Preliminary functional analysis of the subgingival microbiota of cats with periodontitis and feline chronic gingivostomatitis

Abstract The subgingival microbial communities of domestic cats remain incompletely characterized and it is unknown whether their functional profiles are associated with disease. In this study, we used a shotgun metagenomic approach to explore the functional potential of subgingival microbial commun...

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Autores principales:	Marjory Xavier Rodrigues, Nadine Fiani, Rodrigo Carvalho Bicalho, Santiago Peralta
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/615c793fe82241b2aaa713d5c9ac6f42
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spelling	oai:doaj.org-article:615c793fe82241b2aaa713d5c9ac6f422021-12-02T17:04:35ZPreliminary functional analysis of the subgingival microbiota of cats with periodontitis and feline chronic gingivostomatitis10.1038/s41598-021-86466-x2045-2322https://doaj.org/article/615c793fe82241b2aaa713d5c9ac6f422021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86466-xhttps://doaj.org/toc/2045-2322Abstract The subgingival microbial communities of domestic cats remain incompletely characterized and it is unknown whether their functional profiles are associated with disease. In this study, we used a shotgun metagenomic approach to explore the functional potential of subgingival microbial communities in client-owned cats, comparing findings between periodontally healthy cats and cats with naturally occurring chronic periodontitis, aggressive periodontitis, and feline chronic gingivostomatitis. Subgingival samples were subjected to shotgun sequencing and the metagenomic datasets were analyzed using the MG-RAST metagenomic analysis server and STAMP v2.1.3 (Statistical Analysis of Metagenomic Profiles) software. The microbial composition was also described to better understand the predicted features of the communities. The Respiration category in the level 1 Subsystems database varied significantly among groups. In this category, the abundance of V-Type ATP-synthase and Biogenesis of cytochrome c oxidases were significantly enriched in the diseased and in the healthy groups, respectively. Both features have been previously described in periodontal studies in people and are in consonance with the microbial composition of feline subgingival sites. In addition, the narH (nitrate reductase) gene frequency, identified using the KEGG Orthology database, was significantly increased in the healthy group. The results of this study provide preliminary functional insights of the microbial communities associated with periodontitis in domestic cats and suggest that the ATP-synthase and nitrate-nitrite-NO pathways may represent appropriate targets for the treatment of this common disease.Marjory Xavier RodriguesNadine FianiRodrigo Carvalho BicalhoSantiago PeraltaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marjory Xavier Rodrigues Nadine Fiani Rodrigo Carvalho Bicalho Santiago Peralta Preliminary functional analysis of the subgingival microbiota of cats with periodontitis and feline chronic gingivostomatitis
description	Abstract The subgingival microbial communities of domestic cats remain incompletely characterized and it is unknown whether their functional profiles are associated with disease. In this study, we used a shotgun metagenomic approach to explore the functional potential of subgingival microbial communities in client-owned cats, comparing findings between periodontally healthy cats and cats with naturally occurring chronic periodontitis, aggressive periodontitis, and feline chronic gingivostomatitis. Subgingival samples were subjected to shotgun sequencing and the metagenomic datasets were analyzed using the MG-RAST metagenomic analysis server and STAMP v2.1.3 (Statistical Analysis of Metagenomic Profiles) software. The microbial composition was also described to better understand the predicted features of the communities. The Respiration category in the level 1 Subsystems database varied significantly among groups. In this category, the abundance of V-Type ATP-synthase and Biogenesis of cytochrome c oxidases were significantly enriched in the diseased and in the healthy groups, respectively. Both features have been previously described in periodontal studies in people and are in consonance with the microbial composition of feline subgingival sites. In addition, the narH (nitrate reductase) gene frequency, identified using the KEGG Orthology database, was significantly increased in the healthy group. The results of this study provide preliminary functional insights of the microbial communities associated with periodontitis in domestic cats and suggest that the ATP-synthase and nitrate-nitrite-NO pathways may represent appropriate targets for the treatment of this common disease.
format	article
author	Marjory Xavier Rodrigues Nadine Fiani Rodrigo Carvalho Bicalho Santiago Peralta
author_facet	Marjory Xavier Rodrigues Nadine Fiani Rodrigo Carvalho Bicalho Santiago Peralta
author_sort	Marjory Xavier Rodrigues
title	Preliminary functional analysis of the subgingival microbiota of cats with periodontitis and feline chronic gingivostomatitis
title_short	Preliminary functional analysis of the subgingival microbiota of cats with periodontitis and feline chronic gingivostomatitis
title_full	Preliminary functional analysis of the subgingival microbiota of cats with periodontitis and feline chronic gingivostomatitis
title_fullStr	Preliminary functional analysis of the subgingival microbiota of cats with periodontitis and feline chronic gingivostomatitis
title_full_unstemmed	Preliminary functional analysis of the subgingival microbiota of cats with periodontitis and feline chronic gingivostomatitis
title_sort	preliminary functional analysis of the subgingival microbiota of cats with periodontitis and feline chronic gingivostomatitis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/615c793fe82241b2aaa713d5c9ac6f42
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Preliminary functional analysis of the subgingival microbiota of cats with periodontitis and feline chronic gingivostomatitis

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