Wza gene knockout decreases Acinetobacter baumannii virulence and affects Wzy-dependent capsular polysaccharide synthesis

To investigate the virulence of capsular polysaccharide export protein (Wza) in carbapenem-resistant Acinetobacter baumannii and its effect on capsule formation. wza gene knockout and complementation strains were constructed, and changes in bacterial virulence were observed using in vitro adhesion,...

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Autores principales:	Tianshui Niu, Lihua Guo, Qixia Luo, Kai Zhou, Wei Yu, Yunbo Chen, Chen Huang, Yonghong Xiao
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2020
Materias:	acinetobacter baumannii virulence wza gene knockout gene complementation Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/6172f1d371a649688eaed475d6c10cde
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spelling	oai:doaj.org-article:6172f1d371a649688eaed475d6c10cde2021-11-17T14:21:57ZWza gene knockout decreases Acinetobacter baumannii virulence and affects Wzy-dependent capsular polysaccharide synthesis2150-55942150-560810.1080/21505594.2019.1700659https://doaj.org/article/6172f1d371a649688eaed475d6c10cde2020-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2019.1700659https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608To investigate the virulence of capsular polysaccharide export protein (Wza) in carbapenem-resistant Acinetobacter baumannii and its effect on capsule formation. wza gene knockout and complementation strains were constructed, and changes in bacterial virulence were observed using in vitro adhesion, antiserum complement killing, anti-oxidation experiments, and infections in Galleria mellonella and mice. The effect of wza knockout on the genes wzb and wzc and wzi were assessed by RT-PCR. We successfully constructed wza knockout and complementation strains. Compared with wild-type (WT) strains, wza knockout strains displayed lower adhesion to A549 cells (p = 0.044), lower antiserum complement killing ability (p = 0.001), and lower mortality of G. mellonella (p = 0.010) and mice (p = 0.033). Expression levels of wzb, wzc and wzi were decreased in wza knockout strains. The antioxidant capacity of Wza knockout bacteria was only slightly decreased. Complementation of the wza gene returned the adhesion ability, antiserum complement killing ability, and mortality of G. mellonella and mice to WT levels. Expression of wzb, wzc and wzi was also returned to WT levels following wza complementation. The results clearly demonstrate that Wza is toxic. Wza affects the expression of other proteins of the Wzy capsule polysaccharide synthesis pathway, which affects the assembly, export, and extracellular fixation of capsular polysaccharide, resulting in synergistic effects that decrease bacterial virulence.Tianshui NiuLihua GuoQixia LuoKai ZhouWei YuYunbo ChenChen HuangYonghong XiaoTaylor & Francis Grouparticleacinetobacter baumanniivirulencewzagene knockoutgene complementationInfectious and parasitic diseasesRC109-216ENVirulence, Vol 11, Iss 1, Pp 1-13 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	acinetobacter baumannii virulence wza gene knockout gene complementation Infectious and parasitic diseases RC109-216
spellingShingle	acinetobacter baumannii virulence wza gene knockout gene complementation Infectious and parasitic diseases RC109-216 Tianshui Niu Lihua Guo Qixia Luo Kai Zhou Wei Yu Yunbo Chen Chen Huang Yonghong Xiao Wza gene knockout decreases Acinetobacter baumannii virulence and affects Wzy-dependent capsular polysaccharide synthesis
description	To investigate the virulence of capsular polysaccharide export protein (Wza) in carbapenem-resistant Acinetobacter baumannii and its effect on capsule formation. wza gene knockout and complementation strains were constructed, and changes in bacterial virulence were observed using in vitro adhesion, antiserum complement killing, anti-oxidation experiments, and infections in Galleria mellonella and mice. The effect of wza knockout on the genes wzb and wzc and wzi were assessed by RT-PCR. We successfully constructed wza knockout and complementation strains. Compared with wild-type (WT) strains, wza knockout strains displayed lower adhesion to A549 cells (p = 0.044), lower antiserum complement killing ability (p = 0.001), and lower mortality of G. mellonella (p = 0.010) and mice (p = 0.033). Expression levels of wzb, wzc and wzi were decreased in wza knockout strains. The antioxidant capacity of Wza knockout bacteria was only slightly decreased. Complementation of the wza gene returned the adhesion ability, antiserum complement killing ability, and mortality of G. mellonella and mice to WT levels. Expression of wzb, wzc and wzi was also returned to WT levels following wza complementation. The results clearly demonstrate that Wza is toxic. Wza affects the expression of other proteins of the Wzy capsule polysaccharide synthesis pathway, which affects the assembly, export, and extracellular fixation of capsular polysaccharide, resulting in synergistic effects that decrease bacterial virulence.
format	article
author	Tianshui Niu Lihua Guo Qixia Luo Kai Zhou Wei Yu Yunbo Chen Chen Huang Yonghong Xiao
author_facet	Tianshui Niu Lihua Guo Qixia Luo Kai Zhou Wei Yu Yunbo Chen Chen Huang Yonghong Xiao
author_sort	Tianshui Niu
title	Wza gene knockout decreases Acinetobacter baumannii virulence and affects Wzy-dependent capsular polysaccharide synthesis
title_short	Wza gene knockout decreases Acinetobacter baumannii virulence and affects Wzy-dependent capsular polysaccharide synthesis
title_full	Wza gene knockout decreases Acinetobacter baumannii virulence and affects Wzy-dependent capsular polysaccharide synthesis
title_fullStr	Wza gene knockout decreases Acinetobacter baumannii virulence and affects Wzy-dependent capsular polysaccharide synthesis
title_full_unstemmed	Wza gene knockout decreases Acinetobacter baumannii virulence and affects Wzy-dependent capsular polysaccharide synthesis
title_sort	wza gene knockout decreases acinetobacter baumannii virulence and affects wzy-dependent capsular polysaccharide synthesis
publisher	Taylor & Francis Group
publishDate	2020
url	https://doaj.org/article/6172f1d371a649688eaed475d6c10cde
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Wza gene knockout decreases Acinetobacter baumannii virulence and affects Wzy-dependent capsular polysaccharide synthesis

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