Development of a genetically encodable FRET system using fluorescent RNA aptamers

FRET has been used to study protein conformational changes but has never been applied to RNA aptamers. Here the authors develop a genetically encodable RNA aptamer-based FRET system on single-stranded RNA origami scaffolds, and demonstrate it can be used to study RNA conformational changes.

Guardado en:

Detalles Bibliográficos
Autores principales:	Mette D. E. Jepsen, Steffen M. Sparvath, Thorbjørn B. Nielsen, Ane H. Langvad, Guido Grossi, Kurt V. Gothelf, Ebbe S. Andersen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/616f157ee0b5469fa10d778ba09eeffb
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:616f157ee0b5469fa10d778ba09eeffb
record_format	dspace
spelling	oai:doaj.org-article:616f157ee0b5469fa10d778ba09eeffb2021-12-02T15:34:50ZDevelopment of a genetically encodable FRET system using fluorescent RNA aptamers10.1038/s41467-017-02435-x2041-1723https://doaj.org/article/616f157ee0b5469fa10d778ba09eeffb2018-01-01T00:00:00Zhttps://doi.org/10.1038/s41467-017-02435-xhttps://doaj.org/toc/2041-1723FRET has been used to study protein conformational changes but has never been applied to RNA aptamers. Here the authors develop a genetically encodable RNA aptamer-based FRET system on single-stranded RNA origami scaffolds, and demonstrate it can be used to study RNA conformational changes.Mette D. E. JepsenSteffen M. SparvathThorbjørn B. NielsenAne H. LangvadGuido GrossiKurt V. GothelfEbbe S. AndersenNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Mette D. E. Jepsen Steffen M. Sparvath Thorbjørn B. Nielsen Ane H. Langvad Guido Grossi Kurt V. Gothelf Ebbe S. Andersen Development of a genetically encodable FRET system using fluorescent RNA aptamers
description	FRET has been used to study protein conformational changes but has never been applied to RNA aptamers. Here the authors develop a genetically encodable RNA aptamer-based FRET system on single-stranded RNA origami scaffolds, and demonstrate it can be used to study RNA conformational changes.
format	article
author	Mette D. E. Jepsen Steffen M. Sparvath Thorbjørn B. Nielsen Ane H. Langvad Guido Grossi Kurt V. Gothelf Ebbe S. Andersen
author_facet	Mette D. E. Jepsen Steffen M. Sparvath Thorbjørn B. Nielsen Ane H. Langvad Guido Grossi Kurt V. Gothelf Ebbe S. Andersen
author_sort	Mette D. E. Jepsen
title	Development of a genetically encodable FRET system using fluorescent RNA aptamers
title_short	Development of a genetically encodable FRET system using fluorescent RNA aptamers
title_full	Development of a genetically encodable FRET system using fluorescent RNA aptamers
title_fullStr	Development of a genetically encodable FRET system using fluorescent RNA aptamers
title_full_unstemmed	Development of a genetically encodable FRET system using fluorescent RNA aptamers
title_sort	development of a genetically encodable fret system using fluorescent rna aptamers
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/616f157ee0b5469fa10d778ba09eeffb
work_keys_str_mv	AT mettedejepsen developmentofageneticallyencodablefretsystemusingfluorescentrnaaptamers AT steffenmsparvath developmentofageneticallyencodablefretsystemusingfluorescentrnaaptamers AT thorbjørnbnielsen developmentofageneticallyencodablefretsystemusingfluorescentrnaaptamers AT anehlangvad developmentofageneticallyencodablefretsystemusingfluorescentrnaaptamers AT guidogrossi developmentofageneticallyencodablefretsystemusingfluorescentrnaaptamers AT kurtvgothelf developmentofageneticallyencodablefretsystemusingfluorescentrnaaptamers AT ebbesandersen developmentofageneticallyencodablefretsystemusingfluorescentrnaaptamers
_version_	1718386721958133760

Development of a genetically encodable FRET system using fluorescent RNA aptamers

Ejemplares similares