Transcriptomic analysis of granulosa cell populations proximal and distal to the germinal disc of chicken preovulatory follicles

Abstract Within the oocytes of chicken preovulatory follicles, the engulfed yolk constitutes 99% of the oocyte content, while the small germinal disc (GD) (which contains the nucleus and 99% ooplasm) occupies only less than 1%. Relative to the position of the GD, the single granulosa cell layer surr...

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Autores principales:	Guoqiang Zhu, Chao Fang, Chunheng Mo, Yajun Wang, Yan Huang, Juan Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/61574f06ac504e48a52430960ac07e3f
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spelling	oai:doaj.org-article:61574f06ac504e48a52430960ac07e3f2021-12-02T13:34:32ZTranscriptomic analysis of granulosa cell populations proximal and distal to the germinal disc of chicken preovulatory follicles10.1038/s41598-021-84140-w2045-2322https://doaj.org/article/61574f06ac504e48a52430960ac07e3f2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84140-whttps://doaj.org/toc/2045-2322Abstract Within the oocytes of chicken preovulatory follicles, the engulfed yolk constitutes 99% of the oocyte content, while the small germinal disc (GD) (which contains the nucleus and 99% ooplasm) occupies only less than 1%. Relative to the position of the GD, the single granulosa cell layer surrounding the oocyte can be sub-divided into two sub-populations: granulosa cells proximal (named Gp cells) and distal (Gd cells) to the GD. It was reported that Gp cells and Gd cells differ in their morphology, proliferative rate and steroidogenic capacity, however, the underlying mechanism controlling granulosa cell heterogeneity remains unclear. Here we analyzed the transcriptomes of Gd and Gp cells of preovulatory (F5 and F1) follicles in chicken ovaries. We found that: (1) genes associated with cell cycle and DNA replication (CDK1, CCNB3 etc.) have comparatively higher expression levels in Gp cells than in Gd cells, while genes associated with steroidogenesis (CYP51A1, DHCR24) are highly expressed in Gd cells, indicating that Gp cells are likely more mitotic and less steroidogenic than Gd cells; (2) genes associated with extracellular matrix remodeling, cell adhesion and sperm binding (ZP3, ZP2) are differentially expressed in Gp and Gd cells; (3) Furthermore, signaling molecules (WNT4/IHH) and receptors for NGF (NGFR), epidermal growth factor (EGFR), gonadotropins (FSHR/LHR) and prostaglandin (PTGER3) are abundantly but differentially expressed in Gp and Gd cells. Taken together, our data strongly supports the notion that Gp and Gd cells of preovulatory follicles differ in their proliferation rate, steroidogenic activity, ECM organization and sperm binding capacity, which are likely controlled by gonadotropins and local ovarian factors, such as GD-derived factors.Guoqiang ZhuChao FangChunheng MoYajun WangYan HuangJuan LiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Guoqiang Zhu Chao Fang Chunheng Mo Yajun Wang Yan Huang Juan Li Transcriptomic analysis of granulosa cell populations proximal and distal to the germinal disc of chicken preovulatory follicles
description	Abstract Within the oocytes of chicken preovulatory follicles, the engulfed yolk constitutes 99% of the oocyte content, while the small germinal disc (GD) (which contains the nucleus and 99% ooplasm) occupies only less than 1%. Relative to the position of the GD, the single granulosa cell layer surrounding the oocyte can be sub-divided into two sub-populations: granulosa cells proximal (named Gp cells) and distal (Gd cells) to the GD. It was reported that Gp cells and Gd cells differ in their morphology, proliferative rate and steroidogenic capacity, however, the underlying mechanism controlling granulosa cell heterogeneity remains unclear. Here we analyzed the transcriptomes of Gd and Gp cells of preovulatory (F5 and F1) follicles in chicken ovaries. We found that: (1) genes associated with cell cycle and DNA replication (CDK1, CCNB3 etc.) have comparatively higher expression levels in Gp cells than in Gd cells, while genes associated with steroidogenesis (CYP51A1, DHCR24) are highly expressed in Gd cells, indicating that Gp cells are likely more mitotic and less steroidogenic than Gd cells; (2) genes associated with extracellular matrix remodeling, cell adhesion and sperm binding (ZP3, ZP2) are differentially expressed in Gp and Gd cells; (3) Furthermore, signaling molecules (WNT4/IHH) and receptors for NGF (NGFR), epidermal growth factor (EGFR), gonadotropins (FSHR/LHR) and prostaglandin (PTGER3) are abundantly but differentially expressed in Gp and Gd cells. Taken together, our data strongly supports the notion that Gp and Gd cells of preovulatory follicles differ in their proliferation rate, steroidogenic activity, ECM organization and sperm binding capacity, which are likely controlled by gonadotropins and local ovarian factors, such as GD-derived factors.
format	article
author	Guoqiang Zhu Chao Fang Chunheng Mo Yajun Wang Yan Huang Juan Li
author_facet	Guoqiang Zhu Chao Fang Chunheng Mo Yajun Wang Yan Huang Juan Li
author_sort	Guoqiang Zhu
title	Transcriptomic analysis of granulosa cell populations proximal and distal to the germinal disc of chicken preovulatory follicles
title_short	Transcriptomic analysis of granulosa cell populations proximal and distal to the germinal disc of chicken preovulatory follicles
title_full	Transcriptomic analysis of granulosa cell populations proximal and distal to the germinal disc of chicken preovulatory follicles
title_fullStr	Transcriptomic analysis of granulosa cell populations proximal and distal to the germinal disc of chicken preovulatory follicles
title_full_unstemmed	Transcriptomic analysis of granulosa cell populations proximal and distal to the germinal disc of chicken preovulatory follicles
title_sort	transcriptomic analysis of granulosa cell populations proximal and distal to the germinal disc of chicken preovulatory follicles
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/61574f06ac504e48a52430960ac07e3f
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Transcriptomic analysis of granulosa cell populations proximal and distal to the germinal disc of chicken preovulatory follicles

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