Proteomic Characterization of Plasma Rich in Growth Factors and Undiluted Autologous Serum

Over the last three decades, there has been special interest in developing drugs that mimic the characteristics of natural tears for use it in the treatment of several ocular surface disorders. Interestingly, the composition of blood plasma is very similar to tears. Therefore, different blood-derive...

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Autores principales:	Eduardo Anitua, Francisco Muruzabal, Ander Pino, Roberto Prado, Mikel Azkargorta, Felix Elortza, Jesús Merayo-Lloves
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	platelet-rich plasma PRP plasma rich in growth factors PRGF autologous serum AS Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/615edb7e9cd84b6fa2b937b110ead923
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id	oai:doaj.org-article:615edb7e9cd84b6fa2b937b110ead923
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spelling	oai:doaj.org-article:615edb7e9cd84b6fa2b937b110ead9232021-11-25T17:53:59ZProteomic Characterization of Plasma Rich in Growth Factors and Undiluted Autologous Serum10.3390/ijms2222121761422-00671661-6596https://doaj.org/article/615edb7e9cd84b6fa2b937b110ead9232021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12176https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Over the last three decades, there has been special interest in developing drugs that mimic the characteristics of natural tears for use it in the treatment of several ocular surface disorders. Interestingly, the composition of blood plasma is very similar to tears. Therefore, different blood-derived products like autologous serum (AS) and plasma rich in growth factors (PRGF) have been developed for the treatment of diverse ocular pathologies. However, scarce studies have been carried out to analyze the differences between both types of blood-derived products. In the present study, blood from three healthy donors was drawn and processed to obtain AS and PRGF eye drops. Then, human corneal stromal keratocytes (HK) were treated with PRGF or undiluted AS. Proteomic analysis was carried out to analyze and characterize the differential protein profiles between PRGF and AS, and the differentially expressed proteins in HK cells after PRGF and AS treatment. The results obtained in the present study show that undiluted AS induces the activation of different pathways related to an inflammatory, angiogenic, oxidative stress and scarring response in HK cells regarding PRGF. These results suggest that PRGF could be a better alternative than AS for the treatment of ocular surface disorders.Eduardo AnituaFrancisco MuruzabalAnder PinoRoberto PradoMikel AzkargortaFelix ElortzaJesús Merayo-LlovesMDPI AGarticleplatelet-rich plasmaPRPplasma rich in growth factorsPRGFautologous serumASBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12176, p 12176 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	platelet-rich plasma PRP plasma rich in growth factors PRGF autologous serum AS Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	platelet-rich plasma PRP plasma rich in growth factors PRGF autologous serum AS Biology (General) QH301-705.5 Chemistry QD1-999 Eduardo Anitua Francisco Muruzabal Ander Pino Roberto Prado Mikel Azkargorta Felix Elortza Jesús Merayo-Lloves Proteomic Characterization of Plasma Rich in Growth Factors and Undiluted Autologous Serum
description	Over the last three decades, there has been special interest in developing drugs that mimic the characteristics of natural tears for use it in the treatment of several ocular surface disorders. Interestingly, the composition of blood plasma is very similar to tears. Therefore, different blood-derived products like autologous serum (AS) and plasma rich in growth factors (PRGF) have been developed for the treatment of diverse ocular pathologies. However, scarce studies have been carried out to analyze the differences between both types of blood-derived products. In the present study, blood from three healthy donors was drawn and processed to obtain AS and PRGF eye drops. Then, human corneal stromal keratocytes (HK) were treated with PRGF or undiluted AS. Proteomic analysis was carried out to analyze and characterize the differential protein profiles between PRGF and AS, and the differentially expressed proteins in HK cells after PRGF and AS treatment. The results obtained in the present study show that undiluted AS induces the activation of different pathways related to an inflammatory, angiogenic, oxidative stress and scarring response in HK cells regarding PRGF. These results suggest that PRGF could be a better alternative than AS for the treatment of ocular surface disorders.
format	article
author	Eduardo Anitua Francisco Muruzabal Ander Pino Roberto Prado Mikel Azkargorta Felix Elortza Jesús Merayo-Lloves
author_facet	Eduardo Anitua Francisco Muruzabal Ander Pino Roberto Prado Mikel Azkargorta Felix Elortza Jesús Merayo-Lloves
author_sort	Eduardo Anitua
title	Proteomic Characterization of Plasma Rich in Growth Factors and Undiluted Autologous Serum
title_short	Proteomic Characterization of Plasma Rich in Growth Factors and Undiluted Autologous Serum
title_full	Proteomic Characterization of Plasma Rich in Growth Factors and Undiluted Autologous Serum
title_fullStr	Proteomic Characterization of Plasma Rich in Growth Factors and Undiluted Autologous Serum
title_full_unstemmed	Proteomic Characterization of Plasma Rich in Growth Factors and Undiluted Autologous Serum
title_sort	proteomic characterization of plasma rich in growth factors and undiluted autologous serum
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/615edb7e9cd84b6fa2b937b110ead923
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Proteomic Characterization of Plasma Rich in Growth Factors and Undiluted Autologous Serum

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