Investigations concerning the impact of consumption of hot beverages on acute cytotoxic and genotoxic effects in oral mucosa cells

Abstract Consumption of very hot beverages and foods increases the incidence of oral and esophageal cancer but the mechanisms are not known and the critical temperature is not well defined. We realized a study with exfoliated cells from the oral cavity of individuals (n = 73) that live in an area in...

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Autores principales:	Benjamin Ernst, Tahereh Setayesh, Armen Nersesyan, Michael Kundi, Michael Fenech, Claudia Bolognesi, Miroslav Mišík, Masood Ghane, Seyed Fazlollah Mousavi, Siegfried Knasmüller
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6156ff9568de464eb317d33cc3846e09
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spelling	oai:doaj.org-article:6156ff9568de464eb317d33cc3846e092021-11-28T12:19:13ZInvestigations concerning the impact of consumption of hot beverages on acute cytotoxic and genotoxic effects in oral mucosa cells10.1038/s41598-021-01995-92045-2322https://doaj.org/article/6156ff9568de464eb317d33cc3846e092021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01995-9https://doaj.org/toc/2045-2322Abstract Consumption of very hot beverages and foods increases the incidence of oral and esophageal cancer but the mechanisms are not known and the critical temperature is not well defined. We realized a study with exfoliated cells from the oral cavity of individuals (n = 73) that live in an area in Iran which has the highest incidence of EC worldwide. Consumption of beverages at very high temperatures is a characteristic feature of this population. We analyzed biomarkers which are (i) indicative for genetic instability (micronuclei that are formed as a consequence of chromosomal damage, nuclear buds which are a consequence of gene amplifications and binucleated cells which reflect mitotic disturbances), (ii) markers that reflect cytotoxic effects (condensed chromatin, karyorrhectic, karyolitic and pyknotic cells), (iii) furthermore, we determined the number of basal cells which is indicative for the regenerative capacity of the buccal mucosa. The impact of the drinking temperature on the frequencies of these parameters was monitored with thermometers. We found no evidence for induction of genetic damage but an increase of the cytotoxic effects with the temperature was evident. This effect was paralleled by an increase of the cell division rate of the mucosa which was observed when the temperature exceeded 60 °C. Our findings indicate that cancer in the upper digestive tract in drinkers of very hot beverages is not caused by damage of the genetic material but by an increase of the cell division rate as a consequence of cytotoxic effects which take place at temperatures over 60 °C. It is known from earlier experiments with rodents that increased cell divisions lead to tumor promotion in the esophagus. Our findings provide a mechanistic explanation and indicate that increased cancer risks can be expected when the drinking temperature of beverages exceeds 60 °C.Benjamin ErnstTahereh SetayeshArmen NersesyanMichael KundiMichael FenechClaudia BolognesiMiroslav MišíkMasood GhaneSeyed Fazlollah MousaviSiegfried KnasmüllerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Benjamin Ernst Tahereh Setayesh Armen Nersesyan Michael Kundi Michael Fenech Claudia Bolognesi Miroslav Mišík Masood Ghane Seyed Fazlollah Mousavi Siegfried Knasmüller Investigations concerning the impact of consumption of hot beverages on acute cytotoxic and genotoxic effects in oral mucosa cells
description	Abstract Consumption of very hot beverages and foods increases the incidence of oral and esophageal cancer but the mechanisms are not known and the critical temperature is not well defined. We realized a study with exfoliated cells from the oral cavity of individuals (n = 73) that live in an area in Iran which has the highest incidence of EC worldwide. Consumption of beverages at very high temperatures is a characteristic feature of this population. We analyzed biomarkers which are (i) indicative for genetic instability (micronuclei that are formed as a consequence of chromosomal damage, nuclear buds which are a consequence of gene amplifications and binucleated cells which reflect mitotic disturbances), (ii) markers that reflect cytotoxic effects (condensed chromatin, karyorrhectic, karyolitic and pyknotic cells), (iii) furthermore, we determined the number of basal cells which is indicative for the regenerative capacity of the buccal mucosa. The impact of the drinking temperature on the frequencies of these parameters was monitored with thermometers. We found no evidence for induction of genetic damage but an increase of the cytotoxic effects with the temperature was evident. This effect was paralleled by an increase of the cell division rate of the mucosa which was observed when the temperature exceeded 60 °C. Our findings indicate that cancer in the upper digestive tract in drinkers of very hot beverages is not caused by damage of the genetic material but by an increase of the cell division rate as a consequence of cytotoxic effects which take place at temperatures over 60 °C. It is known from earlier experiments with rodents that increased cell divisions lead to tumor promotion in the esophagus. Our findings provide a mechanistic explanation and indicate that increased cancer risks can be expected when the drinking temperature of beverages exceeds 60 °C.
format	article
author	Benjamin Ernst Tahereh Setayesh Armen Nersesyan Michael Kundi Michael Fenech Claudia Bolognesi Miroslav Mišík Masood Ghane Seyed Fazlollah Mousavi Siegfried Knasmüller
author_facet	Benjamin Ernst Tahereh Setayesh Armen Nersesyan Michael Kundi Michael Fenech Claudia Bolognesi Miroslav Mišík Masood Ghane Seyed Fazlollah Mousavi Siegfried Knasmüller
author_sort	Benjamin Ernst
title	Investigations concerning the impact of consumption of hot beverages on acute cytotoxic and genotoxic effects in oral mucosa cells
title_short	Investigations concerning the impact of consumption of hot beverages on acute cytotoxic and genotoxic effects in oral mucosa cells
title_full	Investigations concerning the impact of consumption of hot beverages on acute cytotoxic and genotoxic effects in oral mucosa cells
title_fullStr	Investigations concerning the impact of consumption of hot beverages on acute cytotoxic and genotoxic effects in oral mucosa cells
title_full_unstemmed	Investigations concerning the impact of consumption of hot beverages on acute cytotoxic and genotoxic effects in oral mucosa cells
title_sort	investigations concerning the impact of consumption of hot beverages on acute cytotoxic and genotoxic effects in oral mucosa cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6156ff9568de464eb317d33cc3846e09
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Investigations concerning the impact of consumption of hot beverages on acute cytotoxic and genotoxic effects in oral mucosa cells

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