Brainstem respiratory oscillators develop independently of neuronal migration defects in the Wnt/PCP mouse mutant looptail.

The proper development and maturation of neuronal circuits require precise migration of component neurons from their birthplace (germinal zone) to their final positions. Little is known about the effects of aberrant neuronal position on the functioning of organized neuronal groups, especially in mam...

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Autores principales:	Muriel Thoby-Brisson, Julien Bouvier, Derrick M Glasco, Michelle E Stewart, Charlotte Dean, Jennifer N Murdoch, Jean Champagnat, Gilles Fortin, Anand Chandrasekhar
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/616b6ba8dfe244c9ab9ef7793ca49140
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spelling	oai:doaj.org-article:616b6ba8dfe244c9ab9ef7793ca491402021-11-18T07:27:40ZBrainstem respiratory oscillators develop independently of neuronal migration defects in the Wnt/PCP mouse mutant looptail.1932-620310.1371/journal.pone.0031140https://doaj.org/article/616b6ba8dfe244c9ab9ef7793ca491402012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22363567/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The proper development and maturation of neuronal circuits require precise migration of component neurons from their birthplace (germinal zone) to their final positions. Little is known about the effects of aberrant neuronal position on the functioning of organized neuronal groups, especially in mammals. Here, we investigated the formation and properties of brainstem respiratory neurons in looptail (Lp) mutant mice in which facial motor neurons closely apposed to some respiratory neurons fail to migrate due to loss of function of the Wnt/Planar Cell Polarity (PCP) protein Vangl2. Using calcium imaging and immunostaining on embryonic hindbrain preparations, we found that respiratory neurons constituting the embryonic parafacial oscillator (e-pF) settled at the ventral surface of the medulla in Vangl2(Lp/+) and Vangl2(Lp/Lp) embryos despite the failure of tangential migration of its normally adjacent facial motor nucleus. Anatomically, the e-pF neurons were displaced medially in Lp/+ embryos and rostro-medially Lp/Lp embryos. Pharmacological treatments showed that the e-pF oscillator exhibited characteristic network properties in both Lp/+ and Lp/Lp embryos. Furthermore, using hindbrain slices, we found that the other respiratory oscillator, the preBötzinger complex, was also anatomically and functionally established in Lp mutants. Importantly, the displaced e-pF oscillator established functional connections with the preBötC oscillator in Lp/+ mutants. Our data highlight the robustness of the developmental processes that assemble the neuronal networks mediating an essential physiological function.Muriel Thoby-BrissonJulien BouvierDerrick M GlascoMichelle E StewartCharlotte DeanJennifer N MurdochJean ChampagnatGilles FortinAnand ChandrasekharPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 2, p e31140 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Muriel Thoby-Brisson Julien Bouvier Derrick M Glasco Michelle E Stewart Charlotte Dean Jennifer N Murdoch Jean Champagnat Gilles Fortin Anand Chandrasekhar Brainstem respiratory oscillators develop independently of neuronal migration defects in the Wnt/PCP mouse mutant looptail.
description	The proper development and maturation of neuronal circuits require precise migration of component neurons from their birthplace (germinal zone) to their final positions. Little is known about the effects of aberrant neuronal position on the functioning of organized neuronal groups, especially in mammals. Here, we investigated the formation and properties of brainstem respiratory neurons in looptail (Lp) mutant mice in which facial motor neurons closely apposed to some respiratory neurons fail to migrate due to loss of function of the Wnt/Planar Cell Polarity (PCP) protein Vangl2. Using calcium imaging and immunostaining on embryonic hindbrain preparations, we found that respiratory neurons constituting the embryonic parafacial oscillator (e-pF) settled at the ventral surface of the medulla in Vangl2(Lp/+) and Vangl2(Lp/Lp) embryos despite the failure of tangential migration of its normally adjacent facial motor nucleus. Anatomically, the e-pF neurons were displaced medially in Lp/+ embryos and rostro-medially Lp/Lp embryos. Pharmacological treatments showed that the e-pF oscillator exhibited characteristic network properties in both Lp/+ and Lp/Lp embryos. Furthermore, using hindbrain slices, we found that the other respiratory oscillator, the preBötzinger complex, was also anatomically and functionally established in Lp mutants. Importantly, the displaced e-pF oscillator established functional connections with the preBötC oscillator in Lp/+ mutants. Our data highlight the robustness of the developmental processes that assemble the neuronal networks mediating an essential physiological function.
format	article
author	Muriel Thoby-Brisson Julien Bouvier Derrick M Glasco Michelle E Stewart Charlotte Dean Jennifer N Murdoch Jean Champagnat Gilles Fortin Anand Chandrasekhar
author_facet	Muriel Thoby-Brisson Julien Bouvier Derrick M Glasco Michelle E Stewart Charlotte Dean Jennifer N Murdoch Jean Champagnat Gilles Fortin Anand Chandrasekhar
author_sort	Muriel Thoby-Brisson
title	Brainstem respiratory oscillators develop independently of neuronal migration defects in the Wnt/PCP mouse mutant looptail.
title_short	Brainstem respiratory oscillators develop independently of neuronal migration defects in the Wnt/PCP mouse mutant looptail.
title_full	Brainstem respiratory oscillators develop independently of neuronal migration defects in the Wnt/PCP mouse mutant looptail.
title_fullStr	Brainstem respiratory oscillators develop independently of neuronal migration defects in the Wnt/PCP mouse mutant looptail.
title_full_unstemmed	Brainstem respiratory oscillators develop independently of neuronal migration defects in the Wnt/PCP mouse mutant looptail.
title_sort	brainstem respiratory oscillators develop independently of neuronal migration defects in the wnt/pcp mouse mutant looptail.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/616b6ba8dfe244c9ab9ef7793ca49140
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Brainstem respiratory oscillators develop independently of neuronal migration defects in the Wnt/PCP mouse mutant looptail.

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