Improving Current Knowledge on Seroprevalence and Genetic Characterization of Swine Influenza Virus in Croatian Pig Farms: A Retrospective Study

In a total of 1536 blood serum samples analysed by ELISA, antibodies for IAV nucleoprotein (NP) were detected in 30.3%. Results from HI show that the most common subtype of swIAV in the Croatian pig population was H1N1 (44.6%), followed by H3N2 (42.7%) and H1N2 (26.3%). Antibodies to at least one su...

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Autores principales: Andreja Jungić, Vladimir Savić, Josip Madić, Ljubo Barbić, Besi Roić, Dragan Brnić, Jelena Prpić, Lorena Jemeršić, Dinko Novosel
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Publicado: MDPI AG 2021
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Acceso en línea:https://doaj.org/article/617cfc28c2064dedb5b686fc750ccd2a
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spelling oai:doaj.org-article:617cfc28c2064dedb5b686fc750ccd2a2021-11-25T18:38:57ZImproving Current Knowledge on Seroprevalence and Genetic Characterization of Swine Influenza Virus in Croatian Pig Farms: A Retrospective Study10.3390/pathogens101115272076-0817https://doaj.org/article/617cfc28c2064dedb5b686fc750ccd2a2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-0817/10/11/1527https://doaj.org/toc/2076-0817In a total of 1536 blood serum samples analysed by ELISA, antibodies for IAV nucleoprotein (NP) were detected in 30.3%. Results from HI show that the most common subtype of swIAV in the Croatian pig population was H1N1 (44.6%), followed by H3N2 (42.7%) and H1N2 (26.3%). Antibodies to at least one subtype were detected in 62.19% of blood serum samples. Detection of swIAV antigen was performed by IHC and detected in 8 of 28 lung samples collected post-mortem. The matrix (M) gene was detected in nine of one hundred and forty-two lung tissue samples and in seven of twenty-nine nasopharyngeal swabs. Phylogenetic analysis of amplified HA and NA gene fragments in Croatian isolates suggests the presence of swIAV H1avN1av.Andreja JungićVladimir SavićJosip MadićLjubo BarbićBesi RoićDragan BrnićJelena PrpićLorena JemeršićDinko NovoselMDPI AGarticlepigsswine influenza virusseroprevalenceH1N1CroatiaMedicineRENPathogens, Vol 10, Iss 1527, p 1527 (2021)
institution DOAJ
collection DOAJ
language EN
topic pigs
swine influenza virus
seroprevalence
H1N1
Croatia
Medicine
R
spellingShingle pigs
swine influenza virus
seroprevalence
H1N1
Croatia
Medicine
R
Andreja Jungić
Vladimir Savić
Josip Madić
Ljubo Barbić
Besi Roić
Dragan Brnić
Jelena Prpić
Lorena Jemeršić
Dinko Novosel
Improving Current Knowledge on Seroprevalence and Genetic Characterization of Swine Influenza Virus in Croatian Pig Farms: A Retrospective Study
description In a total of 1536 blood serum samples analysed by ELISA, antibodies for IAV nucleoprotein (NP) were detected in 30.3%. Results from HI show that the most common subtype of swIAV in the Croatian pig population was H1N1 (44.6%), followed by H3N2 (42.7%) and H1N2 (26.3%). Antibodies to at least one subtype were detected in 62.19% of blood serum samples. Detection of swIAV antigen was performed by IHC and detected in 8 of 28 lung samples collected post-mortem. The matrix (M) gene was detected in nine of one hundred and forty-two lung tissue samples and in seven of twenty-nine nasopharyngeal swabs. Phylogenetic analysis of amplified HA and NA gene fragments in Croatian isolates suggests the presence of swIAV H1avN1av.
format article
author Andreja Jungić
Vladimir Savić
Josip Madić
Ljubo Barbić
Besi Roić
Dragan Brnić
Jelena Prpić
Lorena Jemeršić
Dinko Novosel
author_facet Andreja Jungić
Vladimir Savić
Josip Madić
Ljubo Barbić
Besi Roić
Dragan Brnić
Jelena Prpić
Lorena Jemeršić
Dinko Novosel
author_sort Andreja Jungić
title Improving Current Knowledge on Seroprevalence and Genetic Characterization of Swine Influenza Virus in Croatian Pig Farms: A Retrospective Study
title_short Improving Current Knowledge on Seroprevalence and Genetic Characterization of Swine Influenza Virus in Croatian Pig Farms: A Retrospective Study
title_full Improving Current Knowledge on Seroprevalence and Genetic Characterization of Swine Influenza Virus in Croatian Pig Farms: A Retrospective Study
title_fullStr Improving Current Knowledge on Seroprevalence and Genetic Characterization of Swine Influenza Virus in Croatian Pig Farms: A Retrospective Study
title_full_unstemmed Improving Current Knowledge on Seroprevalence and Genetic Characterization of Swine Influenza Virus in Croatian Pig Farms: A Retrospective Study
title_sort improving current knowledge on seroprevalence and genetic characterization of swine influenza virus in croatian pig farms: a retrospective study
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/617cfc28c2064dedb5b686fc750ccd2a
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