Conjunctiva-associated lymphoid tissue (CALT) reactions to antiglaucoma prostaglandins with or without BAK-preservative in rabbit acute toxicity study.

Conjunctiva-associated lymphoid tissue (CALT) is closely associated with ocular surface immunity. This study investigated the effects of antiglaucoma prostaglandin analogs with or without benzalkonium chloride (BAK) preservative on organized CALT using an acute toxic model. A total of 48 albino rabb...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hong Liang, Christophe Baudouin, Antoine Labbe, Luisa Riancho, Françoise Brignole-Baudouin
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/611f407605274c53a0ea8ce7e7db8bac
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:611f407605274c53a0ea8ce7e7db8bac
record_format	dspace
spelling	oai:doaj.org-article:611f407605274c53a0ea8ce7e7db8bac2021-11-18T07:24:51ZConjunctiva-associated lymphoid tissue (CALT) reactions to antiglaucoma prostaglandins with or without BAK-preservative in rabbit acute toxicity study.1932-620310.1371/journal.pone.0033913https://doaj.org/article/611f407605274c53a0ea8ce7e7db8bac2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22442734/?tool=EBIhttps://doaj.org/toc/1932-6203Conjunctiva-associated lymphoid tissue (CALT) is closely associated with ocular surface immunity. This study investigated the effects of antiglaucoma prostaglandin analogs with or without benzalkonium chloride (BAK) preservative on organized CALT using an acute toxic model. A total of 48 albino rabbits were used and seven groups of treatments were constituted. Solutions (50 µl) of PBS, 0.02%BAK, (0.02%BAK+)latanoprost, (0.015%BAK+)travoprost, (0.005%BAK+)bimatoprost, (BAK-free)travoprost preserved with the SofZia® system or (BAK-free)tafluprost were instilled 15 times at 5-min intervals in both eyes. CALT changes were analyzed using in vivo confocal microscopy (IVCM), immunohistology in cryosections for detecting MUC-5AC+ mucocytes and CD45+ hematopoietic cells. Antiglaucoma eye drops stimulated inflammatory cell infiltration in the CALT, and seemed to be primarily related to the concentration of their BAK content. The CALT reaction after instillation of BAK-containing eye drops was characterized by inflammatory cell infiltration in the dome and intrafollicular layers and by cell circulation inside the lymph vessels. CD45 was strongly expressed in the CALT after instillation of all BAK-containing solutions at 4 h and decreased at 24 h. The number of MUC-5AC+ mucocytes around the CALT structure decreased dramatically after instillation of BAK-containing solutions. This study showed for the first time the in vivo aspect of rabbit CALT after toxic stimuli, confirming the concentration-dependent toxic effects of BAK. IVCM-CALT analysis could be a pertinent tool in the future for understanding the immunotoxicologic challenges in the ocular surface and would provide useful criteria for evaluating newly developed eye drops.Hong LiangChristophe BaudouinAntoine LabbeLuisa RianchoFrançoise Brignole-BaudouinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 3, p e33913 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hong Liang Christophe Baudouin Antoine Labbe Luisa Riancho Françoise Brignole-Baudouin Conjunctiva-associated lymphoid tissue (CALT) reactions to antiglaucoma prostaglandins with or without BAK-preservative in rabbit acute toxicity study.
description	Conjunctiva-associated lymphoid tissue (CALT) is closely associated with ocular surface immunity. This study investigated the effects of antiglaucoma prostaglandin analogs with or without benzalkonium chloride (BAK) preservative on organized CALT using an acute toxic model. A total of 48 albino rabbits were used and seven groups of treatments were constituted. Solutions (50 µl) of PBS, 0.02%BAK, (0.02%BAK+)latanoprost, (0.015%BAK+)travoprost, (0.005%BAK+)bimatoprost, (BAK-free)travoprost preserved with the SofZia® system or (BAK-free)tafluprost were instilled 15 times at 5-min intervals in both eyes. CALT changes were analyzed using in vivo confocal microscopy (IVCM), immunohistology in cryosections for detecting MUC-5AC+ mucocytes and CD45+ hematopoietic cells. Antiglaucoma eye drops stimulated inflammatory cell infiltration in the CALT, and seemed to be primarily related to the concentration of their BAK content. The CALT reaction after instillation of BAK-containing eye drops was characterized by inflammatory cell infiltration in the dome and intrafollicular layers and by cell circulation inside the lymph vessels. CD45 was strongly expressed in the CALT after instillation of all BAK-containing solutions at 4 h and decreased at 24 h. The number of MUC-5AC+ mucocytes around the CALT structure decreased dramatically after instillation of BAK-containing solutions. This study showed for the first time the in vivo aspect of rabbit CALT after toxic stimuli, confirming the concentration-dependent toxic effects of BAK. IVCM-CALT analysis could be a pertinent tool in the future for understanding the immunotoxicologic challenges in the ocular surface and would provide useful criteria for evaluating newly developed eye drops.
format	article
author	Hong Liang Christophe Baudouin Antoine Labbe Luisa Riancho Françoise Brignole-Baudouin
author_facet	Hong Liang Christophe Baudouin Antoine Labbe Luisa Riancho Françoise Brignole-Baudouin
author_sort	Hong Liang
title	Conjunctiva-associated lymphoid tissue (CALT) reactions to antiglaucoma prostaglandins with or without BAK-preservative in rabbit acute toxicity study.
title_short	Conjunctiva-associated lymphoid tissue (CALT) reactions to antiglaucoma prostaglandins with or without BAK-preservative in rabbit acute toxicity study.
title_full	Conjunctiva-associated lymphoid tissue (CALT) reactions to antiglaucoma prostaglandins with or without BAK-preservative in rabbit acute toxicity study.
title_fullStr	Conjunctiva-associated lymphoid tissue (CALT) reactions to antiglaucoma prostaglandins with or without BAK-preservative in rabbit acute toxicity study.
title_full_unstemmed	Conjunctiva-associated lymphoid tissue (CALT) reactions to antiglaucoma prostaglandins with or without BAK-preservative in rabbit acute toxicity study.
title_sort	conjunctiva-associated lymphoid tissue (calt) reactions to antiglaucoma prostaglandins with or without bak-preservative in rabbit acute toxicity study.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/611f407605274c53a0ea8ce7e7db8bac
work_keys_str_mv	AT hongliang conjunctivaassociatedlymphoidtissuecaltreactionstoantiglaucomaprostaglandinswithorwithoutbakpreservativeinrabbitacutetoxicitystudy AT christophebaudouin conjunctivaassociatedlymphoidtissuecaltreactionstoantiglaucomaprostaglandinswithorwithoutbakpreservativeinrabbitacutetoxicitystudy AT antoinelabbe conjunctivaassociatedlymphoidtissuecaltreactionstoantiglaucomaprostaglandinswithorwithoutbakpreservativeinrabbitacutetoxicitystudy AT luisariancho conjunctivaassociatedlymphoidtissuecaltreactionstoantiglaucomaprostaglandinswithorwithoutbakpreservativeinrabbitacutetoxicitystudy AT francoisebrignolebaudouin conjunctivaassociatedlymphoidtissuecaltreactionstoantiglaucomaprostaglandinswithorwithoutbakpreservativeinrabbitacutetoxicitystudy
_version_	1718423477558444032

Conjunctiva-associated lymphoid tissue (CALT) reactions to antiglaucoma prostaglandins with or without BAK-preservative in rabbit acute toxicity study.

Ejemplares similares