IgE-binding residues analysis of the house dust mite allergen Der p 23

Abstract House dust mites (HDMs) are one of the major causes of allergies in the world. The group 23 allergen, Der p 23, from Dermatophagoides pteronyssinus, is a major allergen amongst HDM-sensitized individuals. This study aims to determine the specific immunoglobulin E (sIgE) binding frequency an...

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Autores principales:	Sze Lei Pang, Sri Anusha Matta, Yang Yie Sio, Yu Ting Ng, Yee-How Say, Chyan Leong Ng, Fook Tim Chew
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6142696145e1406fb4c394791aa06c30
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spelling	oai:doaj.org-article:6142696145e1406fb4c394791aa06c302021-12-02T14:01:19ZIgE-binding residues analysis of the house dust mite allergen Der p 2310.1038/s41598-020-79820-y2045-2322https://doaj.org/article/6142696145e1406fb4c394791aa06c302021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79820-yhttps://doaj.org/toc/2045-2322Abstract House dust mites (HDMs) are one of the major causes of allergies in the world. The group 23 allergen, Der p 23, from Dermatophagoides pteronyssinus, is a major allergen amongst HDM-sensitized individuals. This study aims to determine the specific immunoglobulin E (sIgE) binding frequency and IgE-binding residues of recombinant Der p 23 (rDer p 23) allergen amongst a cohort of consecutive atopic individuals in a tropical region. We performed site-directed mutagenesis and carried out immuno-dot blot assays using 65 atopic sera. The immuno-dot blot assays results indicated that the two residues K44 and E46 which are located at the N-terminal region are the major IgE-binding residues. The rDerp-23 sIgE titers are strongly correlated to the number of IgE-binding residues for rDer p 23 (P < 0.001). Atopic individuals who were only sensitized to HDM have a significantly higher number of IgE-binding residues than the individuals who were polysensitized to HDM and other crude allergens (P < 0.05). Individuals with allergic multimorbidity and moderate-to-severe allergic rhinitis also have a higher number of IgE-binding residues compared to those with single allergic disease and mild allergic rhinitis. The results prompt us to hypothesize that the individuals who have a higher number of IgE-binding residues may face a bigger challenge to be treated through immunotherapy due to the complexity in designing an effective hypoallergen with a high number of IgE-binding residues. We propose that the development of a refined molecular diagnostic assay, which includes alanine substitution of surface-exposed residues could be a more precise diagnostic strategy to identify all the IgE-binding residues of a major allergen for an atopic individual and the development could be another new dimension in allergy diagnosis and allergen immunotherapy treatment.Sze Lei PangSri Anusha MattaYang Yie SioYu Ting NgYee-How SayChyan Leong NgFook Tim ChewNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sze Lei Pang Sri Anusha Matta Yang Yie Sio Yu Ting Ng Yee-How Say Chyan Leong Ng Fook Tim Chew IgE-binding residues analysis of the house dust mite allergen Der p 23
description	Abstract House dust mites (HDMs) are one of the major causes of allergies in the world. The group 23 allergen, Der p 23, from Dermatophagoides pteronyssinus, is a major allergen amongst HDM-sensitized individuals. This study aims to determine the specific immunoglobulin E (sIgE) binding frequency and IgE-binding residues of recombinant Der p 23 (rDer p 23) allergen amongst a cohort of consecutive atopic individuals in a tropical region. We performed site-directed mutagenesis and carried out immuno-dot blot assays using 65 atopic sera. The immuno-dot blot assays results indicated that the two residues K44 and E46 which are located at the N-terminal region are the major IgE-binding residues. The rDerp-23 sIgE titers are strongly correlated to the number of IgE-binding residues for rDer p 23 (P < 0.001). Atopic individuals who were only sensitized to HDM have a significantly higher number of IgE-binding residues than the individuals who were polysensitized to HDM and other crude allergens (P < 0.05). Individuals with allergic multimorbidity and moderate-to-severe allergic rhinitis also have a higher number of IgE-binding residues compared to those with single allergic disease and mild allergic rhinitis. The results prompt us to hypothesize that the individuals who have a higher number of IgE-binding residues may face a bigger challenge to be treated through immunotherapy due to the complexity in designing an effective hypoallergen with a high number of IgE-binding residues. We propose that the development of a refined molecular diagnostic assay, which includes alanine substitution of surface-exposed residues could be a more precise diagnostic strategy to identify all the IgE-binding residues of a major allergen for an atopic individual and the development could be another new dimension in allergy diagnosis and allergen immunotherapy treatment.
format	article
author	Sze Lei Pang Sri Anusha Matta Yang Yie Sio Yu Ting Ng Yee-How Say Chyan Leong Ng Fook Tim Chew
author_facet	Sze Lei Pang Sri Anusha Matta Yang Yie Sio Yu Ting Ng Yee-How Say Chyan Leong Ng Fook Tim Chew
author_sort	Sze Lei Pang
title	IgE-binding residues analysis of the house dust mite allergen Der p 23
title_short	IgE-binding residues analysis of the house dust mite allergen Der p 23
title_full	IgE-binding residues analysis of the house dust mite allergen Der p 23
title_fullStr	IgE-binding residues analysis of the house dust mite allergen Der p 23
title_full_unstemmed	IgE-binding residues analysis of the house dust mite allergen Der p 23
title_sort	ige-binding residues analysis of the house dust mite allergen der p 23
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6142696145e1406fb4c394791aa06c30
work_keys_str_mv	AT szeleipang igebindingresiduesanalysisofthehousedustmiteallergenderp23 AT srianushamatta igebindingresiduesanalysisofthehousedustmiteallergenderp23 AT yangyiesio igebindingresiduesanalysisofthehousedustmiteallergenderp23 AT yutingng igebindingresiduesanalysisofthehousedustmiteallergenderp23 AT yeehowsay igebindingresiduesanalysisofthehousedustmiteallergenderp23 AT chyanleongng igebindingresiduesanalysisofthehousedustmiteallergenderp23 AT fooktimchew igebindingresiduesanalysisofthehousedustmiteallergenderp23
_version_	1718392202550312960

IgE-binding residues analysis of the house dust mite allergen Der p 23

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