Construction of Genomic Library and Screening of Edwardsiella tarda Immunogenic Proteins for Their Protective Efficacy Against Edwardsiellosis

Edwardsiella tarda is a severe aquaculture pathogen that can infect many hosts including humans, animals, and fish. Timely diagnosis and treatment are crucial for the control of edwardsiellosis in the aqua industry. By using rabbit polyclonal antibody, an expression gene library of virulent Edwardsi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Palanisamy Bothammal, Mohan Ganesh, Vellaisamy Vigneshwaran, Kumarasamy Anbarasu, Karuppiah Ponmurugan, Naif Abdullah Al-Dhabi, Kalimuthusamy Natarajaseenivasan
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Edwardsiella tarda edwardsiellosis immunoreactive protein vaccine IL-10 IFN-γ Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/6179a90ecbba46d88a09b50a7f337020
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6179a90ecbba46d88a09b50a7f337020
record_format	dspace
spelling	oai:doaj.org-article:6179a90ecbba46d88a09b50a7f3370202021-11-16T05:49:14ZConstruction of Genomic Library and Screening of Edwardsiella tarda Immunogenic Proteins for Their Protective Efficacy Against Edwardsiellosis1664-322410.3389/fimmu.2021.764662https://doaj.org/article/6179a90ecbba46d88a09b50a7f3370202021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fimmu.2021.764662/fullhttps://doaj.org/toc/1664-3224Edwardsiella tarda is a severe aquaculture pathogen that can infect many hosts including humans, animals, and fish. Timely diagnosis and treatment are crucial for the control of edwardsiellosis in the aqua industry. By using rabbit polyclonal antibody, an expression gene library of virulent Edwardsiella tarda strain ED-BDU 1 isolated in south India was constructed and screened. The identified immune expressive proteins were characterized, and the corresponding coding sequences were cloned, expressed, and the purified recombinant proteins were used as antigens. The identified immunoreactive proteins namely HflC, HflK, and YhcI were studied for their immune protective potential in vivo by challenge experiments. The protective efficacy of HflC, HflK, and YhcI showed that the clearance of Edwardsiella from the host with ~ 60% survivability. Further, the immunoreactive proteins induce a strong immune response upon infection and elicit the significant production of IL-10, IFN-γ, Th1, and Th2 mediated mRNA expression and were therefore effective in vaccine production for edwardsiellosis.Palanisamy BothammalMohan GaneshVellaisamy VigneshwaranKumarasamy AnbarasuKaruppiah PonmuruganNaif Abdullah Al-DhabiKalimuthusamy NatarajaseenivasanFrontiers Media S.A.articleEdwardsiella tardaedwardsiellosisimmunoreactive proteinvaccineIL-10IFN-γImmunologic diseases. AllergyRC581-607ENFrontiers in Immunology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Edwardsiella tarda edwardsiellosis immunoreactive protein vaccine IL-10 IFN-γ Immunologic diseases. Allergy RC581-607
spellingShingle	Edwardsiella tarda edwardsiellosis immunoreactive protein vaccine IL-10 IFN-γ Immunologic diseases. Allergy RC581-607 Palanisamy Bothammal Mohan Ganesh Vellaisamy Vigneshwaran Kumarasamy Anbarasu Karuppiah Ponmurugan Naif Abdullah Al-Dhabi Kalimuthusamy Natarajaseenivasan Construction of Genomic Library and Screening of Edwardsiella tarda Immunogenic Proteins for Their Protective Efficacy Against Edwardsiellosis
description	Edwardsiella tarda is a severe aquaculture pathogen that can infect many hosts including humans, animals, and fish. Timely diagnosis and treatment are crucial for the control of edwardsiellosis in the aqua industry. By using rabbit polyclonal antibody, an expression gene library of virulent Edwardsiella tarda strain ED-BDU 1 isolated in south India was constructed and screened. The identified immune expressive proteins were characterized, and the corresponding coding sequences were cloned, expressed, and the purified recombinant proteins were used as antigens. The identified immunoreactive proteins namely HflC, HflK, and YhcI were studied for their immune protective potential in vivo by challenge experiments. The protective efficacy of HflC, HflK, and YhcI showed that the clearance of Edwardsiella from the host with ~ 60% survivability. Further, the immunoreactive proteins induce a strong immune response upon infection and elicit the significant production of IL-10, IFN-γ, Th1, and Th2 mediated mRNA expression and were therefore effective in vaccine production for edwardsiellosis.
format	article
author	Palanisamy Bothammal Mohan Ganesh Vellaisamy Vigneshwaran Kumarasamy Anbarasu Karuppiah Ponmurugan Naif Abdullah Al-Dhabi Kalimuthusamy Natarajaseenivasan
author_facet	Palanisamy Bothammal Mohan Ganesh Vellaisamy Vigneshwaran Kumarasamy Anbarasu Karuppiah Ponmurugan Naif Abdullah Al-Dhabi Kalimuthusamy Natarajaseenivasan
author_sort	Palanisamy Bothammal
title	Construction of Genomic Library and Screening of Edwardsiella tarda Immunogenic Proteins for Their Protective Efficacy Against Edwardsiellosis
title_short	Construction of Genomic Library and Screening of Edwardsiella tarda Immunogenic Proteins for Their Protective Efficacy Against Edwardsiellosis
title_full	Construction of Genomic Library and Screening of Edwardsiella tarda Immunogenic Proteins for Their Protective Efficacy Against Edwardsiellosis
title_fullStr	Construction of Genomic Library and Screening of Edwardsiella tarda Immunogenic Proteins for Their Protective Efficacy Against Edwardsiellosis
title_full_unstemmed	Construction of Genomic Library and Screening of Edwardsiella tarda Immunogenic Proteins for Their Protective Efficacy Against Edwardsiellosis
title_sort	construction of genomic library and screening of edwardsiella tarda immunogenic proteins for their protective efficacy against edwardsiellosis
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/6179a90ecbba46d88a09b50a7f337020
work_keys_str_mv	AT palanisamybothammal constructionofgenomiclibraryandscreeningofedwardsiellatardaimmunogenicproteinsfortheirprotectiveefficacyagainstedwardsiellosis AT mohanganesh constructionofgenomiclibraryandscreeningofedwardsiellatardaimmunogenicproteinsfortheirprotectiveefficacyagainstedwardsiellosis AT vellaisamyvigneshwaran constructionofgenomiclibraryandscreeningofedwardsiellatardaimmunogenicproteinsfortheirprotectiveefficacyagainstedwardsiellosis AT kumarasamyanbarasu constructionofgenomiclibraryandscreeningofedwardsiellatardaimmunogenicproteinsfortheirprotectiveefficacyagainstedwardsiellosis AT karuppiahponmurugan constructionofgenomiclibraryandscreeningofedwardsiellatardaimmunogenicproteinsfortheirprotectiveefficacyagainstedwardsiellosis AT naifabdullahaldhabi constructionofgenomiclibraryandscreeningofedwardsiellatardaimmunogenicproteinsfortheirprotectiveefficacyagainstedwardsiellosis AT kalimuthusamynatarajaseenivasan constructionofgenomiclibraryandscreeningofedwardsiellatardaimmunogenicproteinsfortheirprotectiveefficacyagainstedwardsiellosis
_version_	1718426668061687808

Construction of Genomic Library and Screening of Edwardsiella tarda Immunogenic Proteins for Their Protective Efficacy Against Edwardsiellosis

Ejemplares similares