Strongly Adhesive and Antimicrobial Peptide-Loaded, Alginate–Catechol-Based Gels for Application against Periimplantitis

Background: Periimplantitis is a disease linked to oral virulent bacteria such as <i>P. gingivalis</i> that grow in dental implants surrounding tissues and between implants and abutments. Antimicrobial gels previously described to fill these sites lose their effectiveness and resorb over...

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Autores principales:	Sébastien Baixe, Vincent Ball, Loïc Jierry, Sarah Cianférani, Jean-Marc Strub, Youssef Haikel, Marie-Hélène Metz-Boutigue, Olivier Etienne
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	hydrogel AMP Alginate–Catechol periimplantitis <i>P. gingivalis</i> rheology Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/60ec1ff7eabe4d2cb1976ee31b1befb1
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spelling	oai:doaj.org-article:60ec1ff7eabe4d2cb1976ee31b1befb12021-11-11T15:07:39ZStrongly Adhesive and Antimicrobial Peptide-Loaded, Alginate–Catechol-Based Gels for Application against Periimplantitis10.3390/app1121100502076-3417https://doaj.org/article/60ec1ff7eabe4d2cb1976ee31b1befb12021-10-01T00:00:00Zhttps://www.mdpi.com/2076-3417/11/21/10050https://doaj.org/toc/2076-3417Background: Periimplantitis is a disease linked to oral virulent bacteria such as <i>P. gingivalis</i> that grow in dental implants surrounding tissues and between implants and abutments. Antimicrobial gels previously described to fill these sites lose their effectiveness and resorb over time. Objective: Characterization of biophysical and antimicrobial properties of an original hydrogel, Alginate–Catechol (Alg–Cat), combined to D-Cateslytin (D-CTL). Methods: Gelation kinetics, frequency and strain sweep measurements were performed by rheology. Antibacterial activity of the gels was tested against <i>P. gingivalis</i>, and the MIC was determined. Peptides released from the gels were purified by HPLC and characterized by MALDI–TOF mass spectrometry. The behavior of bacteria in contact with the gel was observed using optical and electronic microscopy (SEM and TEM). Results: Gelation was fast and was achieved in 2 min with a storage modulus between 25 and 30 Pa. The gels were stable under strain and showed an adhesive potential reinforced with aging at 18 h (5.4 kPa) under a slow retraction speed (4 J·m<sup>−2</sup> at 10 µm/s) with a mixed rupture profile (adhesive/cohesive). The MIC of D-CTL inside the Alg–Cat gel against <i>P. gingivalis</i> was equal to 470 µg·mL<sup>−1</sup> after 24 h. Peptides recovered in the supernatant and inside the gel were fragmented, most of them conserving the ⍺-helix active site. No bacteria were visualized at the surface and inside the gel after 24 h. This gel is promising for clinical application for the prevention of periimplantitis.Sébastien BaixeVincent BallLoïc JierrySarah CianféraniJean-Marc StrubYoussef HaikelMarie-Hélène Metz-BoutigueOlivier EtienneMDPI AGarticlehydrogelAMPAlginate–Catecholperiimplantitis<i>P. gingivalis</i>rheologyTechnologyTEngineering (General). Civil engineering (General)TA1-2040Biology (General)QH301-705.5PhysicsQC1-999ChemistryQD1-999ENApplied Sciences, Vol 11, Iss 10050, p 10050 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	hydrogel AMP Alginate–Catechol periimplantitis <i>P. gingivalis</i> rheology Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999
spellingShingle	hydrogel AMP Alginate–Catechol periimplantitis <i>P. gingivalis</i> rheology Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999 Sébastien Baixe Vincent Ball Loïc Jierry Sarah Cianférani Jean-Marc Strub Youssef Haikel Marie-Hélène Metz-Boutigue Olivier Etienne Strongly Adhesive and Antimicrobial Peptide-Loaded, Alginate–Catechol-Based Gels for Application against Periimplantitis
description	Background: Periimplantitis is a disease linked to oral virulent bacteria such as <i>P. gingivalis</i> that grow in dental implants surrounding tissues and between implants and abutments. Antimicrobial gels previously described to fill these sites lose their effectiveness and resorb over time. Objective: Characterization of biophysical and antimicrobial properties of an original hydrogel, Alginate–Catechol (Alg–Cat), combined to D-Cateslytin (D-CTL). Methods: Gelation kinetics, frequency and strain sweep measurements were performed by rheology. Antibacterial activity of the gels was tested against <i>P. gingivalis</i>, and the MIC was determined. Peptides released from the gels were purified by HPLC and characterized by MALDI–TOF mass spectrometry. The behavior of bacteria in contact with the gel was observed using optical and electronic microscopy (SEM and TEM). Results: Gelation was fast and was achieved in 2 min with a storage modulus between 25 and 30 Pa. The gels were stable under strain and showed an adhesive potential reinforced with aging at 18 h (5.4 kPa) under a slow retraction speed (4 J·m<sup>−2</sup> at 10 µm/s) with a mixed rupture profile (adhesive/cohesive). The MIC of D-CTL inside the Alg–Cat gel against <i>P. gingivalis</i> was equal to 470 µg·mL<sup>−1</sup> after 24 h. Peptides recovered in the supernatant and inside the gel were fragmented, most of them conserving the ⍺-helix active site. No bacteria were visualized at the surface and inside the gel after 24 h. This gel is promising for clinical application for the prevention of periimplantitis.
format	article
author	Sébastien Baixe Vincent Ball Loïc Jierry Sarah Cianférani Jean-Marc Strub Youssef Haikel Marie-Hélène Metz-Boutigue Olivier Etienne
author_facet	Sébastien Baixe Vincent Ball Loïc Jierry Sarah Cianférani Jean-Marc Strub Youssef Haikel Marie-Hélène Metz-Boutigue Olivier Etienne
author_sort	Sébastien Baixe
title	Strongly Adhesive and Antimicrobial Peptide-Loaded, Alginate–Catechol-Based Gels for Application against Periimplantitis
title_short	Strongly Adhesive and Antimicrobial Peptide-Loaded, Alginate–Catechol-Based Gels for Application against Periimplantitis
title_full	Strongly Adhesive and Antimicrobial Peptide-Loaded, Alginate–Catechol-Based Gels for Application against Periimplantitis
title_fullStr	Strongly Adhesive and Antimicrobial Peptide-Loaded, Alginate–Catechol-Based Gels for Application against Periimplantitis
title_full_unstemmed	Strongly Adhesive and Antimicrobial Peptide-Loaded, Alginate–Catechol-Based Gels for Application against Periimplantitis
title_sort	strongly adhesive and antimicrobial peptide-loaded, alginate–catechol-based gels for application against periimplantitis
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/60ec1ff7eabe4d2cb1976ee31b1befb1
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Strongly Adhesive and Antimicrobial Peptide-Loaded, Alginate–Catechol-Based Gels for Application against Periimplantitis

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