Drosophila Hox genes induce melanized pseudo-tumors when misexpressed in hemocytes

Abstract Hox genes are early determinants of cell identity along the anterior–posterior body axis across bilaterians. Several late non-homeotic functions of Hox genes have emerged in a variety of processes involved in organogenesis in several organisms, including mammals. Several studies have report...

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Autores principales: Titus Ponrathnam, Ravina Saini, Sofia Banu, Rakesh K. Mishra
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Publicado: Nature Portfolio 2021
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spelling oai:doaj.org-article:6117052a54ca414a9ef104e33571a0ee2021-12-02T13:50:49ZDrosophila Hox genes induce melanized pseudo-tumors when misexpressed in hemocytes10.1038/s41598-021-81472-52045-2322https://doaj.org/article/6117052a54ca414a9ef104e33571a0ee2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81472-5https://doaj.org/toc/2045-2322Abstract Hox genes are early determinants of cell identity along the anterior–posterior body axis across bilaterians. Several late non-homeotic functions of Hox genes have emerged in a variety of processes involved in organogenesis in several organisms, including mammals. Several studies have reported the misexpression of Hox genes in a variety of malignancies including acute myeloid leukemia. The Hox genes Dfd, Ubx, abd-A and Abd-B were overexpressed via the UAS-Gal4 system using Cg-Gal4, Lsp2-Gal4, He-Gal4 and HmlD3-Gal4 as specific drivers. Genetic interaction was tested by bringing overexpression lines in heterozygous mutant backgrounds of Polycomb and trithorax group factors. Larvae were visually scored for melanized bodies. Circulating hemocytes were quantified and tested for differentiation. Pupal lethality was assessed. Expression of Dfd, Ubx and abd-A, but not Abd-B in the hematopoietic compartment of Drosophila led to the appearance of circulating melanized bodies, an increase in cell number, cell-autonomous proliferation, and differentiation of hemocytes. Pupal lethality and melanized pseudo-tumors were suppressed in Psc 1 and esc 2 backgrounds while polycomb group member mutations Pc 1 and Su(z)12 3 and trithorax group member mutation TrlR 85 enhanced the phenotype. Dfd, Ubx and abd-A are leukemogenic. Mutations in Polycomb and trithorax group members modulate the leukemogenic phenotype. Our RNAseq of Cg-Gal4 > UAS-abd-A hemocytes may contain genes important to Hox gene induced leukemias.Titus PonrathnamRavina SainiSofia BanuRakesh K. MishraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Titus Ponrathnam
Ravina Saini
Sofia Banu
Rakesh K. Mishra
Drosophila Hox genes induce melanized pseudo-tumors when misexpressed in hemocytes
description Abstract Hox genes are early determinants of cell identity along the anterior–posterior body axis across bilaterians. Several late non-homeotic functions of Hox genes have emerged in a variety of processes involved in organogenesis in several organisms, including mammals. Several studies have reported the misexpression of Hox genes in a variety of malignancies including acute myeloid leukemia. The Hox genes Dfd, Ubx, abd-A and Abd-B were overexpressed via the UAS-Gal4 system using Cg-Gal4, Lsp2-Gal4, He-Gal4 and HmlD3-Gal4 as specific drivers. Genetic interaction was tested by bringing overexpression lines in heterozygous mutant backgrounds of Polycomb and trithorax group factors. Larvae were visually scored for melanized bodies. Circulating hemocytes were quantified and tested for differentiation. Pupal lethality was assessed. Expression of Dfd, Ubx and abd-A, but not Abd-B in the hematopoietic compartment of Drosophila led to the appearance of circulating melanized bodies, an increase in cell number, cell-autonomous proliferation, and differentiation of hemocytes. Pupal lethality and melanized pseudo-tumors were suppressed in Psc 1 and esc 2 backgrounds while polycomb group member mutations Pc 1 and Su(z)12 3 and trithorax group member mutation TrlR 85 enhanced the phenotype. Dfd, Ubx and abd-A are leukemogenic. Mutations in Polycomb and trithorax group members modulate the leukemogenic phenotype. Our RNAseq of Cg-Gal4 > UAS-abd-A hemocytes may contain genes important to Hox gene induced leukemias.
format article
author Titus Ponrathnam
Ravina Saini
Sofia Banu
Rakesh K. Mishra
author_facet Titus Ponrathnam
Ravina Saini
Sofia Banu
Rakesh K. Mishra
author_sort Titus Ponrathnam
title Drosophila Hox genes induce melanized pseudo-tumors when misexpressed in hemocytes
title_short Drosophila Hox genes induce melanized pseudo-tumors when misexpressed in hemocytes
title_full Drosophila Hox genes induce melanized pseudo-tumors when misexpressed in hemocytes
title_fullStr Drosophila Hox genes induce melanized pseudo-tumors when misexpressed in hemocytes
title_full_unstemmed Drosophila Hox genes induce melanized pseudo-tumors when misexpressed in hemocytes
title_sort drosophila hox genes induce melanized pseudo-tumors when misexpressed in hemocytes
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/6117052a54ca414a9ef104e33571a0ee
work_keys_str_mv AT titusponrathnam drosophilahoxgenesinducemelanizedpseudotumorswhenmisexpressedinhemocytes
AT ravinasaini drosophilahoxgenesinducemelanizedpseudotumorswhenmisexpressedinhemocytes
AT sofiabanu drosophilahoxgenesinducemelanizedpseudotumorswhenmisexpressedinhemocytes
AT rakeshkmishra drosophilahoxgenesinducemelanizedpseudotumorswhenmisexpressedinhemocytes
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