Wei Chang An pill regulates gastrointestinal motility in a bidirectional manner

Context Wei Chang An (WCA) is a commercial prescription developed for the coordination of gastrointestinal movement. Objective To investigate the role of WCA in the regulation of diarrhoea and constipation in rats. Material and methods The diarrhoea and constipation models were prepared by gavage of...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sitong Jia, Lijuan Chai, Jing Zhang, Min Zhang, Lin Li, Yaxin Qi, Yafen Pang, Xi Chen, Nana Fan, Lin Wang, Yujing Wang, Jixiang Song, Yingjie Sun, Yi Wang, Lin Miao, Han Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	diarrhoea constipation rho-associated coiled-coil forming protein kinase-1 (rock-1) myosin light chain kinase (mlck) irritable bowel syndrome (ibs) Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/60c5b5ebf3fc429f9e2b6bab77ed32ff
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:60c5b5ebf3fc429f9e2b6bab77ed32ff
record_format	dspace
spelling	oai:doaj.org-article:60c5b5ebf3fc429f9e2b6bab77ed32ff2021-11-04T15:00:41ZWei Chang An pill regulates gastrointestinal motility in a bidirectional manner1388-02091744-511610.1080/13880209.2021.1991383https://doaj.org/article/60c5b5ebf3fc429f9e2b6bab77ed32ff2021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/13880209.2021.1991383https://doaj.org/toc/1388-0209https://doaj.org/toc/1744-5116Context Wei Chang An (WCA) is a commercial prescription developed for the coordination of gastrointestinal movement. Objective To investigate the role of WCA in the regulation of diarrhoea and constipation in rats. Material and methods The diarrhoea and constipation models were prepared by gavage of Folium senna and diphenoxylate hydrochloride. Rats were randomized equally (n = 6) into the normal group given saline daily, the positive group given Pinaverium Bromide (13.5 mg/kg) or Sennoside A (0.1 mg/kg) and three WCA-treated groups (22, 44, and 88 mg/kg) by gavage daily for 7 consecutive days. The effects of WCA were assessed by a series of faecal symptoms and histopathology. Gastrointestinal parameters were determined by ELISA. The effect of WCA on gastrointestinal tissues was evaluated by strip assay. Expression of ROCK-1 and MLCK was measured by RT-PCR and Western blotting. Results Data from Bristol stool form scale, diarrhoea index, visceral sensitivity, defaecation time, and intestinal propulsive rate showed that WCA protected rats against diarrhoea and constipation (p < 0.01). The up-regulation of Substance P and 5-hydroxytryptamine in diarrhoea rats and down-regulation of Substance P and vasoactive intestinal polypeptide in constipation rats were inhibited by WCA (p < 0.05). WCA stimulated the gastrointestinal strip contractions but inhibited ACh-induced contractions (p < 0.01). The decreased ROCK-1 and MLCK expression in diarrhoea rats and increased in constipation rats were suppressed by WCA (p < 0.01). Conclusions WCA has both antidiarrhea and anti-constipation effects, suggesting its bidirectional role in gastrointestinal modulation, and providing evidence of WCA for irritable bowel syndrome treatment.Sitong JiaLijuan ChaiJing ZhangMin ZhangLin LiYaxin QiYafen PangXi ChenNana FanLin WangYujing WangJixiang SongYingjie SunYi WangLin MiaoHan ZhangTaylor & Francis Grouparticlediarrhoeaconstipationrho-associated coiled-coil forming protein kinase-1 (rock-1)myosin light chain kinase (mlck)irritable bowel syndrome (ibs)Therapeutics. PharmacologyRM1-950ENPharmaceutical Biology, Vol 59, Iss 1, Pp 1452-1463 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	diarrhoea constipation rho-associated coiled-coil forming protein kinase-1 (rock-1) myosin light chain kinase (mlck) irritable bowel syndrome (ibs) Therapeutics. Pharmacology RM1-950
spellingShingle	diarrhoea constipation rho-associated coiled-coil forming protein kinase-1 (rock-1) myosin light chain kinase (mlck) irritable bowel syndrome (ibs) Therapeutics. Pharmacology RM1-950 Sitong Jia Lijuan Chai Jing Zhang Min Zhang Lin Li Yaxin Qi Yafen Pang Xi Chen Nana Fan Lin Wang Yujing Wang Jixiang Song Yingjie Sun Yi Wang Lin Miao Han Zhang Wei Chang An pill regulates gastrointestinal motility in a bidirectional manner
description	Context Wei Chang An (WCA) is a commercial prescription developed for the coordination of gastrointestinal movement. Objective To investigate the role of WCA in the regulation of diarrhoea and constipation in rats. Material and methods The diarrhoea and constipation models were prepared by gavage of Folium senna and diphenoxylate hydrochloride. Rats were randomized equally (n = 6) into the normal group given saline daily, the positive group given Pinaverium Bromide (13.5 mg/kg) or Sennoside A (0.1 mg/kg) and three WCA-treated groups (22, 44, and 88 mg/kg) by gavage daily for 7 consecutive days. The effects of WCA were assessed by a series of faecal symptoms and histopathology. Gastrointestinal parameters were determined by ELISA. The effect of WCA on gastrointestinal tissues was evaluated by strip assay. Expression of ROCK-1 and MLCK was measured by RT-PCR and Western blotting. Results Data from Bristol stool form scale, diarrhoea index, visceral sensitivity, defaecation time, and intestinal propulsive rate showed that WCA protected rats against diarrhoea and constipation (p < 0.01). The up-regulation of Substance P and 5-hydroxytryptamine in diarrhoea rats and down-regulation of Substance P and vasoactive intestinal polypeptide in constipation rats were inhibited by WCA (p < 0.05). WCA stimulated the gastrointestinal strip contractions but inhibited ACh-induced contractions (p < 0.01). The decreased ROCK-1 and MLCK expression in diarrhoea rats and increased in constipation rats were suppressed by WCA (p < 0.01). Conclusions WCA has both antidiarrhea and anti-constipation effects, suggesting its bidirectional role in gastrointestinal modulation, and providing evidence of WCA for irritable bowel syndrome treatment.
format	article
author	Sitong Jia Lijuan Chai Jing Zhang Min Zhang Lin Li Yaxin Qi Yafen Pang Xi Chen Nana Fan Lin Wang Yujing Wang Jixiang Song Yingjie Sun Yi Wang Lin Miao Han Zhang
author_facet	Sitong Jia Lijuan Chai Jing Zhang Min Zhang Lin Li Yaxin Qi Yafen Pang Xi Chen Nana Fan Lin Wang Yujing Wang Jixiang Song Yingjie Sun Yi Wang Lin Miao Han Zhang
author_sort	Sitong Jia
title	Wei Chang An pill regulates gastrointestinal motility in a bidirectional manner
title_short	Wei Chang An pill regulates gastrointestinal motility in a bidirectional manner
title_full	Wei Chang An pill regulates gastrointestinal motility in a bidirectional manner
title_fullStr	Wei Chang An pill regulates gastrointestinal motility in a bidirectional manner
title_full_unstemmed	Wei Chang An pill regulates gastrointestinal motility in a bidirectional manner
title_sort	wei chang an pill regulates gastrointestinal motility in a bidirectional manner
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/60c5b5ebf3fc429f9e2b6bab77ed32ff
work_keys_str_mv	AT sitongjia weichanganpillregulatesgastrointestinalmotilityinabidirectionalmanner AT lijuanchai weichanganpillregulatesgastrointestinalmotilityinabidirectionalmanner AT jingzhang weichanganpillregulatesgastrointestinalmotilityinabidirectionalmanner AT minzhang weichanganpillregulatesgastrointestinalmotilityinabidirectionalmanner AT linli weichanganpillregulatesgastrointestinalmotilityinabidirectionalmanner AT yaxinqi weichanganpillregulatesgastrointestinalmotilityinabidirectionalmanner AT yafenpang weichanganpillregulatesgastrointestinalmotilityinabidirectionalmanner AT xichen weichanganpillregulatesgastrointestinalmotilityinabidirectionalmanner AT nanafan weichanganpillregulatesgastrointestinalmotilityinabidirectionalmanner AT linwang weichanganpillregulatesgastrointestinalmotilityinabidirectionalmanner AT yujingwang weichanganpillregulatesgastrointestinalmotilityinabidirectionalmanner AT jixiangsong weichanganpillregulatesgastrointestinalmotilityinabidirectionalmanner AT yingjiesun weichanganpillregulatesgastrointestinalmotilityinabidirectionalmanner AT yiwang weichanganpillregulatesgastrointestinalmotilityinabidirectionalmanner AT linmiao weichanganpillregulatesgastrointestinalmotilityinabidirectionalmanner AT hanzhang weichanganpillregulatesgastrointestinalmotilityinabidirectionalmanner
_version_	1718444773827674112

Wei Chang An pill regulates gastrointestinal motility in a bidirectional manner

Ejemplares similares