Peanut-Shaped Gold Nanoparticles with Shells of Ceragenin CSA-131 Display the Ability to Inhibit Ovarian Cancer Growth In Vitro and in a Tumor Xenograft Model

Gold nanoparticles-assisted delivery of antineoplastics into cancerous cells is presented as an effective approach for overcoming the limitations of systemic chemotherapy. Although ceragenins show great potential as anti-cancer agents, in some tumors, effective inhibition of cancer cells proliferati...

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Autores principales:	Ewelina Piktel, Ilona Oscilowska, Łukasz Suprewicz, Joanna Depciuch, Natalia Marcińczyk, Ewa Chabielska, Przemysław Wolak, Katarzyna Głuszek, Justyna Klimek, Piotr M. Zieliński, Michał T. Marzec, Paul B. Savage, Magdalena Parlińska-Wojtan, Robert Bucki
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	nanotechnology drug delivery non-spherical gold nanoparticles gold nanopeanuts ovarian cancer ceragenins Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/6105b3237bbc465ebff3af4e9cf656b3
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spelling	oai:doaj.org-article:6105b3237bbc465ebff3af4e9cf656b32021-11-11T15:31:07ZPeanut-Shaped Gold Nanoparticles with Shells of Ceragenin CSA-131 Display the Ability to Inhibit Ovarian Cancer Growth In Vitro and in a Tumor Xenograft Model10.3390/cancers132154242072-6694https://doaj.org/article/6105b3237bbc465ebff3af4e9cf656b32021-10-01T00:00:00Zhttps://www.mdpi.com/2072-6694/13/21/5424https://doaj.org/toc/2072-6694Gold nanoparticles-assisted delivery of antineoplastics into cancerous cells is presented as an effective approach for overcoming the limitations of systemic chemotherapy. Although ceragenins show great potential as anti-cancer agents, in some tumors, effective inhibition of cancer cells proliferation requires application of ceragenins at doses within their hemolytic range. For the purpose of toxicity/efficiency ratio control, peanut-shaped gold nanoparticles (AuP NPs) were functionalized with a shell of ceragenin CSA-131 and the cytotoxicity of AuP@CSA-131 against ovarian cancer SKOV-3 cells and were then analyzed. In vivo efficiency of intravenously and intratumorally administered CSA-131 and AuP@CSA-131 was examined using a xenograft ovarian cancer model. Serum parameters were estimated using ELISA methods. Comparative analysis revealed that AuP@CSA-131 exerted stronger anti-cancer effects than free ceragenin, which was determined by enhanced ability to induce caspase-dependent apoptosis and autophagy processes via reactive oxygen species (ROS)-mediated pathways. In an animal study, AuP@CSA-131 was characterized by delayed clearance and prolonged blood circulation when compared with free ceragenin, as well as enhanced anti-tumor efficiency, particularly when applied intratumorally. Administration of CSA-131 and AuP@CSA-131 prevented the inflammatory response associated with cancer development. These results present the possibility of employing non-spherical gold nanoparticles as an effective nanoplatform for the delivery of antineoplastics for the treatment of ovarian malignancy.Ewelina PiktelIlona OscilowskaŁukasz SuprewiczJoanna DepciuchNatalia MarcińczykEwa ChabielskaPrzemysław WolakKatarzyna GłuszekJustyna KlimekPiotr M. ZielińskiMichał T. MarzecPaul B. SavageMagdalena Parlińska-WojtanRobert BuckiMDPI AGarticlenanotechnologydrug deliverynon-spherical gold nanoparticlesgold nanopeanutsovarian cancercerageninsNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENCancers, Vol 13, Iss 5424, p 5424 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	nanotechnology drug delivery non-spherical gold nanoparticles gold nanopeanuts ovarian cancer ceragenins Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	nanotechnology drug delivery non-spherical gold nanoparticles gold nanopeanuts ovarian cancer ceragenins Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Ewelina Piktel Ilona Oscilowska Łukasz Suprewicz Joanna Depciuch Natalia Marcińczyk Ewa Chabielska Przemysław Wolak Katarzyna Głuszek Justyna Klimek Piotr M. Zieliński Michał T. Marzec Paul B. Savage Magdalena Parlińska-Wojtan Robert Bucki Peanut-Shaped Gold Nanoparticles with Shells of Ceragenin CSA-131 Display the Ability to Inhibit Ovarian Cancer Growth In Vitro and in a Tumor Xenograft Model
description	Gold nanoparticles-assisted delivery of antineoplastics into cancerous cells is presented as an effective approach for overcoming the limitations of systemic chemotherapy. Although ceragenins show great potential as anti-cancer agents, in some tumors, effective inhibition of cancer cells proliferation requires application of ceragenins at doses within their hemolytic range. For the purpose of toxicity/efficiency ratio control, peanut-shaped gold nanoparticles (AuP NPs) were functionalized with a shell of ceragenin CSA-131 and the cytotoxicity of AuP@CSA-131 against ovarian cancer SKOV-3 cells and were then analyzed. In vivo efficiency of intravenously and intratumorally administered CSA-131 and AuP@CSA-131 was examined using a xenograft ovarian cancer model. Serum parameters were estimated using ELISA methods. Comparative analysis revealed that AuP@CSA-131 exerted stronger anti-cancer effects than free ceragenin, which was determined by enhanced ability to induce caspase-dependent apoptosis and autophagy processes via reactive oxygen species (ROS)-mediated pathways. In an animal study, AuP@CSA-131 was characterized by delayed clearance and prolonged blood circulation when compared with free ceragenin, as well as enhanced anti-tumor efficiency, particularly when applied intratumorally. Administration of CSA-131 and AuP@CSA-131 prevented the inflammatory response associated with cancer development. These results present the possibility of employing non-spherical gold nanoparticles as an effective nanoplatform for the delivery of antineoplastics for the treatment of ovarian malignancy.
format	article
author	Ewelina Piktel Ilona Oscilowska Łukasz Suprewicz Joanna Depciuch Natalia Marcińczyk Ewa Chabielska Przemysław Wolak Katarzyna Głuszek Justyna Klimek Piotr M. Zieliński Michał T. Marzec Paul B. Savage Magdalena Parlińska-Wojtan Robert Bucki
author_facet	Ewelina Piktel Ilona Oscilowska Łukasz Suprewicz Joanna Depciuch Natalia Marcińczyk Ewa Chabielska Przemysław Wolak Katarzyna Głuszek Justyna Klimek Piotr M. Zieliński Michał T. Marzec Paul B. Savage Magdalena Parlińska-Wojtan Robert Bucki
author_sort	Ewelina Piktel
title	Peanut-Shaped Gold Nanoparticles with Shells of Ceragenin CSA-131 Display the Ability to Inhibit Ovarian Cancer Growth In Vitro and in a Tumor Xenograft Model
title_short	Peanut-Shaped Gold Nanoparticles with Shells of Ceragenin CSA-131 Display the Ability to Inhibit Ovarian Cancer Growth In Vitro and in a Tumor Xenograft Model
title_full	Peanut-Shaped Gold Nanoparticles with Shells of Ceragenin CSA-131 Display the Ability to Inhibit Ovarian Cancer Growth In Vitro and in a Tumor Xenograft Model
title_fullStr	Peanut-Shaped Gold Nanoparticles with Shells of Ceragenin CSA-131 Display the Ability to Inhibit Ovarian Cancer Growth In Vitro and in a Tumor Xenograft Model
title_full_unstemmed	Peanut-Shaped Gold Nanoparticles with Shells of Ceragenin CSA-131 Display the Ability to Inhibit Ovarian Cancer Growth In Vitro and in a Tumor Xenograft Model
title_sort	peanut-shaped gold nanoparticles with shells of ceragenin csa-131 display the ability to inhibit ovarian cancer growth in vitro and in a tumor xenograft model
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/6105b3237bbc465ebff3af4e9cf656b3
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Peanut-Shaped Gold Nanoparticles with Shells of Ceragenin CSA-131 Display the Ability to Inhibit Ovarian Cancer Growth In Vitro and in a Tumor Xenograft Model

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