Library-independent source tracking of fecal contamination in selected stations and tributaries of Laguna Lake, Philippines

Laguna Lake is the largest inland freshwater body in the Philippines. Although it is classified to be usable for agricultural and recreational purposes by the country's Department of Environment and Natural Resources (DENR), studies looking at lake ecology revealed severe fecal contamination wh...

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Autores principales:	Joseth Jermaine M. Abello, Gicelle T. Malajacan, Kevin L. Labrador, Mae Ashley G. Nacario, Luiza H. Galarion, Marie Christine M. Obusan, Windell L. Rivera
Formato:	article
Lenguaje:	EN
Publicado:	IWA Publishing 2021
Materias:	escherichia coli laguna lake microbial source tracking mitochondrial dna philippines Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/6117c14e1c3944f198533f53dbdb55bd
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spelling	oai:doaj.org-article:6117c14e1c3944f198533f53dbdb55bd2021-11-06T06:31:04ZLibrary-independent source tracking of fecal contamination in selected stations and tributaries of Laguna Lake, Philippines1477-89201996-782910.2166/wh.2021.058https://doaj.org/article/6117c14e1c3944f198533f53dbdb55bd2021-10-01T00:00:00Zhttp://jwh.iwaponline.com/content/19/5/846https://doaj.org/toc/1477-8920https://doaj.org/toc/1996-7829Laguna Lake is the largest inland freshwater body in the Philippines. Although it is classified to be usable for agricultural and recreational purposes by the country's Department of Environment and Natural Resources (DENR), studies looking at lake ecology revealed severe fecal contamination which contributes to the deterioration of water quality. Determining the sources of fecal contamination is necessary for lake protection and management. This study utilized a library-independent method of microbial source tracking (LIM-MST) to identify sources of fecal contamination in selected Laguna Lake stations and tributaries. Genetic markers of the host-associated Escherichia coli, heat-labile toxin (LTIIA) and heat-stable II (STII), were used to identify cattle and swine fecal contaminations, respectively. Meanwhile, human mitochondrial DNA (mtDNA) was used to identify human fecal contamination. Results identified the presence of agricultural and human fecal contamination in Laguna Lake Stations 1 and 5, Mangangate River, and Alabang River. The selected sites are known to be surrounded by residential and industrial complexes, and most of their discharges find their way into the lake. The identification of the specific sources of fecal contamination will guide management practices that aim to regulate the discharges in order to improve the water quality of Laguna Lake. HIGHLIGHTS The Escherichia coli LTIIa and STII genes, as well as human NADH mtDNA, are useful biomarkers for detecting fecal contamination in Laguna Lake.; Fecal contamination from agricultural and human origins can be detected in some lake stations and rivers near Laguna Lake.; As indicated by the high detection of LTIIa gene, fecal contamination in the sampling sites was mostly of cattle origin.;Joseth Jermaine M. AbelloGicelle T. MalajacanKevin L. LabradorMae Ashley G. NacarioLuiza H. GalarionMarie Christine M. ObusanWindell L. RiveraIWA Publishingarticleescherichia colilaguna lakemicrobial source trackingmitochondrial dnaphilippinesPublic aspects of medicineRA1-1270ENJournal of Water and Health, Vol 19, Iss 5, Pp 846-854 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	escherichia coli laguna lake microbial source tracking mitochondrial dna philippines Public aspects of medicine RA1-1270
spellingShingle	escherichia coli laguna lake microbial source tracking mitochondrial dna philippines Public aspects of medicine RA1-1270 Joseth Jermaine M. Abello Gicelle T. Malajacan Kevin L. Labrador Mae Ashley G. Nacario Luiza H. Galarion Marie Christine M. Obusan Windell L. Rivera Library-independent source tracking of fecal contamination in selected stations and tributaries of Laguna Lake, Philippines
description	Laguna Lake is the largest inland freshwater body in the Philippines. Although it is classified to be usable for agricultural and recreational purposes by the country's Department of Environment and Natural Resources (DENR), studies looking at lake ecology revealed severe fecal contamination which contributes to the deterioration of water quality. Determining the sources of fecal contamination is necessary for lake protection and management. This study utilized a library-independent method of microbial source tracking (LIM-MST) to identify sources of fecal contamination in selected Laguna Lake stations and tributaries. Genetic markers of the host-associated Escherichia coli, heat-labile toxin (LTIIA) and heat-stable II (STII), were used to identify cattle and swine fecal contaminations, respectively. Meanwhile, human mitochondrial DNA (mtDNA) was used to identify human fecal contamination. Results identified the presence of agricultural and human fecal contamination in Laguna Lake Stations 1 and 5, Mangangate River, and Alabang River. The selected sites are known to be surrounded by residential and industrial complexes, and most of their discharges find their way into the lake. The identification of the specific sources of fecal contamination will guide management practices that aim to regulate the discharges in order to improve the water quality of Laguna Lake. HIGHLIGHTS The Escherichia coli LTIIa and STII genes, as well as human NADH mtDNA, are useful biomarkers for detecting fecal contamination in Laguna Lake.; Fecal contamination from agricultural and human origins can be detected in some lake stations and rivers near Laguna Lake.; As indicated by the high detection of LTIIa gene, fecal contamination in the sampling sites was mostly of cattle origin.;
format	article
author	Joseth Jermaine M. Abello Gicelle T. Malajacan Kevin L. Labrador Mae Ashley G. Nacario Luiza H. Galarion Marie Christine M. Obusan Windell L. Rivera
author_facet	Joseth Jermaine M. Abello Gicelle T. Malajacan Kevin L. Labrador Mae Ashley G. Nacario Luiza H. Galarion Marie Christine M. Obusan Windell L. Rivera
author_sort	Joseth Jermaine M. Abello
title	Library-independent source tracking of fecal contamination in selected stations and tributaries of Laguna Lake, Philippines
title_short	Library-independent source tracking of fecal contamination in selected stations and tributaries of Laguna Lake, Philippines
title_full	Library-independent source tracking of fecal contamination in selected stations and tributaries of Laguna Lake, Philippines
title_fullStr	Library-independent source tracking of fecal contamination in selected stations and tributaries of Laguna Lake, Philippines
title_full_unstemmed	Library-independent source tracking of fecal contamination in selected stations and tributaries of Laguna Lake, Philippines
title_sort	library-independent source tracking of fecal contamination in selected stations and tributaries of laguna lake, philippines
publisher	IWA Publishing
publishDate	2021
url	https://doaj.org/article/6117c14e1c3944f198533f53dbdb55bd
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Library-independent source tracking of fecal contamination in selected stations and tributaries of Laguna Lake, Philippines

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