Crosstalks Among Cancer Stem Cells and Histopathologic Features in Determining Prognosis in Canine Mammary Gland Carcinomas

The purpose of the present work was the evaluation of the prognostic potential of histopathologic features, cancer stem cells (CSCs), and epthelial-mesenchymal transition (EMT) in relation to lymph node status and lymphovascular invasion (LVI) in canine mammary gland carcinomas (CMGCs). CSCs are pr...

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Autores principales:	Marzban Havva, Chamani Gelareh, Khaki Fariba, Amini Parnian, Pedram Nastaran, Asghari Zeinab, Boroomand Mohammadreza, Moradi Samira, Yahyaei Artimes, Hossein Boroujerdi Mersedeh Sadat, Valinezhad Kamyab
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2021
Materias:	cancer stem cells canine carcinoma epithelial-mesenchymal transition immunohistochemistry animal model Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/60d2410b9d114cddacc1cda9eb9f0068
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spelling	oai:doaj.org-article:60d2410b9d114cddacc1cda9eb9f00682021-11-17T21:27:54ZCrosstalks Among Cancer Stem Cells and Histopathologic Features in Determining Prognosis in Canine Mammary Gland Carcinomas1820-744810.2478/acve-2021-0026https://doaj.org/article/60d2410b9d114cddacc1cda9eb9f00682021-09-01T00:00:00Zhttps://doi.org/10.2478/acve-2021-0026https://doaj.org/toc/1820-7448The purpose of the present work was the evaluation of the prognostic potential of histopathologic features, cancer stem cells (CSCs), and epthelial-mesenchymal transition (EMT) in relation to lymph node status and lymphovascular invasion (LVI) in canine mammary gland carcinomas (CMGCs). CSCs are proposed as the main cause of tumorigenesis, therapy failure, and recurrence which form a small fraction of tumor bulk. We evaluated presence of micropapillary growth pattern (MGP), infiltration into surrounding tissues (IST), and vasculogenic mimicry (VM) in H&E stained slides of 26 paraffin-embedded tumor samples. Lymph nodes of all cases were assessed. Additionally, they were examined immunohistochemically in terms of vimentin expression as an indicator of EMT which is a well-known mechanism for metastasis, and CD44, CD24, and ALDH1 for CSCs detection. Data analyses showed significant relationships between MGP and CSCs (P = 0.037), VM and CSCs (P = 0.013), lymph node status and CSCs (P = 0.0001), lymph node status and EMT (P = 0.003), IST and LVI (P = 0.05), VM and LVI (P = 0.01), VM and lymph node status (P = 0.007), and LVI and lymph node status (P = 0.04). Results indicated the prognostic value of MGP, VM, and CSCs with respect to confirmed prognostic markers, including LVI and lymph node involvement, in CMGCs.Marzban HavvaChamani GelarehKhaki FaribaAmini ParnianPedram NastaranAsghari ZeinabBoroomand MohammadrezaMoradi SamiraYahyaei ArtimesHossein Boroujerdi Mersedeh SadatValinezhad KamyabSciendoarticlecancer stem cellscaninecarcinomaepithelial-mesenchymal transitionimmunohistochemistryanimal modelVeterinary medicineSF600-1100ENActa Veterinaria, Vol 71, Iss 3, Pp 303-317 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cancer stem cells canine carcinoma epithelial-mesenchymal transition immunohistochemistry animal model Veterinary medicine SF600-1100
spellingShingle	cancer stem cells canine carcinoma epithelial-mesenchymal transition immunohistochemistry animal model Veterinary medicine SF600-1100 Marzban Havva Chamani Gelareh Khaki Fariba Amini Parnian Pedram Nastaran Asghari Zeinab Boroomand Mohammadreza Moradi Samira Yahyaei Artimes Hossein Boroujerdi Mersedeh Sadat Valinezhad Kamyab Crosstalks Among Cancer Stem Cells and Histopathologic Features in Determining Prognosis in Canine Mammary Gland Carcinomas
description	The purpose of the present work was the evaluation of the prognostic potential of histopathologic features, cancer stem cells (CSCs), and epthelial-mesenchymal transition (EMT) in relation to lymph node status and lymphovascular invasion (LVI) in canine mammary gland carcinomas (CMGCs). CSCs are proposed as the main cause of tumorigenesis, therapy failure, and recurrence which form a small fraction of tumor bulk. We evaluated presence of micropapillary growth pattern (MGP), infiltration into surrounding tissues (IST), and vasculogenic mimicry (VM) in H&E stained slides of 26 paraffin-embedded tumor samples. Lymph nodes of all cases were assessed. Additionally, they were examined immunohistochemically in terms of vimentin expression as an indicator of EMT which is a well-known mechanism for metastasis, and CD44, CD24, and ALDH1 for CSCs detection. Data analyses showed significant relationships between MGP and CSCs (P = 0.037), VM and CSCs (P = 0.013), lymph node status and CSCs (P = 0.0001), lymph node status and EMT (P = 0.003), IST and LVI (P = 0.05), VM and LVI (P = 0.01), VM and lymph node status (P = 0.007), and LVI and lymph node status (P = 0.04). Results indicated the prognostic value of MGP, VM, and CSCs with respect to confirmed prognostic markers, including LVI and lymph node involvement, in CMGCs.
format	article
author	Marzban Havva Chamani Gelareh Khaki Fariba Amini Parnian Pedram Nastaran Asghari Zeinab Boroomand Mohammadreza Moradi Samira Yahyaei Artimes Hossein Boroujerdi Mersedeh Sadat Valinezhad Kamyab
author_facet	Marzban Havva Chamani Gelareh Khaki Fariba Amini Parnian Pedram Nastaran Asghari Zeinab Boroomand Mohammadreza Moradi Samira Yahyaei Artimes Hossein Boroujerdi Mersedeh Sadat Valinezhad Kamyab
author_sort	Marzban Havva
title	Crosstalks Among Cancer Stem Cells and Histopathologic Features in Determining Prognosis in Canine Mammary Gland Carcinomas
title_short	Crosstalks Among Cancer Stem Cells and Histopathologic Features in Determining Prognosis in Canine Mammary Gland Carcinomas
title_full	Crosstalks Among Cancer Stem Cells and Histopathologic Features in Determining Prognosis in Canine Mammary Gland Carcinomas
title_fullStr	Crosstalks Among Cancer Stem Cells and Histopathologic Features in Determining Prognosis in Canine Mammary Gland Carcinomas
title_full_unstemmed	Crosstalks Among Cancer Stem Cells and Histopathologic Features in Determining Prognosis in Canine Mammary Gland Carcinomas
title_sort	crosstalks among cancer stem cells and histopathologic features in determining prognosis in canine mammary gland carcinomas
publisher	Sciendo
publishDate	2021
url	https://doaj.org/article/60d2410b9d114cddacc1cda9eb9f0068
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Crosstalks Among Cancer Stem Cells and Histopathologic Features in Determining Prognosis in Canine Mammary Gland Carcinomas

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