Use of immunomagnetic reduction for C-reactive protein assay in clinical samples

Chien-Hsi Chang,1 Zhi-Xian Lai,1 Hsiu-Li Lin,2 Che-Chuan Yang,3 Hsin-Hsien Chen,3,4 Shieh-Yueh Yang,5 Herng-Er Horng,3 Chin-Yih Hong,6 Hong-Chang Yang,4 Hsiu-Chen Lin1,71Department of Laboratory Medicine, Taipei Medical University Hospital, 2Department of Neurology, General Cathay Hospital, Sijhih B...

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Autores principales:	Chang CH, Lai ZX, Lin HL, Yang CC, Chen HH, Yang SY, Horng HE, Hong CY, Yang HC, Lin HC
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/6152b82839bc4d34ad6257e61952af29
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spelling	oai:doaj.org-article:6152b82839bc4d34ad6257e61952af292021-12-02T00:51:27ZUse of immunomagnetic reduction for C-reactive protein assay in clinical samples1176-91141178-2013https://doaj.org/article/6152b82839bc4d34ad6257e61952af292012-08-01T00:00:00Zhttp://www.dovepress.com/use-of-immunomagnetic-reduction-for-c-reactive-protein-assay-in-clinic-a10621https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Chien-Hsi Chang,1 Zhi-Xian Lai,1 Hsiu-Li Lin,2 Che-Chuan Yang,3 Hsin-Hsien Chen,3,4 Shieh-Yueh Yang,5 Herng-Er Horng,3 Chin-Yih Hong,6 Hong-Chang Yang,4 Hsiu-Chen Lin1,71Department of Laboratory Medicine, Taipei Medical University Hospital, 2Department of Neurology, General Cathay Hospital, Sijhih Branch, 3Institute of Electro-optical Science and Technology, National Taiwan Normal University, 4Department of Physics, National Taiwan University, 5MagQu Co, Ltd, Sindian District, New Taipei City, 6Department of Mechanical Engineering, Nan-Kai University of Technology, Nan-tau County, 7Department of Pediatrics, School of Medicine, College of Medicine, Taipei Medical University, Taipei, TaiwanBackground: Magnetic nanoparticles biofunctionalized with antibodies are able to recognize and bind to the corresponding antigens. In this work, anti-C-reactive protein (CRP) antibody was covalently conjugated onto the surface of magnetic nanoparticles to label CRP specifically in serum.Methods: The level of serum CRP was detected by immunomagnetic reduction (IMR) assay, which identifies the changes in the magnetic signal representing the level of interaction between antibody-conjugated magnetic nanoparticles and CRP proteins. To investigate the feasibility of IMR for clinical application, pure CRP solutions and 40 human serum samples were tested for IMR detection of CRP to characterize sensitivity, specificity, and interference.Results: In comparison with the immunoturbidimetry assay, the results of the IMR assay indicated higher sensitivity and had a high correlation with those of the current immunoturbidimetry assay.Conclusion: We have developed a novel and promising way to assay CRP in human serum using immunomagnetic reduction in clinical diagnosis.Keywords: magnetic nanoparticles, immunomagnetic reduction, C-reactive proteinChang CHLai ZXLin HLYang CCChen HHYang SYHorng HEHong CYYang HCLin HCDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 4335-4340 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Chang CH Lai ZX Lin HL Yang CC Chen HH Yang SY Horng HE Hong CY Yang HC Lin HC Use of immunomagnetic reduction for C-reactive protein assay in clinical samples
description	Chien-Hsi Chang,1 Zhi-Xian Lai,1 Hsiu-Li Lin,2 Che-Chuan Yang,3 Hsin-Hsien Chen,3,4 Shieh-Yueh Yang,5 Herng-Er Horng,3 Chin-Yih Hong,6 Hong-Chang Yang,4 Hsiu-Chen Lin1,71Department of Laboratory Medicine, Taipei Medical University Hospital, 2Department of Neurology, General Cathay Hospital, Sijhih Branch, 3Institute of Electro-optical Science and Technology, National Taiwan Normal University, 4Department of Physics, National Taiwan University, 5MagQu Co, Ltd, Sindian District, New Taipei City, 6Department of Mechanical Engineering, Nan-Kai University of Technology, Nan-tau County, 7Department of Pediatrics, School of Medicine, College of Medicine, Taipei Medical University, Taipei, TaiwanBackground: Magnetic nanoparticles biofunctionalized with antibodies are able to recognize and bind to the corresponding antigens. In this work, anti-C-reactive protein (CRP) antibody was covalently conjugated onto the surface of magnetic nanoparticles to label CRP specifically in serum.Methods: The level of serum CRP was detected by immunomagnetic reduction (IMR) assay, which identifies the changes in the magnetic signal representing the level of interaction between antibody-conjugated magnetic nanoparticles and CRP proteins. To investigate the feasibility of IMR for clinical application, pure CRP solutions and 40 human serum samples were tested for IMR detection of CRP to characterize sensitivity, specificity, and interference.Results: In comparison with the immunoturbidimetry assay, the results of the IMR assay indicated higher sensitivity and had a high correlation with those of the current immunoturbidimetry assay.Conclusion: We have developed a novel and promising way to assay CRP in human serum using immunomagnetic reduction in clinical diagnosis.Keywords: magnetic nanoparticles, immunomagnetic reduction, C-reactive protein
format	article
author	Chang CH Lai ZX Lin HL Yang CC Chen HH Yang SY Horng HE Hong CY Yang HC Lin HC
author_facet	Chang CH Lai ZX Lin HL Yang CC Chen HH Yang SY Horng HE Hong CY Yang HC Lin HC
author_sort	Chang CH
title	Use of immunomagnetic reduction for C-reactive protein assay in clinical samples
title_short	Use of immunomagnetic reduction for C-reactive protein assay in clinical samples
title_full	Use of immunomagnetic reduction for C-reactive protein assay in clinical samples
title_fullStr	Use of immunomagnetic reduction for C-reactive protein assay in clinical samples
title_full_unstemmed	Use of immunomagnetic reduction for C-reactive protein assay in clinical samples
title_sort	use of immunomagnetic reduction for c-reactive protein assay in clinical samples
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/6152b82839bc4d34ad6257e61952af29
work_keys_str_mv	AT changch useofimmunomagneticreductionforcreactiveproteinassayinclinicalsamples AT laizx useofimmunomagneticreductionforcreactiveproteinassayinclinicalsamples AT linhl useofimmunomagneticreductionforcreactiveproteinassayinclinicalsamples AT yangcc useofimmunomagneticreductionforcreactiveproteinassayinclinicalsamples AT chenhh useofimmunomagneticreductionforcreactiveproteinassayinclinicalsamples AT yangsy useofimmunomagneticreductionforcreactiveproteinassayinclinicalsamples AT hornghe useofimmunomagneticreductionforcreactiveproteinassayinclinicalsamples AT hongcy useofimmunomagneticreductionforcreactiveproteinassayinclinicalsamples AT yanghc useofimmunomagneticreductionforcreactiveproteinassayinclinicalsamples AT linhc useofimmunomagneticreductionforcreactiveproteinassayinclinicalsamples
_version_	1718403441617797120

Use of immunomagnetic reduction for C-reactive protein assay in clinical samples

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