Phanta: a non-fluorescent photochromic acceptor for pcFRET.

We have developed an orange non-fluorescent photochromic protein (quantum yield, 0.003) we call Phanta that is useful as an acceptor in pcFRET applications. Phanta can be repeatedly inter-converted between the two absorbing states by alternate exposure to cyan and violet light. The absorption spectr...

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Autores principales:	Craig Don Paul, Csaba Kiss, Daouda A K Traore, Lan Gong, Matthew C J Wilce, Rodney J Devenish, Andrew Bradbury, Mark Prescott
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6164286db94546849ecbf39c58519ca3
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spelling	oai:doaj.org-article:6164286db94546849ecbf39c58519ca32021-11-18T08:53:14ZPhanta: a non-fluorescent photochromic acceptor for pcFRET.1932-620310.1371/journal.pone.0075835https://doaj.org/article/6164286db94546849ecbf39c58519ca32013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24098733/?tool=EBIhttps://doaj.org/toc/1932-6203We have developed an orange non-fluorescent photochromic protein (quantum yield, 0.003) we call Phanta that is useful as an acceptor in pcFRET applications. Phanta can be repeatedly inter-converted between the two absorbing states by alternate exposure to cyan and violet light. The absorption spectra of Phanta in one absorbing state shows excellent overlap with the emission spectra of a number of donor green fluorescent proteins including the commonly used EGFP. We show that the Phanta-EGFP FRET pair is suitable for monitoring the activation of caspase 3 in live cells using readily available instrumentation and a simple protocol that requires the acquisition of two donor emission images corresponding to Phanta in each of its photoswitched states. This the first report of a genetically encoded non-fluorescent acceptor for pcFRET.Craig Don PaulCsaba KissDaouda A K TraoreLan GongMatthew C J WilceRodney J DevenishAndrew BradburyMark PrescottPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 9, p e75835 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Craig Don Paul Csaba Kiss Daouda A K Traore Lan Gong Matthew C J Wilce Rodney J Devenish Andrew Bradbury Mark Prescott Phanta: a non-fluorescent photochromic acceptor for pcFRET.
description	We have developed an orange non-fluorescent photochromic protein (quantum yield, 0.003) we call Phanta that is useful as an acceptor in pcFRET applications. Phanta can be repeatedly inter-converted between the two absorbing states by alternate exposure to cyan and violet light. The absorption spectra of Phanta in one absorbing state shows excellent overlap with the emission spectra of a number of donor green fluorescent proteins including the commonly used EGFP. We show that the Phanta-EGFP FRET pair is suitable for monitoring the activation of caspase 3 in live cells using readily available instrumentation and a simple protocol that requires the acquisition of two donor emission images corresponding to Phanta in each of its photoswitched states. This the first report of a genetically encoded non-fluorescent acceptor for pcFRET.
format	article
author	Craig Don Paul Csaba Kiss Daouda A K Traore Lan Gong Matthew C J Wilce Rodney J Devenish Andrew Bradbury Mark Prescott
author_facet	Craig Don Paul Csaba Kiss Daouda A K Traore Lan Gong Matthew C J Wilce Rodney J Devenish Andrew Bradbury Mark Prescott
author_sort	Craig Don Paul
title	Phanta: a non-fluorescent photochromic acceptor for pcFRET.
title_short	Phanta: a non-fluorescent photochromic acceptor for pcFRET.
title_full	Phanta: a non-fluorescent photochromic acceptor for pcFRET.
title_fullStr	Phanta: a non-fluorescent photochromic acceptor for pcFRET.
title_full_unstemmed	Phanta: a non-fluorescent photochromic acceptor for pcFRET.
title_sort	phanta: a non-fluorescent photochromic acceptor for pcfret.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/6164286db94546849ecbf39c58519ca3
work_keys_str_mv	AT craigdonpaul phantaanonfluorescentphotochromicacceptorforpcfret AT csabakiss phantaanonfluorescentphotochromicacceptorforpcfret AT daoudaaktraore phantaanonfluorescentphotochromicacceptorforpcfret AT langong phantaanonfluorescentphotochromicacceptorforpcfret AT matthewcjwilce phantaanonfluorescentphotochromicacceptorforpcfret AT rodneyjdevenish phantaanonfluorescentphotochromicacceptorforpcfret AT andrewbradbury phantaanonfluorescentphotochromicacceptorforpcfret AT markprescott phantaanonfluorescentphotochromicacceptorforpcfret
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Phanta: a non-fluorescent photochromic acceptor for pcFRET.

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