Bacterial profiling of Haemonchus contortus gut microbiome infecting Dohne Merino sheep in South Africa

Abstract A metagenomic approach was used to study the gut microbiome of Haemonchus contortus field strains and that of its predilection site, the abomasum of Dohne Merino sheep. The abomasum contents and H. contortus were collected from 10 naturally infected Dohne Merino sheep. The H. contortus spec...

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Autores principales:	T. Mafuna, P. Soma, A. M. Tsotetsi-Khambule, C. A. Hefer, F. C. Muchadeyi, O. M. M. Thekisoe, R. E. Pierneef
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/617967604052430babe4d33e820a001d
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spelling	oai:doaj.org-article:617967604052430babe4d33e820a001d2021-12-02T13:18:02ZBacterial profiling of Haemonchus contortus gut microbiome infecting Dohne Merino sheep in South Africa10.1038/s41598-021-85282-72045-2322https://doaj.org/article/617967604052430babe4d33e820a001d2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85282-7https://doaj.org/toc/2045-2322Abstract A metagenomic approach was used to study the gut microbiome of Haemonchus contortus field strains and that of its predilection site, the abomasum of Dohne Merino sheep. The abomasum contents and H. contortus were collected from 10 naturally infected Dohne Merino sheep. The H. contortus specimens were classified and sexually differentiated using morphometric characters and was further confirmed through molecular identification. We investigated differences and similarities between the bacterial composition of the adult male and female H. contortus gut microbiomes, which were both dominated by bacteria from the Escherichia, Shigella, Vibrio and Halomonas genera. Major abundance variations were identified between the shared adult male and female H. contortus microbiomes. The results also revealed that Succiniclasticum, Rikenellaceae RC9 gut group and Candidatus Saccharimonas were the predominant genera in the Dohne Merino abomasum. This study provides insight into the highly diverse bacterial composition of the H. contortus gut microbiome and the Dohne Merino abomasum which needs to be studied further to explore the complex interactions of different gastrointestinal nematode microbiomes with the host.T. MafunaP. SomaA. M. Tsotetsi-KhambuleC. A. HeferF. C. MuchadeyiO. M. M. ThekisoeR. E. PierneefNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q T. Mafuna P. Soma A. M. Tsotetsi-Khambule C. A. Hefer F. C. Muchadeyi O. M. M. Thekisoe R. E. Pierneef Bacterial profiling of Haemonchus contortus gut microbiome infecting Dohne Merino sheep in South Africa
description	Abstract A metagenomic approach was used to study the gut microbiome of Haemonchus contortus field strains and that of its predilection site, the abomasum of Dohne Merino sheep. The abomasum contents and H. contortus were collected from 10 naturally infected Dohne Merino sheep. The H. contortus specimens were classified and sexually differentiated using morphometric characters and was further confirmed through molecular identification. We investigated differences and similarities between the bacterial composition of the adult male and female H. contortus gut microbiomes, which were both dominated by bacteria from the Escherichia, Shigella, Vibrio and Halomonas genera. Major abundance variations were identified between the shared adult male and female H. contortus microbiomes. The results also revealed that Succiniclasticum, Rikenellaceae RC9 gut group and Candidatus Saccharimonas were the predominant genera in the Dohne Merino abomasum. This study provides insight into the highly diverse bacterial composition of the H. contortus gut microbiome and the Dohne Merino abomasum which needs to be studied further to explore the complex interactions of different gastrointestinal nematode microbiomes with the host.
format	article
author	T. Mafuna P. Soma A. M. Tsotetsi-Khambule C. A. Hefer F. C. Muchadeyi O. M. M. Thekisoe R. E. Pierneef
author_facet	T. Mafuna P. Soma A. M. Tsotetsi-Khambule C. A. Hefer F. C. Muchadeyi O. M. M. Thekisoe R. E. Pierneef
author_sort	T. Mafuna
title	Bacterial profiling of Haemonchus contortus gut microbiome infecting Dohne Merino sheep in South Africa
title_short	Bacterial profiling of Haemonchus contortus gut microbiome infecting Dohne Merino sheep in South Africa
title_full	Bacterial profiling of Haemonchus contortus gut microbiome infecting Dohne Merino sheep in South Africa
title_fullStr	Bacterial profiling of Haemonchus contortus gut microbiome infecting Dohne Merino sheep in South Africa
title_full_unstemmed	Bacterial profiling of Haemonchus contortus gut microbiome infecting Dohne Merino sheep in South Africa
title_sort	bacterial profiling of haemonchus contortus gut microbiome infecting dohne merino sheep in south africa
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/617967604052430babe4d33e820a001d
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Bacterial profiling of Haemonchus contortus gut microbiome infecting Dohne Merino sheep in South Africa

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