Spermine-modified Antheraea pernyi silk fibroin as a gene delivery carrier

Yanni Yu,1 Yongpei Hu,1 Xiufang Li,1 Yu Liu,1 Mingzhong Li,1 Jicheng Yang,2 Weihua Sheng2 1National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, 2Cell and Molecular Biology Institute, College of Medicine, Soochow University, Suzhou, People...

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Autores principales:	Yu Y, Hu Y, Li X, Liu Y, Li M, Yang J, Sheng W
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	silk fibroin spermine cationized polymer gene delivery vector Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/60e8acc4343e44d0ace3fa52dd99248c
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spelling	oai:doaj.org-article:60e8acc4343e44d0ace3fa52dd99248c2021-12-02T01:49:54ZSpermine-modified Antheraea pernyi silk fibroin as a gene delivery carrier1178-2013https://doaj.org/article/60e8acc4343e44d0ace3fa52dd99248c2016-03-01T00:00:00Zhttps://www.dovepress.com/spermine-modified-antheraea-pernyi-silk-fibroin-as-a-gene-delivery-car-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yanni Yu,1 Yongpei Hu,1 Xiufang Li,1 Yu Liu,1 Mingzhong Li,1 Jicheng Yang,2 Weihua Sheng2 1National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, 2Cell and Molecular Biology Institute, College of Medicine, Soochow University, Suzhou, People’s Republic of China Abstract: The development of a novel cationized polymer used as a gene delivery carrier that can conveniently and effectively transfect cells resulting in a stably expressed target gene remains a challenge. Antheraea pernyi silk fibroin (ASF) is a cytocompatible and biodegradable natural polymer, and it possesses Arg–Gly–Asp sequences but a negative charge. In order to render ASF amenable to packaging plasmid DNA (pDNA), spermine was used to modify ASF to synthesize cationized ASF (CASF), which was used as a gene delivery carrier. CASF was characterized using trinitrobenzene sulfonic acid assay, the zeta potential determination, and a Fourier transform infrared analysis, and the results of these characterizations indicated that the –NH2 in spermine effectively reacts with the –COOH in the side chains of ASF. Spermine grafted to the side chains of ASF resulted in the conversion of the negative charge of ASF to a positive charge. CASF packaged pDNA and formed CASF/pDNA complexes, which exhibited spherical morphology with average particle sizes of 215–281 nm and zeta potential of approximately +3.0 mV to +3.2 mV. The results of the MTT assay, confocal laser scanning microscopy, and flow cytometry analysis in a human endothelial cell line revealed that CASF/pDNA complexes exhibited lower cytotoxicity and higher transfection efficiency compared to the pDNA complexes of polyethyleneimine. These results indicate that our synthesized CASF, a cationized polymer, is a potential gene delivery carrier with the advantages of biodegradability and low cytotoxicity. Keywords: silk fibroin, spermine, cationized polymer, gene delivery carrierYu YHu YLi XLiu YLi MYang JSheng WDove Medical Pressarticlesilk fibroinsperminecationized polymergene delivery vectorMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss default, Pp 1013-1023 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	silk fibroin spermine cationized polymer gene delivery vector Medicine (General) R5-920
spellingShingle	silk fibroin spermine cationized polymer gene delivery vector Medicine (General) R5-920 Yu Y Hu Y Li X Liu Y Li M Yang J Sheng W Spermine-modified Antheraea pernyi silk fibroin as a gene delivery carrier
description	Yanni Yu,1 Yongpei Hu,1 Xiufang Li,1 Yu Liu,1 Mingzhong Li,1 Jicheng Yang,2 Weihua Sheng2 1National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, 2Cell and Molecular Biology Institute, College of Medicine, Soochow University, Suzhou, People’s Republic of China Abstract: The development of a novel cationized polymer used as a gene delivery carrier that can conveniently and effectively transfect cells resulting in a stably expressed target gene remains a challenge. Antheraea pernyi silk fibroin (ASF) is a cytocompatible and biodegradable natural polymer, and it possesses Arg–Gly–Asp sequences but a negative charge. In order to render ASF amenable to packaging plasmid DNA (pDNA), spermine was used to modify ASF to synthesize cationized ASF (CASF), which was used as a gene delivery carrier. CASF was characterized using trinitrobenzene sulfonic acid assay, the zeta potential determination, and a Fourier transform infrared analysis, and the results of these characterizations indicated that the –NH2 in spermine effectively reacts with the –COOH in the side chains of ASF. Spermine grafted to the side chains of ASF resulted in the conversion of the negative charge of ASF to a positive charge. CASF packaged pDNA and formed CASF/pDNA complexes, which exhibited spherical morphology with average particle sizes of 215–281 nm and zeta potential of approximately +3.0 mV to +3.2 mV. The results of the MTT assay, confocal laser scanning microscopy, and flow cytometry analysis in a human endothelial cell line revealed that CASF/pDNA complexes exhibited lower cytotoxicity and higher transfection efficiency compared to the pDNA complexes of polyethyleneimine. These results indicate that our synthesized CASF, a cationized polymer, is a potential gene delivery carrier with the advantages of biodegradability and low cytotoxicity. Keywords: silk fibroin, spermine, cationized polymer, gene delivery carrier
format	article
author	Yu Y Hu Y Li X Liu Y Li M Yang J Sheng W
author_facet	Yu Y Hu Y Li X Liu Y Li M Yang J Sheng W
author_sort	Yu Y
title	Spermine-modified Antheraea pernyi silk fibroin as a gene delivery carrier
title_short	Spermine-modified Antheraea pernyi silk fibroin as a gene delivery carrier
title_full	Spermine-modified Antheraea pernyi silk fibroin as a gene delivery carrier
title_fullStr	Spermine-modified Antheraea pernyi silk fibroin as a gene delivery carrier
title_full_unstemmed	Spermine-modified Antheraea pernyi silk fibroin as a gene delivery carrier
title_sort	spermine-modified antheraea pernyi silk fibroin as a gene delivery carrier
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/60e8acc4343e44d0ace3fa52dd99248c
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Spermine-modified Antheraea pernyi silk fibroin as a gene delivery carrier

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