Assessment of flow within developing chicken vasculature and biofabricated vascularized tissues using multimodal imaging techniques

Abstract Fluid flow shear stresses are strong regulators for directing the organization of vascular networks. Knowledge of structural and flow dynamics information within complex vasculature is essential for tuning the vascular organization within engineered tissues, by manipulating flows. However,...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Prasanna Padmanaban, Ata Chizari, Tom Knop, Jiena Zhang, Vasileios D. Trikalitis, Bart Koopman, Wiendelt Steenbergen, Jeroen Rouwkema
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/612cedac0bab40118651345043a33cd6
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:612cedac0bab40118651345043a33cd6
record_format	dspace
spelling	oai:doaj.org-article:612cedac0bab40118651345043a33cd62021-12-02T18:49:53ZAssessment of flow within developing chicken vasculature and biofabricated vascularized tissues using multimodal imaging techniques10.1038/s41598-021-97008-w2045-2322https://doaj.org/article/612cedac0bab40118651345043a33cd62021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97008-whttps://doaj.org/toc/2045-2322Abstract Fluid flow shear stresses are strong regulators for directing the organization of vascular networks. Knowledge of structural and flow dynamics information within complex vasculature is essential for tuning the vascular organization within engineered tissues, by manipulating flows. However, reported investigations of vascular organization and their associated flow dynamics within complex vasculature over time are limited, due to limitations in the available physiological pre-clinical models, and the optical inaccessibility and aseptic nature of these models. Here, we developed laser speckle contrast imaging (LSCI) and side-stream dark field microscopy (SDF) systems to map the vascular organization, spatio-temporal blood flow fluctuations as well as erythrocytes movements within individual blood vessels of developing chick embryo, cultured within an artificial eggshell system. By combining imaging data and computational simulations, we estimated fluid flow shear stresses within multiscale vasculature of varying complexity. Furthermore, we demonstrated the LSCI compatibility with bioengineered perfusable muscle tissue constructs, fabricated via molding techniques. The presented application of LSCI and SDF on perfusable tissues enables us to study the flow perfusion effects in a non-invasive fashion. The gained knowledge can help to use fluid perfusion in order to tune and control multiscale vascular organization within engineered tissues.Prasanna PadmanabanAta ChizariTom KnopJiena ZhangVasileios D. TrikalitisBart KoopmanWiendelt SteenbergenJeroen RouwkemaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Prasanna Padmanaban Ata Chizari Tom Knop Jiena Zhang Vasileios D. Trikalitis Bart Koopman Wiendelt Steenbergen Jeroen Rouwkema Assessment of flow within developing chicken vasculature and biofabricated vascularized tissues using multimodal imaging techniques
description	Abstract Fluid flow shear stresses are strong regulators for directing the organization of vascular networks. Knowledge of structural and flow dynamics information within complex vasculature is essential for tuning the vascular organization within engineered tissues, by manipulating flows. However, reported investigations of vascular organization and their associated flow dynamics within complex vasculature over time are limited, due to limitations in the available physiological pre-clinical models, and the optical inaccessibility and aseptic nature of these models. Here, we developed laser speckle contrast imaging (LSCI) and side-stream dark field microscopy (SDF) systems to map the vascular organization, spatio-temporal blood flow fluctuations as well as erythrocytes movements within individual blood vessels of developing chick embryo, cultured within an artificial eggshell system. By combining imaging data and computational simulations, we estimated fluid flow shear stresses within multiscale vasculature of varying complexity. Furthermore, we demonstrated the LSCI compatibility with bioengineered perfusable muscle tissue constructs, fabricated via molding techniques. The presented application of LSCI and SDF on perfusable tissues enables us to study the flow perfusion effects in a non-invasive fashion. The gained knowledge can help to use fluid perfusion in order to tune and control multiscale vascular organization within engineered tissues.
format	article
author	Prasanna Padmanaban Ata Chizari Tom Knop Jiena Zhang Vasileios D. Trikalitis Bart Koopman Wiendelt Steenbergen Jeroen Rouwkema
author_facet	Prasanna Padmanaban Ata Chizari Tom Knop Jiena Zhang Vasileios D. Trikalitis Bart Koopman Wiendelt Steenbergen Jeroen Rouwkema
author_sort	Prasanna Padmanaban
title	Assessment of flow within developing chicken vasculature and biofabricated vascularized tissues using multimodal imaging techniques
title_short	Assessment of flow within developing chicken vasculature and biofabricated vascularized tissues using multimodal imaging techniques
title_full	Assessment of flow within developing chicken vasculature and biofabricated vascularized tissues using multimodal imaging techniques
title_fullStr	Assessment of flow within developing chicken vasculature and biofabricated vascularized tissues using multimodal imaging techniques
title_full_unstemmed	Assessment of flow within developing chicken vasculature and biofabricated vascularized tissues using multimodal imaging techniques
title_sort	assessment of flow within developing chicken vasculature and biofabricated vascularized tissues using multimodal imaging techniques
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/612cedac0bab40118651345043a33cd6
work_keys_str_mv	AT prasannapadmanaban assessmentofflowwithindevelopingchickenvasculatureandbiofabricatedvascularizedtissuesusingmultimodalimagingtechniques AT atachizari assessmentofflowwithindevelopingchickenvasculatureandbiofabricatedvascularizedtissuesusingmultimodalimagingtechniques AT tomknop assessmentofflowwithindevelopingchickenvasculatureandbiofabricatedvascularizedtissuesusingmultimodalimagingtechniques AT jienazhang assessmentofflowwithindevelopingchickenvasculatureandbiofabricatedvascularizedtissuesusingmultimodalimagingtechniques AT vasileiosdtrikalitis assessmentofflowwithindevelopingchickenvasculatureandbiofabricatedvascularizedtissuesusingmultimodalimagingtechniques AT bartkoopman assessmentofflowwithindevelopingchickenvasculatureandbiofabricatedvascularizedtissuesusingmultimodalimagingtechniques AT wiendeltsteenbergen assessmentofflowwithindevelopingchickenvasculatureandbiofabricatedvascularizedtissuesusingmultimodalimagingtechniques AT jeroenrouwkema assessmentofflowwithindevelopingchickenvasculatureandbiofabricatedvascularizedtissuesusingmultimodalimagingtechniques
_version_	1718377506543763456

Assessment of flow within developing chicken vasculature and biofabricated vascularized tissues using multimodal imaging techniques

Ejemplares similares