Passive repetitive stretching is associated with greater muscle mass and cross-sectional area in the sarcopenic muscle

Abstract Mechanical stimulation has benefits for muscle mass and function. Passive stretching is widely performed in clinical rehabilitation medicine. However, the hypertrophic effects of passive repetitive stretching on senescent skeletal muscles against muscle atrophy remain unknown. We used senes...

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Autores principales:	Yumin Wang, Satoshi Ikeda, Katsunori Ikoma
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/611d4ca8244c43b39912db513ef2e68a
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spelling	oai:doaj.org-article:611d4ca8244c43b39912db513ef2e68a2021-12-02T16:24:56ZPassive repetitive stretching is associated with greater muscle mass and cross-sectional area in the sarcopenic muscle10.1038/s41598-021-94709-02045-2322https://doaj.org/article/611d4ca8244c43b39912db513ef2e68a2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94709-0https://doaj.org/toc/2045-2322Abstract Mechanical stimulation has benefits for muscle mass and function. Passive stretching is widely performed in clinical rehabilitation medicine. However, the hypertrophic effects of passive repetitive stretching on senescent skeletal muscles against muscle atrophy remain unknown. We used senescence-accelerated model SAM-P8 mice. The gastrocnemius muscle was passively repetitive stretched by manual ankle dorsiflexion for 15 min, 5 days a week for 2 weeks under deep anesthesia. We examined the effects of passive stretching on muscle mass, myofiber cross-sectional area, muscle fiber type composition, satellite cell and myonuclei content, signaling pathways involved in muscle protein synthesis, and myogenic regulatory factors. The gastrocnemius muscle weight and fiber cross-sectional area of the stretched side was found greater compared with that of the unstretched side. Passive repetitive stretching increased the mRNA expression level of Akt, p70S6K, 4E-BP1, Myf5, myogenin, MuRF1.The phosphorylation level of p70S6K significantly increased in the stretched muscles, whereas of Akt and 4E-BP1 remained unchanged, compared to the unstretched side. The Pax7+ cells and myonuclei content did not differ between the stretched and unstretched muscles. These findings suggest that the hypertrophic or suppressed atrophic observation in the stretched muscles are mainly attributable to the protein turnover provoked by stretching. These findings are applicable to clinical muscle strengthening and sarcopenia prevention.Yumin WangSatoshi IkedaKatsunori IkomaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yumin Wang Satoshi Ikeda Katsunori Ikoma Passive repetitive stretching is associated with greater muscle mass and cross-sectional area in the sarcopenic muscle
description	Abstract Mechanical stimulation has benefits for muscle mass and function. Passive stretching is widely performed in clinical rehabilitation medicine. However, the hypertrophic effects of passive repetitive stretching on senescent skeletal muscles against muscle atrophy remain unknown. We used senescence-accelerated model SAM-P8 mice. The gastrocnemius muscle was passively repetitive stretched by manual ankle dorsiflexion for 15 min, 5 days a week for 2 weeks under deep anesthesia. We examined the effects of passive stretching on muscle mass, myofiber cross-sectional area, muscle fiber type composition, satellite cell and myonuclei content, signaling pathways involved in muscle protein synthesis, and myogenic regulatory factors. The gastrocnemius muscle weight and fiber cross-sectional area of the stretched side was found greater compared with that of the unstretched side. Passive repetitive stretching increased the mRNA expression level of Akt, p70S6K, 4E-BP1, Myf5, myogenin, MuRF1.The phosphorylation level of p70S6K significantly increased in the stretched muscles, whereas of Akt and 4E-BP1 remained unchanged, compared to the unstretched side. The Pax7+ cells and myonuclei content did not differ between the stretched and unstretched muscles. These findings suggest that the hypertrophic or suppressed atrophic observation in the stretched muscles are mainly attributable to the protein turnover provoked by stretching. These findings are applicable to clinical muscle strengthening and sarcopenia prevention.
format	article
author	Yumin Wang Satoshi Ikeda Katsunori Ikoma
author_facet	Yumin Wang Satoshi Ikeda Katsunori Ikoma
author_sort	Yumin Wang
title	Passive repetitive stretching is associated with greater muscle mass and cross-sectional area in the sarcopenic muscle
title_short	Passive repetitive stretching is associated with greater muscle mass and cross-sectional area in the sarcopenic muscle
title_full	Passive repetitive stretching is associated with greater muscle mass and cross-sectional area in the sarcopenic muscle
title_fullStr	Passive repetitive stretching is associated with greater muscle mass and cross-sectional area in the sarcopenic muscle
title_full_unstemmed	Passive repetitive stretching is associated with greater muscle mass and cross-sectional area in the sarcopenic muscle
title_sort	passive repetitive stretching is associated with greater muscle mass and cross-sectional area in the sarcopenic muscle
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/611d4ca8244c43b39912db513ef2e68a
work_keys_str_mv	AT yuminwang passiverepetitivestretchingisassociatedwithgreatermusclemassandcrosssectionalareainthesarcopenicmuscle AT satoshiikeda passiverepetitivestretchingisassociatedwithgreatermusclemassandcrosssectionalareainthesarcopenicmuscle AT katsunoriikoma passiverepetitivestretchingisassociatedwithgreatermusclemassandcrosssectionalareainthesarcopenicmuscle
_version_	1718384112967876608

Passive repetitive stretching is associated with greater muscle mass and cross-sectional area in the sarcopenic muscle

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