Mesenchymal stromal cells in the bone marrow niche consist of multi-populations with distinct transcriptional and epigenetic properties

Abstract Although multiple studies have investigated the mesenchymal stem and progenitor cells (MSCs) that give rise to mature bone marrow, high heterogeneity in their morphologies and properties causes difficulties in molecular separation of their distinct populations. In this study, by taking adva...

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Autores principales:	Sanshiro Kanazawa, Hiroyuki Okada, Hironori Hojo, Shinsuke Ohba, Junichi Iwata, Makoto Komura, Atsuhiko Hikita, Kazuto Hoshi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/60c5606e53ea40dab28e063d2783bad7
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spelling	oai:doaj.org-article:60c5606e53ea40dab28e063d2783bad72021-12-02T18:49:28ZMesenchymal stromal cells in the bone marrow niche consist of multi-populations with distinct transcriptional and epigenetic properties10.1038/s41598-021-94186-52045-2322https://doaj.org/article/60c5606e53ea40dab28e063d2783bad72021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94186-5https://doaj.org/toc/2045-2322Abstract Although multiple studies have investigated the mesenchymal stem and progenitor cells (MSCs) that give rise to mature bone marrow, high heterogeneity in their morphologies and properties causes difficulties in molecular separation of their distinct populations. In this study, by taking advantage of the resolution of the single cell transcriptome, we analyzed Sca-1 and PDGFR-α fraction in the mouse bone marrow tissue. The single cell transcriptome enabled us to further classify the population into seven populations according to their gene expression profiles. We then separately obtained the seven populations based on candidate marker genes, and specified their gene expression properties and epigenetic landscape by ATAC-seq. Our findings will enable to elucidate the stem cell niche signal in the bone marrow microenvironment, reconstitute bone marrow in vitro, and shed light on the potentially important role of identified subpopulation in various clinical applications to the treatment of bone- and bone marrow-related diseases.Sanshiro KanazawaHiroyuki OkadaHironori HojoShinsuke OhbaJunichi IwataMakoto KomuraAtsuhiko HikitaKazuto HoshiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sanshiro Kanazawa Hiroyuki Okada Hironori Hojo Shinsuke Ohba Junichi Iwata Makoto Komura Atsuhiko Hikita Kazuto Hoshi Mesenchymal stromal cells in the bone marrow niche consist of multi-populations with distinct transcriptional and epigenetic properties
description	Abstract Although multiple studies have investigated the mesenchymal stem and progenitor cells (MSCs) that give rise to mature bone marrow, high heterogeneity in their morphologies and properties causes difficulties in molecular separation of their distinct populations. In this study, by taking advantage of the resolution of the single cell transcriptome, we analyzed Sca-1 and PDGFR-α fraction in the mouse bone marrow tissue. The single cell transcriptome enabled us to further classify the population into seven populations according to their gene expression profiles. We then separately obtained the seven populations based on candidate marker genes, and specified their gene expression properties and epigenetic landscape by ATAC-seq. Our findings will enable to elucidate the stem cell niche signal in the bone marrow microenvironment, reconstitute bone marrow in vitro, and shed light on the potentially important role of identified subpopulation in various clinical applications to the treatment of bone- and bone marrow-related diseases.
format	article
author	Sanshiro Kanazawa Hiroyuki Okada Hironori Hojo Shinsuke Ohba Junichi Iwata Makoto Komura Atsuhiko Hikita Kazuto Hoshi
author_facet	Sanshiro Kanazawa Hiroyuki Okada Hironori Hojo Shinsuke Ohba Junichi Iwata Makoto Komura Atsuhiko Hikita Kazuto Hoshi
author_sort	Sanshiro Kanazawa
title	Mesenchymal stromal cells in the bone marrow niche consist of multi-populations with distinct transcriptional and epigenetic properties
title_short	Mesenchymal stromal cells in the bone marrow niche consist of multi-populations with distinct transcriptional and epigenetic properties
title_full	Mesenchymal stromal cells in the bone marrow niche consist of multi-populations with distinct transcriptional and epigenetic properties
title_fullStr	Mesenchymal stromal cells in the bone marrow niche consist of multi-populations with distinct transcriptional and epigenetic properties
title_full_unstemmed	Mesenchymal stromal cells in the bone marrow niche consist of multi-populations with distinct transcriptional and epigenetic properties
title_sort	mesenchymal stromal cells in the bone marrow niche consist of multi-populations with distinct transcriptional and epigenetic properties
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/60c5606e53ea40dab28e063d2783bad7
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Mesenchymal stromal cells in the bone marrow niche consist of multi-populations with distinct transcriptional and epigenetic properties

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