A rationally enhanced red fluorescent protein expands the utility of FRET biosensors
TagRFP is a bright red fluorescent protein, but undergoes photoconversion to a dark state, making it undesirable for conventional fluorescence microscopy. Here, the authors introduce stabilising mutations to create super-TagRFP (stagRFP) and demonstrate its application as both a FRET acceptor and FR...
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| Auteurs principaux: | , , , , |
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| Format: | article |
| Langue: | EN |
| Publié: |
Nature Portfolio
2020
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| Sujets: | |
| Accès en ligne: | https://doaj.org/article/60e52827acdd49bbad631b9bd047a4e2 |
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| Résumé: | TagRFP is a bright red fluorescent protein, but undergoes photoconversion to a dark state, making it undesirable for conventional fluorescence microscopy. Here, the authors introduce stabilising mutations to create super-TagRFP (stagRFP) and demonstrate its application as both a FRET acceptor and FRET donor. |
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