Dynamic swimming pattern of Pseudomonas aeruginosa near a vertical wall during initial attachment stages of biofilm formation

Abstract Studying the swimming behaviour of bacteria in 3 dimensions (3D) allows us to understand critical biological processes, such as biofilm formation. It is still unclear how near wall swimming behaviour may regulate the initial attachment and biofilm formation. It is challenging to address thi...

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Autores principales:	Nicole Zi-Jia Khong, Yukai Zeng, Soak-Kuan Lai, Cheng-Gee Koh, Zhao-Xun Liang, Keng-Hwee Chiam, Hoi-Yeung Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/60cb3094c858476a99b1f4a0c3b6f43b
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spelling	oai:doaj.org-article:60cb3094c858476a99b1f4a0c3b6f43b2021-12-02T13:50:42ZDynamic swimming pattern of Pseudomonas aeruginosa near a vertical wall during initial attachment stages of biofilm formation10.1038/s41598-021-81621-w2045-2322https://doaj.org/article/60cb3094c858476a99b1f4a0c3b6f43b2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81621-whttps://doaj.org/toc/2045-2322Abstract Studying the swimming behaviour of bacteria in 3 dimensions (3D) allows us to understand critical biological processes, such as biofilm formation. It is still unclear how near wall swimming behaviour may regulate the initial attachment and biofilm formation. It is challenging to address this as visualizing the movement of bacteria with reasonable spatial and temporal resolution in a high-throughput manner is technically difficult. Here, we compared the near wall (vertical) swimming behaviour of P. aeruginosa (PAO1) and its mutants ΔdipA (reduced in swarming motility and increased in biofilm formation) and ΔfimX (deficient in twitching motility and reduced in biofilm formation) using our new imaging technique based on light sheet microscopy. We found that P. aeruginosa (PAO1) increases its speed and changes its swimming angle drastically when it gets closer to a wall. In contrast, ΔdipA mutant moves toward the wall with steady speed without changing of swimming angle. The near wall behavior of ΔdipA allows it to be more effective to interact with the wall or wall-attached cells, thus leading to more adhesion events and a larger biofilm volume during initial attachment when compared with PAO1. Furthermore, we found that ΔfimX has a similar near wall swimming behavior as PAO1. However, it has a higher dispersal frequency and smaller biofilm formation when compared with PAO1 which can be explained by its poor twitching motility. Together, we propose that near wall swimming behavior of P. aeruginosa plays an important role in the regulation of initial attachment and biofilm formation.Nicole Zi-Jia KhongYukai ZengSoak-Kuan LaiCheng-Gee KohZhao-Xun LiangKeng-Hwee ChiamHoi-Yeung LiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nicole Zi-Jia Khong Yukai Zeng Soak-Kuan Lai Cheng-Gee Koh Zhao-Xun Liang Keng-Hwee Chiam Hoi-Yeung Li Dynamic swimming pattern of Pseudomonas aeruginosa near a vertical wall during initial attachment stages of biofilm formation
description	Abstract Studying the swimming behaviour of bacteria in 3 dimensions (3D) allows us to understand critical biological processes, such as biofilm formation. It is still unclear how near wall swimming behaviour may regulate the initial attachment and biofilm formation. It is challenging to address this as visualizing the movement of bacteria with reasonable spatial and temporal resolution in a high-throughput manner is technically difficult. Here, we compared the near wall (vertical) swimming behaviour of P. aeruginosa (PAO1) and its mutants ΔdipA (reduced in swarming motility and increased in biofilm formation) and ΔfimX (deficient in twitching motility and reduced in biofilm formation) using our new imaging technique based on light sheet microscopy. We found that P. aeruginosa (PAO1) increases its speed and changes its swimming angle drastically when it gets closer to a wall. In contrast, ΔdipA mutant moves toward the wall with steady speed without changing of swimming angle. The near wall behavior of ΔdipA allows it to be more effective to interact with the wall or wall-attached cells, thus leading to more adhesion events and a larger biofilm volume during initial attachment when compared with PAO1. Furthermore, we found that ΔfimX has a similar near wall swimming behavior as PAO1. However, it has a higher dispersal frequency and smaller biofilm formation when compared with PAO1 which can be explained by its poor twitching motility. Together, we propose that near wall swimming behavior of P. aeruginosa plays an important role in the regulation of initial attachment and biofilm formation.
format	article
author	Nicole Zi-Jia Khong Yukai Zeng Soak-Kuan Lai Cheng-Gee Koh Zhao-Xun Liang Keng-Hwee Chiam Hoi-Yeung Li
author_facet	Nicole Zi-Jia Khong Yukai Zeng Soak-Kuan Lai Cheng-Gee Koh Zhao-Xun Liang Keng-Hwee Chiam Hoi-Yeung Li
author_sort	Nicole Zi-Jia Khong
title	Dynamic swimming pattern of Pseudomonas aeruginosa near a vertical wall during initial attachment stages of biofilm formation
title_short	Dynamic swimming pattern of Pseudomonas aeruginosa near a vertical wall during initial attachment stages of biofilm formation
title_full	Dynamic swimming pattern of Pseudomonas aeruginosa near a vertical wall during initial attachment stages of biofilm formation
title_fullStr	Dynamic swimming pattern of Pseudomonas aeruginosa near a vertical wall during initial attachment stages of biofilm formation
title_full_unstemmed	Dynamic swimming pattern of Pseudomonas aeruginosa near a vertical wall during initial attachment stages of biofilm formation
title_sort	dynamic swimming pattern of pseudomonas aeruginosa near a vertical wall during initial attachment stages of biofilm formation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/60cb3094c858476a99b1f4a0c3b6f43b
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Dynamic swimming pattern of Pseudomonas aeruginosa near a vertical wall during initial attachment stages of biofilm formation

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