Development of a New Screen-Printed Transducer for the Electrochemical Detection of Thiram

A new transducer based on a screen-printed carbon electrode has been developed for the quantification of thiram. Detection of this fungicide is based on the performance of two enzymes: (1) aldehyde dehydrogenase catalyzes the aldehyde oxidation using NAD<sup>+</sup> as a cofactor and sim...

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Autores principales:	David Ibáñez, Daniel Izquierdo-Bote, María Begoña González-García, David Hernández-Santos, Pablo Fanjul-Bolado
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	thiram aldehyde dehydrogenase diaphorase screen-printed electrodes (SPEs) transducer Biochemistry QD415-436
Acceso en línea:	https://doaj.org/article/6144793019aa4ee69223102f6ccf4855
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spelling	oai:doaj.org-article:6144793019aa4ee69223102f6ccf48552021-11-25T17:13:32ZDevelopment of a New Screen-Printed Transducer for the Electrochemical Detection of Thiram10.3390/chemosensors91103032227-9040https://doaj.org/article/6144793019aa4ee69223102f6ccf48552021-10-01T00:00:00Zhttps://www.mdpi.com/2227-9040/9/11/303https://doaj.org/toc/2227-9040A new transducer based on a screen-printed carbon electrode has been developed for the quantification of thiram. Detection of this fungicide is based on the performance of two enzymes: (1) aldehyde dehydrogenase catalyzes the aldehyde oxidation using NAD<sup>+</sup> as a cofactor and simultaneously, (2) diaphorase reoxidizes the NADH formed in the first enzymatic process due to the presence of hexacyanoferrate(III) which is reduced to hexacyanoferrate(II). Taking into account that aldehyde dehydrogenase is inhibited by thiram, the current decreases with pesticide concentration and thiram can be electrochemically quantified below legal limits. The transducer proposed in this work involves the modification of the carbon WE with the co-factors (NAD<sup>+</sup> and hexacyanoferrate(III)) required in the enzymatic system. The new device employed in this work allows the detection of 0.09 ppm thiram, a concentration below legal limits (Maximum Residue Limits 0.1–10 ppm).David IbáñezDaniel Izquierdo-BoteMaría Begoña González-GarcíaDavid Hernández-SantosPablo Fanjul-BoladoMDPI AGarticlethiramaldehyde dehydrogenasediaphorasescreen-printed electrodes (SPEs)transducerBiochemistryQD415-436ENChemosensors, Vol 9, Iss 303, p 303 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	thiram aldehyde dehydrogenase diaphorase screen-printed electrodes (SPEs) transducer Biochemistry QD415-436
spellingShingle	thiram aldehyde dehydrogenase diaphorase screen-printed electrodes (SPEs) transducer Biochemistry QD415-436 David Ibáñez Daniel Izquierdo-Bote María Begoña González-García David Hernández-Santos Pablo Fanjul-Bolado Development of a New Screen-Printed Transducer for the Electrochemical Detection of Thiram
description	A new transducer based on a screen-printed carbon electrode has been developed for the quantification of thiram. Detection of this fungicide is based on the performance of two enzymes: (1) aldehyde dehydrogenase catalyzes the aldehyde oxidation using NAD<sup>+</sup> as a cofactor and simultaneously, (2) diaphorase reoxidizes the NADH formed in the first enzymatic process due to the presence of hexacyanoferrate(III) which is reduced to hexacyanoferrate(II). Taking into account that aldehyde dehydrogenase is inhibited by thiram, the current decreases with pesticide concentration and thiram can be electrochemically quantified below legal limits. The transducer proposed in this work involves the modification of the carbon WE with the co-factors (NAD<sup>+</sup> and hexacyanoferrate(III)) required in the enzymatic system. The new device employed in this work allows the detection of 0.09 ppm thiram, a concentration below legal limits (Maximum Residue Limits 0.1–10 ppm).
format	article
author	David Ibáñez Daniel Izquierdo-Bote María Begoña González-García David Hernández-Santos Pablo Fanjul-Bolado
author_facet	David Ibáñez Daniel Izquierdo-Bote María Begoña González-García David Hernández-Santos Pablo Fanjul-Bolado
author_sort	David Ibáñez
title	Development of a New Screen-Printed Transducer for the Electrochemical Detection of Thiram
title_short	Development of a New Screen-Printed Transducer for the Electrochemical Detection of Thiram
title_full	Development of a New Screen-Printed Transducer for the Electrochemical Detection of Thiram
title_fullStr	Development of a New Screen-Printed Transducer for the Electrochemical Detection of Thiram
title_full_unstemmed	Development of a New Screen-Printed Transducer for the Electrochemical Detection of Thiram
title_sort	development of a new screen-printed transducer for the electrochemical detection of thiram
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/6144793019aa4ee69223102f6ccf4855
work_keys_str_mv	AT davidibanez developmentofanewscreenprintedtransducerfortheelectrochemicaldetectionofthiram AT danielizquierdobote developmentofanewscreenprintedtransducerfortheelectrochemicaldetectionofthiram AT mariabegonagonzalezgarcia developmentofanewscreenprintedtransducerfortheelectrochemicaldetectionofthiram AT davidhernandezsantos developmentofanewscreenprintedtransducerfortheelectrochemicaldetectionofthiram AT pablofanjulbolado developmentofanewscreenprintedtransducerfortheelectrochemicaldetectionofthiram
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Development of a New Screen-Printed Transducer for the Electrochemical Detection of Thiram

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