Enhanced cellular uptake of aminosilane-coated superparamagnetic iron oxide nanoparticles in mammalian cell lines

Xiao-Ming Zhu1, Yi-Xiang J Wang1, Ken Cham-Fai Leung2,3, Siu-Fung Lee2, Feng Zhao1, Da-Wei Wang2, Josie MY Lai4, Chao Wan4, Christopher HK Cheng4, Anil T Ahuja11Department of Imaging and Interventional Radiology, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SA...

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Autores principales:	Zhu XM, Wang YX, Leung KCF, Lee SF, Zhao F, Wang DW, Lai JMY, Wan C, Cheng CHK, Ahuja AT
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/611aa9badc4a4b0fa6b899b5a1799e0d
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spelling	oai:doaj.org-article:611aa9badc4a4b0fa6b899b5a1799e0d2021-12-02T05:10:27ZEnhanced cellular uptake of aminosilane-coated superparamagnetic iron oxide nanoparticles in mammalian cell lines1176-91141178-2013https://doaj.org/article/611aa9badc4a4b0fa6b899b5a1799e0d2012-02-01T00:00:00Zhttp://www.dovepress.com/enhanced-cellular-uptake-of-aminosilane-coated-superparamagnetic-iron--a9311https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Xiao-Ming Zhu1, Yi-Xiang J Wang1, Ken Cham-Fai Leung2,3, Siu-Fung Lee2, Feng Zhao1, Da-Wei Wang2, Josie MY Lai4, Chao Wan4, Christopher HK Cheng4, Anil T Ahuja11Department of Imaging and Interventional Radiology, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR; 2Institute of Molecular Functional Materials and Department of Chemistry, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR; 3Institute of Creativity and Department of Chemistry, The Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong SAR; 4School of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, NT, Hong KongPurpose: To compare the cellular uptake efficiency and cytotoxicity of aminosilane (SiO2-NH2)-coated superparamagnetic iron oxide (SPIO@SiO2-NH2) nanoparticles with three other types of SPIO nanoparticles coated with SiO2 (SPIO@SiO2), dextran (SPIO@dextran), or bare SPIO in mammalian cell lines.Materials and methods: Four types of monodispersed SPIO nanoparticles with a SPIO core size of 7 nm and an overall size in a range of 7&ndash;15 nm were synthesized. The mammalian cell lines of MCF-7, MDA-MB-231, HT-29, RAW264.7, L929, HepG2, PC-3, U-87 MG, and mouse mesenchymal stem cells (MSCs) were incubated with four types of SPIO nanoparticles for 24 hours in the serum-free culture medium Dulbecco&rsquo;s modified Eagle&rsquo;s medium (DMEM) with 4.5 &micro;g/mL iron concentration. The cellular uptake efficiencies of SPIO nanoparticles were compared by Prussian blue staining and intracellular iron quantification. In vitro magnetic resonance imaging of MSC pellets after SPIO labeling was performed at 3 T. The effect of each SPIO nanoparticle on the cell viability of RAW 264.7 (mouse monocyte/macrophage) cells was also evaluated.Results: Transmission electron microscopy demonstrated surface coating with SiO2-NH2, SiO2, and dextran prevented SPIO nanoparticle aggregation in DMEM culture medium. MCF-7, MDA-MB-231, and HT-29 cells failed to show notable iron uptake. For all the remaining six cell lines, Prussian blue staining and intracellular iron quantification demonstrated that SPIO@SiO2-NH2 nanoparticles had the highest cellular uptake efficiency. SPIO@SiO2-NH2, bare SPIO, and SPIO@dextran nanoparticles did not affect RAW 264.7 cell viability up to 200 &micro;g Fe/mL, while SPIO@SiO2 reduced RAW 264.7 cell viability from 10 to 200 &micro;g Fe/mL in a dose-dependent manner.Conclusion: Cellular uptake efficiency of SPIO nanoparticles depends on both the cell type and SPIO surface characteristics. Aminosilane surface coating enhanced the cellular uptake efficiency without inducing cytotoxicity in a number of cell lines.Keywords: magnetic nanoparticles, SPIO, iron oxide, surface coating, cellular uptakeZhu XMWang YXLeung KCFLee SFZhao FWang DWLai JMYWan CCheng CHKAhuja ATDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 953-964 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Zhu XM Wang YX Leung KCF Lee SF Zhao F Wang DW Lai JMY Wan C Cheng CHK Ahuja AT Enhanced cellular uptake of aminosilane-coated superparamagnetic iron oxide nanoparticles in mammalian cell lines
description	Xiao-Ming Zhu1, Yi-Xiang J Wang1, Ken Cham-Fai Leung2,3, Siu-Fung Lee2, Feng Zhao1, Da-Wei Wang2, Josie MY Lai4, Chao Wan4, Christopher HK Cheng4, Anil T Ahuja11Department of Imaging and Interventional Radiology, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR; 2Institute of Molecular Functional Materials and Department of Chemistry, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR; 3Institute of Creativity and Department of Chemistry, The Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong SAR; 4School of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, NT, Hong KongPurpose: To compare the cellular uptake efficiency and cytotoxicity of aminosilane (SiO2-NH2)-coated superparamagnetic iron oxide (SPIO@SiO2-NH2) nanoparticles with three other types of SPIO nanoparticles coated with SiO2 (SPIO@SiO2), dextran (SPIO@dextran), or bare SPIO in mammalian cell lines.Materials and methods: Four types of monodispersed SPIO nanoparticles with a SPIO core size of 7 nm and an overall size in a range of 7&ndash;15 nm were synthesized. The mammalian cell lines of MCF-7, MDA-MB-231, HT-29, RAW264.7, L929, HepG2, PC-3, U-87 MG, and mouse mesenchymal stem cells (MSCs) were incubated with four types of SPIO nanoparticles for 24 hours in the serum-free culture medium Dulbecco&rsquo;s modified Eagle&rsquo;s medium (DMEM) with 4.5 &micro;g/mL iron concentration. The cellular uptake efficiencies of SPIO nanoparticles were compared by Prussian blue staining and intracellular iron quantification. In vitro magnetic resonance imaging of MSC pellets after SPIO labeling was performed at 3 T. The effect of each SPIO nanoparticle on the cell viability of RAW 264.7 (mouse monocyte/macrophage) cells was also evaluated.Results: Transmission electron microscopy demonstrated surface coating with SiO2-NH2, SiO2, and dextran prevented SPIO nanoparticle aggregation in DMEM culture medium. MCF-7, MDA-MB-231, and HT-29 cells failed to show notable iron uptake. For all the remaining six cell lines, Prussian blue staining and intracellular iron quantification demonstrated that SPIO@SiO2-NH2 nanoparticles had the highest cellular uptake efficiency. SPIO@SiO2-NH2, bare SPIO, and SPIO@dextran nanoparticles did not affect RAW 264.7 cell viability up to 200 &micro;g Fe/mL, while SPIO@SiO2 reduced RAW 264.7 cell viability from 10 to 200 &micro;g Fe/mL in a dose-dependent manner.Conclusion: Cellular uptake efficiency of SPIO nanoparticles depends on both the cell type and SPIO surface characteristics. Aminosilane surface coating enhanced the cellular uptake efficiency without inducing cytotoxicity in a number of cell lines.Keywords: magnetic nanoparticles, SPIO, iron oxide, surface coating, cellular uptake
format	article
author	Zhu XM Wang YX Leung KCF Lee SF Zhao F Wang DW Lai JMY Wan C Cheng CHK Ahuja AT
author_facet	Zhu XM Wang YX Leung KCF Lee SF Zhao F Wang DW Lai JMY Wan C Cheng CHK Ahuja AT
author_sort	Zhu XM
title	Enhanced cellular uptake of aminosilane-coated superparamagnetic iron oxide nanoparticles in mammalian cell lines
title_short	Enhanced cellular uptake of aminosilane-coated superparamagnetic iron oxide nanoparticles in mammalian cell lines
title_full	Enhanced cellular uptake of aminosilane-coated superparamagnetic iron oxide nanoparticles in mammalian cell lines
title_fullStr	Enhanced cellular uptake of aminosilane-coated superparamagnetic iron oxide nanoparticles in mammalian cell lines
title_full_unstemmed	Enhanced cellular uptake of aminosilane-coated superparamagnetic iron oxide nanoparticles in mammalian cell lines
title_sort	enhanced cellular uptake of aminosilane-coated superparamagnetic iron oxide nanoparticles in mammalian cell lines
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/611aa9badc4a4b0fa6b899b5a1799e0d
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Enhanced cellular uptake of aminosilane-coated superparamagnetic iron oxide nanoparticles in mammalian cell lines

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