Changes in Blood Metabolites, Intestinal Microbiota Composition and Gene Expression of 95 Weeks Old Laying Hens Differing in Egg Production and Egg Breaking Strength

Herein, we investigated to what extent molecular phenotypes of the systemic level (blood) and local (intestine) are associated with the performance of laying hens at 95 weeks of age. After the trial had run for 95 weeks, two performance groups were generated, i.e., egg production (PROD) and egg brea...

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Autores principales:	Dirkjan Schokker, Jeroen Visscher, Henri Woelders
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	laying hens vitality (blood) metabolites intestinal gene expression intestinal microbiota Veterinary medicine SF600-1100 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/6175f5ba84ec4ef8842853becd4bbcc8
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spelling	oai:doaj.org-article:6175f5ba84ec4ef8842853becd4bbcc82021-11-25T16:13:34ZChanges in Blood Metabolites, Intestinal Microbiota Composition and Gene Expression of 95 Weeks Old Laying Hens Differing in Egg Production and Egg Breaking Strength10.3390/ani111130122076-2615https://doaj.org/article/6175f5ba84ec4ef8842853becd4bbcc82021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2615/11/11/3012https://doaj.org/toc/2076-2615Herein, we investigated to what extent molecular phenotypes of the systemic level (blood) and local (intestine) are associated with the performance of laying hens at 95 weeks of age. After the trial had run for 95 weeks, two performance groups were generated, i.e., egg production (PROD) and egg breaking strength (BS). A subset of 21 cages, 116 hens, was measured to indicate the metabolism and disease status. Additionally, a focus group (four cages) was made to perform molecular phenotyping in the intestine. A notifiable observation made during the post-mortem dissection was that approximately 12% of the birds at 95 weeks had developed certain aberrations and/or impairments (denoted as organ morbidity). At the systemic level, we observed five metabolites (<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi mathvariant="sans-serif">γ</mi></semantics></math></inline-formula>GT, triglycerides, HDL, glucose, and cholesterol) significantly associated to organ morbidity, and only two metabolites (urea and aspartate aminotransferase) to the performance phenotypes. At the local level, when comparing high PROD vs. low PROD, we observed differentially expressed genes involved in cell cycle processes and the extracellular matrix. When comparing high BS vs. low BS differentially, expressed genes were observed mainly involved in immune and cell cycle-related processes. This knowledge is crucial for developing novel strategies of keeping laying hens vital.Dirkjan SchokkerJeroen VisscherHenri WoeldersMDPI AGarticlelaying hensvitality(blood) metabolitesintestinal gene expressionintestinal microbiotaVeterinary medicineSF600-1100ZoologyQL1-991ENAnimals, Vol 11, Iss 3012, p 3012 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	laying hens vitality (blood) metabolites intestinal gene expression intestinal microbiota Veterinary medicine SF600-1100 Zoology QL1-991
spellingShingle	laying hens vitality (blood) metabolites intestinal gene expression intestinal microbiota Veterinary medicine SF600-1100 Zoology QL1-991 Dirkjan Schokker Jeroen Visscher Henri Woelders Changes in Blood Metabolites, Intestinal Microbiota Composition and Gene Expression of 95 Weeks Old Laying Hens Differing in Egg Production and Egg Breaking Strength
description	Herein, we investigated to what extent molecular phenotypes of the systemic level (blood) and local (intestine) are associated with the performance of laying hens at 95 weeks of age. After the trial had run for 95 weeks, two performance groups were generated, i.e., egg production (PROD) and egg breaking strength (BS). A subset of 21 cages, 116 hens, was measured to indicate the metabolism and disease status. Additionally, a focus group (four cages) was made to perform molecular phenotyping in the intestine. A notifiable observation made during the post-mortem dissection was that approximately 12% of the birds at 95 weeks had developed certain aberrations and/or impairments (denoted as organ morbidity). At the systemic level, we observed five metabolites (<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi mathvariant="sans-serif">γ</mi></semantics></math></inline-formula>GT, triglycerides, HDL, glucose, and cholesterol) significantly associated to organ morbidity, and only two metabolites (urea and aspartate aminotransferase) to the performance phenotypes. At the local level, when comparing high PROD vs. low PROD, we observed differentially expressed genes involved in cell cycle processes and the extracellular matrix. When comparing high BS vs. low BS differentially, expressed genes were observed mainly involved in immune and cell cycle-related processes. This knowledge is crucial for developing novel strategies of keeping laying hens vital.
format	article
author	Dirkjan Schokker Jeroen Visscher Henri Woelders
author_facet	Dirkjan Schokker Jeroen Visscher Henri Woelders
author_sort	Dirkjan Schokker
title	Changes in Blood Metabolites, Intestinal Microbiota Composition and Gene Expression of 95 Weeks Old Laying Hens Differing in Egg Production and Egg Breaking Strength
title_short	Changes in Blood Metabolites, Intestinal Microbiota Composition and Gene Expression of 95 Weeks Old Laying Hens Differing in Egg Production and Egg Breaking Strength
title_full	Changes in Blood Metabolites, Intestinal Microbiota Composition and Gene Expression of 95 Weeks Old Laying Hens Differing in Egg Production and Egg Breaking Strength
title_fullStr	Changes in Blood Metabolites, Intestinal Microbiota Composition and Gene Expression of 95 Weeks Old Laying Hens Differing in Egg Production and Egg Breaking Strength
title_full_unstemmed	Changes in Blood Metabolites, Intestinal Microbiota Composition and Gene Expression of 95 Weeks Old Laying Hens Differing in Egg Production and Egg Breaking Strength
title_sort	changes in blood metabolites, intestinal microbiota composition and gene expression of 95 weeks old laying hens differing in egg production and egg breaking strength
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/6175f5ba84ec4ef8842853becd4bbcc8
work_keys_str_mv	AT dirkjanschokker changesinbloodmetabolitesintestinalmicrobiotacompositionandgeneexpressionof95weeksoldlayinghensdifferingineggproductionandeggbreakingstrength AT jeroenvisscher changesinbloodmetabolitesintestinalmicrobiotacompositionandgeneexpressionof95weeksoldlayinghensdifferingineggproductionandeggbreakingstrength AT henriwoelders changesinbloodmetabolitesintestinalmicrobiotacompositionandgeneexpressionof95weeksoldlayinghensdifferingineggproductionandeggbreakingstrength
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Changes in Blood Metabolites, Intestinal Microbiota Composition and Gene Expression of 95 Weeks Old Laying Hens Differing in Egg Production and Egg Breaking Strength

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