Adiponectin regulates BMSC osteogenic differentiation and osteogenesis through the Wnt/β-catenin pathway

Abstract Recent studies have demonstrated the stimulatory effects of adiponectin on bone formation, but the mechanism underlying these effects remains unclear. The Wnt/β-catenin pathway, one of the most important pathways in osteogenesis, has rarely been associated with the osteogenic effects of adi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yiyao Wang, Xiaohui Zhang, Jun Shao, Hanghang Liu, Xian Liu, En Luo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/61202b641e8f4e149a7762f6f389e4f2
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:61202b641e8f4e149a7762f6f389e4f2
record_format	dspace
spelling	oai:doaj.org-article:61202b641e8f4e149a7762f6f389e4f22021-12-02T12:30:52ZAdiponectin regulates BMSC osteogenic differentiation and osteogenesis through the Wnt/β-catenin pathway10.1038/s41598-017-03899-z2045-2322https://doaj.org/article/61202b641e8f4e149a7762f6f389e4f22017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03899-zhttps://doaj.org/toc/2045-2322Abstract Recent studies have demonstrated the stimulatory effects of adiponectin on bone formation, but the mechanism underlying these effects remains unclear. The Wnt/β-catenin pathway, one of the most important pathways in osteogenesis, has rarely been associated with the osteogenic effects of adiponectin in previous studies. The present study was designed to investigate the effects of adiponectin on bone mesenchymal stem cell (BMSC) osteogenic differentiation and bone formation through the Wnt/β-catenin pathway. We detected adiponectin receptor expression in BMSCs, constructed a recombinant adenovirus containing the human adiponectin gene, and then used the adenovirus to transfect BMSCs in vitro or injected the adenovirus into bone defect areas in animal models. Wnt/β-catenin pathway and osteogenesis were detected by real-time PCR, western blotting, immunofluorescence, HE staining and micro-CT. In both our in vivo and in vitro experiments, we detected higher gene and protein expression levels of the Wnt/β-catenin pathway-related factors β-catenin and cyclinD1 in adiponectin transgenic BMSCs and rats. Similar results were noted regarding the gene and protein expression levels of osteogenesis-related genes. In addition, more new bone formation was observed in the adiponectin-treated groups. Our results indicate that adiponectin could facilitate BMSC osteogenic differentiation and osteogenesis, and the Wnt/β-catenin pathway was involved in the osteogenic effect of adiponectin.Yiyao WangXiaohui ZhangJun ShaoHanghang LiuXian LiuEn LuoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yiyao Wang Xiaohui Zhang Jun Shao Hanghang Liu Xian Liu En Luo Adiponectin regulates BMSC osteogenic differentiation and osteogenesis through the Wnt/β-catenin pathway
description	Abstract Recent studies have demonstrated the stimulatory effects of adiponectin on bone formation, but the mechanism underlying these effects remains unclear. The Wnt/β-catenin pathway, one of the most important pathways in osteogenesis, has rarely been associated with the osteogenic effects of adiponectin in previous studies. The present study was designed to investigate the effects of adiponectin on bone mesenchymal stem cell (BMSC) osteogenic differentiation and bone formation through the Wnt/β-catenin pathway. We detected adiponectin receptor expression in BMSCs, constructed a recombinant adenovirus containing the human adiponectin gene, and then used the adenovirus to transfect BMSCs in vitro or injected the adenovirus into bone defect areas in animal models. Wnt/β-catenin pathway and osteogenesis were detected by real-time PCR, western blotting, immunofluorescence, HE staining and micro-CT. In both our in vivo and in vitro experiments, we detected higher gene and protein expression levels of the Wnt/β-catenin pathway-related factors β-catenin and cyclinD1 in adiponectin transgenic BMSCs and rats. Similar results were noted regarding the gene and protein expression levels of osteogenesis-related genes. In addition, more new bone formation was observed in the adiponectin-treated groups. Our results indicate that adiponectin could facilitate BMSC osteogenic differentiation and osteogenesis, and the Wnt/β-catenin pathway was involved in the osteogenic effect of adiponectin.
format	article
author	Yiyao Wang Xiaohui Zhang Jun Shao Hanghang Liu Xian Liu En Luo
author_facet	Yiyao Wang Xiaohui Zhang Jun Shao Hanghang Liu Xian Liu En Luo
author_sort	Yiyao Wang
title	Adiponectin regulates BMSC osteogenic differentiation and osteogenesis through the Wnt/β-catenin pathway
title_short	Adiponectin regulates BMSC osteogenic differentiation and osteogenesis through the Wnt/β-catenin pathway
title_full	Adiponectin regulates BMSC osteogenic differentiation and osteogenesis through the Wnt/β-catenin pathway
title_fullStr	Adiponectin regulates BMSC osteogenic differentiation and osteogenesis through the Wnt/β-catenin pathway
title_full_unstemmed	Adiponectin regulates BMSC osteogenic differentiation and osteogenesis through the Wnt/β-catenin pathway
title_sort	adiponectin regulates bmsc osteogenic differentiation and osteogenesis through the wnt/β-catenin pathway
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/61202b641e8f4e149a7762f6f389e4f2
work_keys_str_mv	AT yiyaowang adiponectinregulatesbmscosteogenicdifferentiationandosteogenesisthroughthewntbcateninpathway AT xiaohuizhang adiponectinregulatesbmscosteogenicdifferentiationandosteogenesisthroughthewntbcateninpathway AT junshao adiponectinregulatesbmscosteogenicdifferentiationandosteogenesisthroughthewntbcateninpathway AT hanghangliu adiponectinregulatesbmscosteogenicdifferentiationandosteogenesisthroughthewntbcateninpathway AT xianliu adiponectinregulatesbmscosteogenicdifferentiationandosteogenesisthroughthewntbcateninpathway AT enluo adiponectinregulatesbmscosteogenicdifferentiationandosteogenesisthroughthewntbcateninpathway
_version_	1718394281235841024

Adiponectin regulates BMSC osteogenic differentiation and osteogenesis through the Wnt/β-catenin pathway

Ejemplares similares