Screening of Swiss Pig Herds for Hepatitis E Virus: A Pilot Study

Hepatitis E virus (HEV) is an important cause of acute hepatitis in humans worldwide. In industrialised countries, most infections are caused by the zoonotic genotype 3. The main reservoir was found in pigs, with fattening pigs as the main shedders. The aim of this study was to establish a screening...

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Autores principales:	Julia Lienhard, Isabelle Vonlanthen-Specker, Xaver Sidler, Claudia Bachofen
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	hepatitis E virus pig screening genotyping Switzerland Veterinary medicine SF600-1100 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/60e6aab02aa84e57a71544465b34130e
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spelling	oai:doaj.org-article:60e6aab02aa84e57a71544465b34130e2021-11-25T16:14:10ZScreening of Swiss Pig Herds for Hepatitis E Virus: A Pilot Study10.3390/ani111130502076-2615https://doaj.org/article/60e6aab02aa84e57a71544465b34130e2021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2615/11/11/3050https://doaj.org/toc/2076-2615Hepatitis E virus (HEV) is an important cause of acute hepatitis in humans worldwide. In industrialised countries, most infections are caused by the zoonotic genotype 3. The main reservoir was found in pigs, with fattening pigs as the main shedders. The aim of this study was to establish a screening tool to detect HEV in pig farms. HEV-positive samples were sequenced using Sanger sequencing. First, different sample materials, including floor swabs, slurry, dust swabs and faeces were tested for HEV. Floor swabs turned out to give the best results and, in the form of sock swabs, were used for the screening of Swiss pig herds. A total of 138 pig farms were tested, with a focus on fattening pigs. Overall, 81 farms (58.8%) were HEV positive. Most sequences belonged to subtype 3h, in which they formed a specific cluster (Swiss cluster). In addition, subtype 3l and two unassigned sequences were detected. As a conclusion, sock swabs were found to be a helpful tool to screen pig herds for HEV and establish a sequence collection that may enable molecular epidemiology and support outbreak investigation and prevention.Julia LienhardIsabelle Vonlanthen-SpeckerXaver SidlerClaudia BachofenMDPI AGarticlehepatitis E viruspigscreeninggenotypingSwitzerlandVeterinary medicineSF600-1100ZoologyQL1-991ENAnimals, Vol 11, Iss 3050, p 3050 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	hepatitis E virus pig screening genotyping Switzerland Veterinary medicine SF600-1100 Zoology QL1-991
spellingShingle	hepatitis E virus pig screening genotyping Switzerland Veterinary medicine SF600-1100 Zoology QL1-991 Julia Lienhard Isabelle Vonlanthen-Specker Xaver Sidler Claudia Bachofen Screening of Swiss Pig Herds for Hepatitis E Virus: A Pilot Study
description	Hepatitis E virus (HEV) is an important cause of acute hepatitis in humans worldwide. In industrialised countries, most infections are caused by the zoonotic genotype 3. The main reservoir was found in pigs, with fattening pigs as the main shedders. The aim of this study was to establish a screening tool to detect HEV in pig farms. HEV-positive samples were sequenced using Sanger sequencing. First, different sample materials, including floor swabs, slurry, dust swabs and faeces were tested for HEV. Floor swabs turned out to give the best results and, in the form of sock swabs, were used for the screening of Swiss pig herds. A total of 138 pig farms were tested, with a focus on fattening pigs. Overall, 81 farms (58.8%) were HEV positive. Most sequences belonged to subtype 3h, in which they formed a specific cluster (Swiss cluster). In addition, subtype 3l and two unassigned sequences were detected. As a conclusion, sock swabs were found to be a helpful tool to screen pig herds for HEV and establish a sequence collection that may enable molecular epidemiology and support outbreak investigation and prevention.
format	article
author	Julia Lienhard Isabelle Vonlanthen-Specker Xaver Sidler Claudia Bachofen
author_facet	Julia Lienhard Isabelle Vonlanthen-Specker Xaver Sidler Claudia Bachofen
author_sort	Julia Lienhard
title	Screening of Swiss Pig Herds for Hepatitis E Virus: A Pilot Study
title_short	Screening of Swiss Pig Herds for Hepatitis E Virus: A Pilot Study
title_full	Screening of Swiss Pig Herds for Hepatitis E Virus: A Pilot Study
title_fullStr	Screening of Swiss Pig Herds for Hepatitis E Virus: A Pilot Study
title_full_unstemmed	Screening of Swiss Pig Herds for Hepatitis E Virus: A Pilot Study
title_sort	screening of swiss pig herds for hepatitis e virus: a pilot study
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/60e6aab02aa84e57a71544465b34130e
work_keys_str_mv	AT julialienhard screeningofswisspigherdsforhepatitisevirusapilotstudy AT isabellevonlanthenspecker screeningofswisspigherdsforhepatitisevirusapilotstudy AT xaversidler screeningofswisspigherdsforhepatitisevirusapilotstudy AT claudiabachofen screeningofswisspigherdsforhepatitisevirusapilotstudy
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Screening of Swiss Pig Herds for Hepatitis E Virus: A Pilot Study

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