A rationally enhanced red fluorescent protein expands the utility of FRET biosensors

TagRFP is a bright red fluorescent protein, but undergoes photoconversion to a dark state, making it undesirable for conventional fluorescence microscopy. Here, the authors introduce stabilising mutations to create super-TagRFP (stagRFP) and demonstrate its application as both a FRET acceptor and FR...

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Autores principales:	Gary C. H. Mo, Clara Posner, Erik A. Rodriguez, Tengqian Sun, Jin Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/60e52827acdd49bbad631b9bd047a4e2
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id	oai:doaj.org-article:60e52827acdd49bbad631b9bd047a4e2
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spelling	oai:doaj.org-article:60e52827acdd49bbad631b9bd047a4e22021-12-02T14:42:47ZA rationally enhanced red fluorescent protein expands the utility of FRET biosensors10.1038/s41467-020-15687-x2041-1723https://doaj.org/article/60e52827acdd49bbad631b9bd047a4e22020-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-15687-xhttps://doaj.org/toc/2041-1723TagRFP is a bright red fluorescent protein, but undergoes photoconversion to a dark state, making it undesirable for conventional fluorescence microscopy. Here, the authors introduce stabilising mutations to create super-TagRFP (stagRFP) and demonstrate its application as both a FRET acceptor and FRET donor.Gary C. H. MoClara PosnerErik A. RodriguezTengqian SunJin ZhangNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-9 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Gary C. H. Mo Clara Posner Erik A. Rodriguez Tengqian Sun Jin Zhang A rationally enhanced red fluorescent protein expands the utility of FRET biosensors
description	TagRFP is a bright red fluorescent protein, but undergoes photoconversion to a dark state, making it undesirable for conventional fluorescence microscopy. Here, the authors introduce stabilising mutations to create super-TagRFP (stagRFP) and demonstrate its application as both a FRET acceptor and FRET donor.
format	article
author	Gary C. H. Mo Clara Posner Erik A. Rodriguez Tengqian Sun Jin Zhang
author_facet	Gary C. H. Mo Clara Posner Erik A. Rodriguez Tengqian Sun Jin Zhang
author_sort	Gary C. H. Mo
title	A rationally enhanced red fluorescent protein expands the utility of FRET biosensors
title_short	A rationally enhanced red fluorescent protein expands the utility of FRET biosensors
title_full	A rationally enhanced red fluorescent protein expands the utility of FRET biosensors
title_fullStr	A rationally enhanced red fluorescent protein expands the utility of FRET biosensors
title_full_unstemmed	A rationally enhanced red fluorescent protein expands the utility of FRET biosensors
title_sort	rationally enhanced red fluorescent protein expands the utility of fret biosensors
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/60e52827acdd49bbad631b9bd047a4e2
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A rationally enhanced red fluorescent protein expands the utility of FRET biosensors

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