Electronic control of redox reactions inside Escherichia coli using a genetic module

Microorganisms regulate the redox state of different biomolecules to precisely control biological processes. These processes can be modulated by electrochemically coupling intracellular biomolecules to an external electrode, but current approaches afford only limited control and specificity. Here we...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Moshe Baruch, Sara Tejedor-Sanz, Lin Su, Caroline M. Ajo-Franklin
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/60fd0e68cbad48139d86fae059fec62b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:60fd0e68cbad48139d86fae059fec62b
record_format	dspace
spelling	oai:doaj.org-article:60fd0e68cbad48139d86fae059fec62b2021-11-25T06:19:38ZElectronic control of redox reactions inside Escherichia coli using a genetic module1932-6203https://doaj.org/article/60fd0e68cbad48139d86fae059fec62b2021-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8601525/?tool=EBIhttps://doaj.org/toc/1932-6203Microorganisms regulate the redox state of different biomolecules to precisely control biological processes. These processes can be modulated by electrochemically coupling intracellular biomolecules to an external electrode, but current approaches afford only limited control and specificity. Here we describe specific electrochemical control of the reduction of intracellular biomolecules in Escherichia coli through introduction of a heterologous electron transfer pathway. E. coli expressing cymAmtrCAB from Shewanella oneidensis MR-1 consumed electrons directly from a cathode when fumarate or nitrate, both intracellular electron acceptors, were present. The fumarate-triggered current consumption occurred only when fumarate reductase was present, indicating all the electrons passed through this enzyme. Moreover, CymAMtrCAB-expressing E. coli used current to stoichiometrically reduce nitrate. Thus, our work introduces a modular genetic tool to reduce a specific intracellular redox molecule with an electrode, opening the possibility of electronically controlling biological processes such as biosynthesis and growth in any microorganism.Moshe BaruchSara Tejedor-SanzLin SuCaroline M. Ajo-FranklinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Moshe Baruch Sara Tejedor-Sanz Lin Su Caroline M. Ajo-Franklin Electronic control of redox reactions inside Escherichia coli using a genetic module
description	Microorganisms regulate the redox state of different biomolecules to precisely control biological processes. These processes can be modulated by electrochemically coupling intracellular biomolecules to an external electrode, but current approaches afford only limited control and specificity. Here we describe specific electrochemical control of the reduction of intracellular biomolecules in Escherichia coli through introduction of a heterologous electron transfer pathway. E. coli expressing cymAmtrCAB from Shewanella oneidensis MR-1 consumed electrons directly from a cathode when fumarate or nitrate, both intracellular electron acceptors, were present. The fumarate-triggered current consumption occurred only when fumarate reductase was present, indicating all the electrons passed through this enzyme. Moreover, CymAMtrCAB-expressing E. coli used current to stoichiometrically reduce nitrate. Thus, our work introduces a modular genetic tool to reduce a specific intracellular redox molecule with an electrode, opening the possibility of electronically controlling biological processes such as biosynthesis and growth in any microorganism.
format	article
author	Moshe Baruch Sara Tejedor-Sanz Lin Su Caroline M. Ajo-Franklin
author_facet	Moshe Baruch Sara Tejedor-Sanz Lin Su Caroline M. Ajo-Franklin
author_sort	Moshe Baruch
title	Electronic control of redox reactions inside Escherichia coli using a genetic module
title_short	Electronic control of redox reactions inside Escherichia coli using a genetic module
title_full	Electronic control of redox reactions inside Escherichia coli using a genetic module
title_fullStr	Electronic control of redox reactions inside Escherichia coli using a genetic module
title_full_unstemmed	Electronic control of redox reactions inside Escherichia coli using a genetic module
title_sort	electronic control of redox reactions inside escherichia coli using a genetic module
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/60fd0e68cbad48139d86fae059fec62b
work_keys_str_mv	AT moshebaruch electroniccontrolofredoxreactionsinsideescherichiacoliusingageneticmodule AT saratejedorsanz electroniccontrolofredoxreactionsinsideescherichiacoliusingageneticmodule AT linsu electroniccontrolofredoxreactionsinsideescherichiacoliusingageneticmodule AT carolinemajofranklin electroniccontrolofredoxreactionsinsideescherichiacoliusingageneticmodule
_version_	1718413865750888448

Electronic control of redox reactions inside Escherichia coli using a genetic module

Ejemplares similares