Zn2+-Dependent Nuclease Is Involved in Nuclear Degradation during the Programmed Cell Death of Secretory Cavity Formation in Citrus grandis ‘Tomentosa’ Fruits

Zn2+- and Ca2+-dependent nucleases exhibit activity toward dsDNA in the four classes of cation-dependent nucleases in plants. Programmed cell death (PCD) is involved in the degradation of cells during schizolysigenous secretory cavity formation in <i&...

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Autores principales:	Minjian Liang, Mei Bai, Hong Wu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	secretory cavity PCD CgENDO1 Zn<sup>2+</sup> ions nuclear DNA degradation Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/60d8c0dcb8e84c0a9193aecb927dd0f9
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spelling	oai:doaj.org-article:60d8c0dcb8e84c0a9193aecb927dd0f92021-11-25T17:13:02ZZn<sup>2+</sup>-Dependent Nuclease Is Involved in Nuclear Degradation during the Programmed Cell Death of Secretory Cavity Formation in <i>Citrus grandis</i> ‘Tomentosa’ Fruits10.3390/cells101132222073-4409https://doaj.org/article/60d8c0dcb8e84c0a9193aecb927dd0f92021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/3222https://doaj.org/toc/2073-4409Zn<sup>2+</sup>- and Ca<sup>2+</sup>-dependent nucleases exhibit activity toward dsDNA in the four classes of cation-dependent nucleases in plants. Programmed cell death (PCD) is involved in the degradation of cells during schizolysigenous secretory cavity formation in <i>Citrus</i> fruits. Recently, the Ca<sup>2+</sup>-dependent DNase CgCAN was proven to play a key role in nuclear DNA degradation during the PCD of secretory cavity formation in <i>Citrus grandis</i> ‘Tomentosa’ fruits. However, whether Zn<sup>2+</sup>-dependent nuclease plays a role in the PCD of secretory cells remains poorly understood. Here, we identified a Zn<sup>2+</sup>-dependent nuclease gene, <i>CgENDO1</i>, from <i>Citrus</i> <i>grandis</i> ‘Tomentosa’, the function of which was studied using Zn<sup>2+</sup> ions cytochemical localization, DNase activity assays, in situ hybridization, and protein immunolocalization. The full-length cDNA of <i>CgENDO1</i> contains an open reading frame of 906 bp that encodes a protein 301 amino acids in length with a S1/P1-like functional domain. CgENDO1 degrades linear double-stranded DNA at acidic and neutral pH. <i>CgENDO1</i> is mainly expressed in the late stage of nuclear degradation of secretory cells. Further spatiotemporal expression patterns of CgENDO1 showed that CgENDO1 is initially located on the endoplasmic reticulum and then moves into intracellular vesicles and nuclei. During the late stage of nuclear degradation, it was concentrated in the area of nuclear degradation involved in nuclear DNA degradation. Our results suggest that the Zn<sup>2+</sup>-dependent nuclease CgENDO1 plays a direct role in the late degradation stage of the nuclear DNA in the PCD of secretory cavity cells of <i>Citrus grandis</i> ‘Tomentosa’ fruits.Minjian LiangMei BaiHong WuMDPI AGarticlesecretory cavityPCDCgENDO1Zn<sup>2+</sup> ionsnuclear DNA degradationBiology (General)QH301-705.5ENCells, Vol 10, Iss 3222, p 3222 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	secretory cavity PCD CgENDO1 Zn<sup>2+</sup> ions nuclear DNA degradation Biology (General) QH301-705.5
spellingShingle	secretory cavity PCD CgENDO1 Zn<sup>2+</sup> ions nuclear DNA degradation Biology (General) QH301-705.5 Minjian Liang Mei Bai Hong Wu Zn<sup>2+</sup>-Dependent Nuclease Is Involved in Nuclear Degradation during the Programmed Cell Death of Secretory Cavity Formation in <i>Citrus grandis</i> ‘Tomentosa’ Fruits
description	Zn<sup>2+</sup>- and Ca<sup>2+</sup>-dependent nucleases exhibit activity toward dsDNA in the four classes of cation-dependent nucleases in plants. Programmed cell death (PCD) is involved in the degradation of cells during schizolysigenous secretory cavity formation in <i>Citrus</i> fruits. Recently, the Ca<sup>2+</sup>-dependent DNase CgCAN was proven to play a key role in nuclear DNA degradation during the PCD of secretory cavity formation in <i>Citrus grandis</i> ‘Tomentosa’ fruits. However, whether Zn<sup>2+</sup>-dependent nuclease plays a role in the PCD of secretory cells remains poorly understood. Here, we identified a Zn<sup>2+</sup>-dependent nuclease gene, <i>CgENDO1</i>, from <i>Citrus</i> <i>grandis</i> ‘Tomentosa’, the function of which was studied using Zn<sup>2+</sup> ions cytochemical localization, DNase activity assays, in situ hybridization, and protein immunolocalization. The full-length cDNA of <i>CgENDO1</i> contains an open reading frame of 906 bp that encodes a protein 301 amino acids in length with a S1/P1-like functional domain. CgENDO1 degrades linear double-stranded DNA at acidic and neutral pH. <i>CgENDO1</i> is mainly expressed in the late stage of nuclear degradation of secretory cells. Further spatiotemporal expression patterns of CgENDO1 showed that CgENDO1 is initially located on the endoplasmic reticulum and then moves into intracellular vesicles and nuclei. During the late stage of nuclear degradation, it was concentrated in the area of nuclear degradation involved in nuclear DNA degradation. Our results suggest that the Zn<sup>2+</sup>-dependent nuclease CgENDO1 plays a direct role in the late degradation stage of the nuclear DNA in the PCD of secretory cavity cells of <i>Citrus grandis</i> ‘Tomentosa’ fruits.
format	article
author	Minjian Liang Mei Bai Hong Wu
author_facet	Minjian Liang Mei Bai Hong Wu
author_sort	Minjian Liang
title	Zn<sup>2+</sup>-Dependent Nuclease Is Involved in Nuclear Degradation during the Programmed Cell Death of Secretory Cavity Formation in <i>Citrus grandis</i> ‘Tomentosa’ Fruits
title_short	Zn<sup>2+</sup>-Dependent Nuclease Is Involved in Nuclear Degradation during the Programmed Cell Death of Secretory Cavity Formation in <i>Citrus grandis</i> ‘Tomentosa’ Fruits
title_full	Zn<sup>2+</sup>-Dependent Nuclease Is Involved in Nuclear Degradation during the Programmed Cell Death of Secretory Cavity Formation in <i>Citrus grandis</i> ‘Tomentosa’ Fruits
title_fullStr	Zn<sup>2+</sup>-Dependent Nuclease Is Involved in Nuclear Degradation during the Programmed Cell Death of Secretory Cavity Formation in <i>Citrus grandis</i> ‘Tomentosa’ Fruits
title_full_unstemmed	Zn<sup>2+</sup>-Dependent Nuclease Is Involved in Nuclear Degradation during the Programmed Cell Death of Secretory Cavity Formation in <i>Citrus grandis</i> ‘Tomentosa’ Fruits
title_sort	zn<sup>2+</sup>-dependent nuclease is involved in nuclear degradation during the programmed cell death of secretory cavity formation in <i>citrus grandis</i> ‘tomentosa’ fruits
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/60d8c0dcb8e84c0a9193aecb927dd0f9
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Zn<sup>2+</sup>-Dependent Nuclease Is Involved in Nuclear Degradation during the Programmed Cell Death of Secretory Cavity Formation in <i>Citrus grandis</i> ‘Tomentosa’ Fruits

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