Development of a genetically encodable FRET system using fluorescent RNA aptamers

FRET has been used to study protein conformational changes but has never been applied to RNA aptamers. Here the authors develop a genetically encodable RNA aptamer-based FRET system on single-stranded RNA origami scaffolds, and demonstrate it can be used to study RNA conformational changes.

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Bibliographic Details
Main Authors:	Mette D. E. Jepsen, Steffen M. Sparvath, Thorbjørn B. Nielsen, Ane H. Langvad, Guido Grossi, Kurt V. Gothelf, Ebbe S. Andersen
Format:	article
Language:	EN
Published:	Nature Portfolio 2018
Subjects:	Science Q
Online Access:	https://doaj.org/article/616f157ee0b5469fa10d778ba09eeffb
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spelling	oai:doaj.org-article:616f157ee0b5469fa10d778ba09eeffb2021-12-02T15:34:50ZDevelopment of a genetically encodable FRET system using fluorescent RNA aptamers10.1038/s41467-017-02435-x2041-1723https://doaj.org/article/616f157ee0b5469fa10d778ba09eeffb2018-01-01T00:00:00Zhttps://doi.org/10.1038/s41467-017-02435-xhttps://doaj.org/toc/2041-1723FRET has been used to study protein conformational changes but has never been applied to RNA aptamers. Here the authors develop a genetically encodable RNA aptamer-based FRET system on single-stranded RNA origami scaffolds, and demonstrate it can be used to study RNA conformational changes.Mette D. E. JepsenSteffen M. SparvathThorbjørn B. NielsenAne H. LangvadGuido GrossiKurt V. GothelfEbbe S. AndersenNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Mette D. E. Jepsen Steffen M. Sparvath Thorbjørn B. Nielsen Ane H. Langvad Guido Grossi Kurt V. Gothelf Ebbe S. Andersen Development of a genetically encodable FRET system using fluorescent RNA aptamers
description	FRET has been used to study protein conformational changes but has never been applied to RNA aptamers. Here the authors develop a genetically encodable RNA aptamer-based FRET system on single-stranded RNA origami scaffolds, and demonstrate it can be used to study RNA conformational changes.
format	article
author	Mette D. E. Jepsen Steffen M. Sparvath Thorbjørn B. Nielsen Ane H. Langvad Guido Grossi Kurt V. Gothelf Ebbe S. Andersen
author_facet	Mette D. E. Jepsen Steffen M. Sparvath Thorbjørn B. Nielsen Ane H. Langvad Guido Grossi Kurt V. Gothelf Ebbe S. Andersen
author_sort	Mette D. E. Jepsen
title	Development of a genetically encodable FRET system using fluorescent RNA aptamers
title_short	Development of a genetically encodable FRET system using fluorescent RNA aptamers
title_full	Development of a genetically encodable FRET system using fluorescent RNA aptamers
title_fullStr	Development of a genetically encodable FRET system using fluorescent RNA aptamers
title_full_unstemmed	Development of a genetically encodable FRET system using fluorescent RNA aptamers
title_sort	development of a genetically encodable fret system using fluorescent rna aptamers
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/616f157ee0b5469fa10d778ba09eeffb
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Development of a genetically encodable FRET system using fluorescent RNA aptamers

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