Assessment of <italic toggle="yes">In Vitro</italic> and <italic toggle="yes">In Silico</italic> Protocols for Sequence-Based Characterization of the Human Vaginal Microbiome

Assessment of <italic toggle="yes">In Vitro</italic> and <italic toggle="yes">In Silico</italic> Protocols for Sequence-Based Characterization of the Human Vaginal Microbiome

ABSTRACT The vaginal microbiome has been connected to a wide range of health outcomes. This has led to a thriving research environment but also to the use of conflicting methodologies to study its microbial composition. Here, we systematically assessed best practices for the sequencing-based charact...

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Main Authors: Luisa W. Hugerth, Marcela Pereira, Yinghua Zha, Maike Seifert, Vilde Kaldhusdal, Fredrik Boulund, Maria C. Krog, Zahra Bashir, Marica Hamsten, Emma Fransson, Henriette Svarre Nielsen, Ina Schuppe-Koistinen, Lars Engstrand
Format: article
Language:EN
Published: American Society for Microbiology 2020
Subjects:
16S rRNA
PCR
amplicon
human microbiome
metagenomics
molecular methods
Microbiology
QR1-502
Online Access:https://doaj.org/article/615c12678b544adf99d3e8489166a079
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https://doaj.org/article/615c12678b544adf99d3e8489166a079

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