Development of a genetically encodable FRET system using fluorescent RNA aptamers

FRET has been used to study protein conformational changes but has never been applied to RNA aptamers. Here the authors develop a genetically encodable RNA aptamer-based FRET system on single-stranded RNA origami scaffolds, and demonstrate it can be used to study RNA conformational changes.

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Detalles Bibliográficos
Autores principales: Mette D. E. Jepsen, Steffen M. Sparvath, Thorbjørn B. Nielsen, Ane H. Langvad, Guido Grossi, Kurt V. Gothelf, Ebbe S. Andersen
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2018
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Acceso en línea:https://doaj.org/article/616f157ee0b5469fa10d778ba09eeffb
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Sumario:FRET has been used to study protein conformational changes but has never been applied to RNA aptamers. Here the authors develop a genetically encodable RNA aptamer-based FRET system on single-stranded RNA origami scaffolds, and demonstrate it can be used to study RNA conformational changes.