Jabbari Kianoush Kamali Hossein Keyvani Kayhan Azadmanesh Wataru Sugiura |
| author_facet |
Fatemeh Jahanbakhsh Junko Hattori Masakazu Matsuda Shiro Ibe Seyed-Hamid R Monavari Arash Memarnejadian Mohammad R Aghasadeghi Ehsan Mostafavi Minoo Mohraz Hossain Jabbari Kianoush Kamali Hossein Keyvani Kayhan Azadmanesh Wataru Sugiura |
| author_sort |
Fatemeh Jahanbakhsh |
| title |
Prevalence of transmitted HIV drug resistance in Iran between 2010 and 2011. |
| title_short |
Prevalence of transmitted HIV drug resistance in Iran between 2010 and 2011. |
| title_full |
Prevalence of transmitted HIV drug resistance in Iran between 2010 and 2011. |
| title_fullStr |
Prevalence of transmitted HIV drug resistance in Iran between 2010 and 2011. |
| title_full_unstemmed |
Prevalence of transmitted HIV drug resistance in Iran between 2010 and 2011. |
| title_sort |
prevalence of transmitted hiv drug resistance in iran between 2010 and 2011. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2013 |
| url |
https://doaj.org/article/60c3629b7fe94aae8b6a18e002068b59 |
| work_keys_str_mv |
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